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Tunel assay reagent

Manufactured by Merck Group
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Sourced in Germany

The TUNEL assay reagent is a laboratory tool used to detect and quantify apoptosis, or programmed cell death, in cell samples. The reagent facilitates the labeling of DNA fragmentation, a hallmark of apoptosis, allowing for the identification and analysis of cells undergoing this process. The core function of the TUNEL assay reagent is to provide a reliable method for the detection and measurement of apoptosis in various biological applications.

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Lab products found in correlation

Optical microscopy, by Olympus (2 mentions)

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TUNEL) assay (47 citations) TUNEL reagent (20 citations)

3 protocols using tunel assay reagent

1

Apoptosis Analysis in Hippocampal Neurons

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TUNEL assay reagent was obtained from Merck KGaA (Darmstadt, Germany). According to the kit instructions, TUNEL positive cells in hippocampal neurons were analyzed under microscopy.
Zhan Y., Han J., Xia J, & Wang X. (2021). Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis. Neuropsychiatric Disease and Treatment, 17, 613-626.
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2

TUNEL Assay for Apoptosis in SCC-4 and SCC-9

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The transfected cell samples of SCC-4 and SCC-9 were washed employing PBS and fixed using 4% PFS for TUNEL assay [19 (link)], in the presence of TUNEL assay reagent (Merck KGaA, Darmstadt, Germany). Following addition of DAPI staining solution, cell samples were analyzed using optical microscopy (Olympus). Each samples were assayed for more than triplicate.
Fu S.W., Zhang Y., Li S., Shi Z.Y., Zhao J, & He Q.L. (2020). LncRNA TTN-AS1 promotes the progression of oral squamous cell carcinoma via miR-411-3p/NFAT5 axis. Cancer Cell International, 20, 415.
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3

TUNEL Assay for Apoptosis in LUAD

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The transfected LUAD cell samples were rinsed utilizing PBS for fixing. Then, the TUNEL assay reagent (Merck KGaA, Darmstadt, Germany) was used for staining the apoptotic cells. After DAPI staining of cell nuclei, the optical microscopy (Olympus) was applied for analysis.
Meng F., Zhou Y., Dong B., Dong A, & Zhang J. (2020). Long non-coding RNA LINC01194 promotes the proliferation, migration and invasion of lung adenocarcinoma cells by targeting miR-641/SETD7 axis. Cancer Cell International, 20, 588.
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