Midi gels

Cells were lysed using RIPA buffer (Cell Signaling, Danvers, MA, USA) and ultra sound exposure. Proteins were separated on Midi gels (Bio-Rad, Munich, Germany), blotted onto a PVDF membrane (Bio-Rad) using Trans-Blot® Turbo™ Transfer System (Bio-Rad, 2.5 A, 25 V, 10 min), blocked in LI-COR (Lincoln, NE, USA) blocking buffer and detected via LI-COR Odyssey Imaging System and Image Studio Lite software. Antibodies are listed in Additional file 1: Table S1. For quantification, band intensities were assessed using Image Studio Lite software. Values were normalized to respective GAPDH control bands run on each individual gel and ratios between phosphorylated and total protein are given below the images. Mean values and standard deviations of three individual experiments were calculated and displayed as bar charts.

Cells were lysed using RIPA buffer (Cell Signaling) and ultra sound exposure. Proteins (30 μg) were separated on Midi gels (Bio-Rad, Munich, Germany), blotted onto a PVDF membrane (Bio-Rad) using the Trans-Blot® Turbo™ Transfer System (Bio-Rad, 2.5 A, 25 V, 10 min), blocked in LI-COR (Lincoln, NE, USA) blocking buffer and detected via LI-COR Odyssey Imaging System and Image Studio Lite software. The following antibodies were used for protein detection: pAKT (Ser473), AKT, BCL6 (clone D4I2V), BACH2 (clone D3T3G; all Cell Signaling), GAPDH (clone ZG003; Invitrogen), IRDye® 680RD Goat anti-Mouse, IRDye® 800CW Goat anti-Mouse, IRDye® 680RD Goat anti-Rabbit and IRDye® 800CW Goat anti-Rabbit (all LI-COR). Experiments were carried out in biological triplicates.