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Td 08485

Manufactured by Inotiv
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Sourced in Montenegro, Spain, United Kingdom, United States

The TD.08485 is a laboratory instrument designed for the analysis of various samples. It operates by utilizing a specific measurement technique to provide data and information about the properties or composition of the samples under investigation. The core function of this product is to perform analytical tasks within a controlled laboratory environment, but a detailed description of its intended use or interpretation of its capabilities is not available.

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Lab products found in correlation

Td 88137, by Inotiv (11 mentions) C57bl 6j mice, by Jackson ImmunoResearch (4 mentions) Td02028, by Inotiv (2 mentions) Global no 2918, by Inotiv (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Live mice analyzer lf50, by Bruker (1 mentions) Trp53tm1tyj, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions) Control diet, by Inotiv (1 mentions)

16 protocols using td 08485

1

Atherosclerosis Model in ApoE-/- Mice

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Eight-week-old male ApoE-/- mice (C57BL/6) from Cavens Lab Animal (Changzhou, China) were used to generate an atherosclerosis model. Those mice were randomized into 3 groups: normal chow diet (NCD, TD08485, Harlan Teklad) (n=10), high-fat diet (HFD, TD02028, Harlan Teklad) (n=10), and nicotine + HFD (n=10). To monitor and analyze the influence of nicotine, mice in the nicotine+ HFD group were treated with 2 mg·kg-1·day-1 of nicotine via subcutaneous injection, while those in the NCD and HFD groups were injected with an equal volume of phosphate buffer saline (PBS). The treatment lasted for 12 weeks. All mice were ultimately euthanized for further analysis. All animal experiments were previously approved by the Animal Care and Use Committee at the Research Institute of Medicine of Shanghai Jiao Tong University.
Zhu J., Liu B., Wang Z., Wang D., Ni H., Zhang L, & Wang Y. (2019). Exosomes from nicotine-stimulated macrophages accelerate atherosclerosis through miR-21-3p/PTEN-mediated VSMC migration and proliferation. Theranostics, 9(23), 6901-6919.
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2

Metabolic Effects of Dapagliflozin in Obese Mice

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Ten-week-old male C57BL/6J mice were obtained from the Jackson Laboratories and maintained on a 12 h light/12 h dark cycle. They were fed Western diet (WD: 42% milkfat, 34% sucrose, and 0.2% cholesterol, Harlan Teklad, TD.88137) or a matched low-fat diet (LFD: 10 kcal % fat, Harlan Teklad TD.08485) that had or did not have dapagliflozin (5 mg/kg per body weight/day) added to them. Following the 26-week treatment period the animals were sacrificed.
Food intake and body weight were measured every 2 weeks. Mice were placed in metabolic cages for 24-h urine collection for measurement of volume, creatinine, and albumin content. Blood pressure was measured using the tail artery technique with the BP-2000 Blood Pressure Analysis SystemTM (Visitech Systems, Inc., Apex, NC, USA). At the end of the study period, the mice were anesthetized, and blood was drawn for measurement of glucose, triglyceride, and cholesterol.
Animal studies and related protocols were approved by the Animal Care and Use Committee (B-63914(11)2E, 3 September 2014) at the University of Colorado Denver. All animal experimentation was conducted in accordance with the Guide for Care and Use of Laboratory Animals (National Institutes of Health, Bethesda, MD, USA).
Wang D., Luo Y., Wang X., Orlicky D.J., Myakala K., Yang P, & Levi M. (2018). The Sodium-Glucose Cotransporter 2 Inhibitor Dapagliflozin Prevents Renal and Liver Disease in Western Diet Induced Obesity Mice. International Journal of Molecular Sciences, 19(1), 137.
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3

EAS Model Construction in ApoE-/- Mice

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Eight-week-old male ApoE−/− mice (C57BL/6) were obtained from Cavens Lab Animal (Changzhou, China) and used to construct the EAS model. Those mice were randomized into a control group fed a normal chow diet (NCD, TD08485, Harlan Teklad) (n = 5) and an EAS group fed a high-fat diet (HFD, TD02028, Harlan Teklad) (n = 5). All mice were sacrificed after 4 weeks of feeding for further analysis. Due to the difficulty of operation, the number of models successfully constructed and the samples obtained are as follows: NCD = 3, HFD = 4. The successful construction of the EAS animal model was verified by oil red O staining (E607319-0010, Sangon Biotech) on part of the arterial tissues. All animal experiments were approved by the Institutional Animal Care and Use Committee of Shanghai General Hospital.
Xing C., Jiang Z, & Wang Y. (2022). Downregulation of NAGLU in VEC Increases Abnormal Accumulation of Lysosomes and Represents a Predictive Biomarker in Early Atherosclerosis. Frontiers in Cell and Developmental Biology, 9, 797047.
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4

Atherosclerosis Progression in Apoe-/-;D5-/- Mice

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The Apoe-null (Apoe−/−) mice in the C57BL/6 background were purchased from the Jackson Laboratory (Bar Harbor, ME; Cat. No. 002052). The D5−/− mice has been extensively studies and kindly provided by O’Brien et al [Galli et al., 2008 (link); Onal et al., 2012 (link); Onal et al., 2016b (link)]. The Rankl−/− [Kim et al., 2000 (link)] and Rankl−/−;Tg transgenic mice [Onal et al., 2014a ] in the C57BL/6 background have been described previously. The Apoe−/− mice were crossed with the D5−/− mice to generate the Apoe−/−;D5−/− mice. Only female mice were used for this study. Upon weaning mice were placed on a low fat control diet (Harlan Teklad, Madison, WI; TD.08485) up to start of the study. In the beginning of the study, all mice at 7 weeks of age were placed on a high-fat diet (HFD) (Harlan Teklad, Madison, WI; TD.88137) for a further 12 or 18 weeks to promote atherosclerosis. All animal studies were reviewed and approved by the University of Wisconsin-Madison Animal Care and Use Committee.
Shamsuzzaman S., Onal M., St John H.C, & Pike J.W. (2017). Deletion of a Distal RANKL Gene Enhancer Delays Progression of Atherosclerotic Plaque Calcification in Hypercholesterolemic Mice. Journal of cellular biochemistry, 118(12), 4240-4253.
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5

Aging and Diet Effects on Mouse T Cells

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Male and female C57BL/6 mice were obtained from The Jackson Laboratory (Stock 664) or the National Institute on Aging. T cell receptor delta chain knockout mice (TCRδ KO, B6.129P2-Tcrdtm1Mom/J, Stock 2120) were obtained from The Jackson Lab and bred in-house. Mice were euthanized as young-adults (4–7 months old) or at old age (19–24 months old); age is specified for each experiment in figure legends. Mice were housed in pressurized intraventilated cages and maintained in an environment under controlled temperature (21–23 °C), humidity (30–70%), and lighting (14 h/10 h, light/dark) with free access to drinking water and chow (Teklad Global No. 2918). A subset of mice was subjected to high-fat feeding (Teklad TD.88137 42% kcal from fat) alongside control diet-fed mice (Teklad TD.08485, 13% kcal from fat); diets were given ad libitum. All procedures were approved by the Institutional Animal Care and Use Committee at the University of Kentucky, and performed in accord with the National Institutes of Health guidelines for ethical animal treatment.
Bruno M.E., Mukherjee S., Powell W.L., Mori S.F., Wallace F.K., Balasuriya B.K., Su L.C., Stromberg A.J., Cohen D.A, & Starr M.E. (2022). Accumulation of γδ T cells in visceral fat with aging promotes chronic inflammation. GeroScience, 44(3), 1761-1778.
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6

Mice Liver Imaging on High Fat Diet

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Male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME) fed a 42% milkfat, 34% sucrose, and fed with 0.2% cholesterol, Harlan Teklad, TD.88137 WD, or a matched 10% fat, Harlan Teklad TD.08485 LF. We imaged flash frozen liver slices (5 μm thick), embedded in OCT, obtained from mice that were fed with a low-fat diet (LF) or high fat high cholesterol Western diet (WD)35 .
Vallmitjana A., Torrado B., Dvornikov A., Ranjit S, & Gratton E. (2020). Blind Resolution of Lifetime Components in Individual Pixels of Fluorescence Lifetime Images Using the Phasor Approach. The journal of physical chemistry. B, 124(45), 10126-10137.
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7

Long-Term Dietary Intervention Study

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Western (Teklad, TD88137) and Low Fat Control (Teklad, TD08485) diets were administered ad libitum to 9 week old animals for a total duration of the challenge of 32 weeks. Fat and lean mass was calculated by time-domain nuclear magnetic resonance (TD-NMR) by using a minispec Live Mice Analyzer LF50 (Bruker) the day of the culling. Serum and tissue collection were performed in fed condition the day of the culling.
Vacca M., Leslie J., Virtue S., Lam B.Y.H., Govaere O., Tiniakos D., Snow S., Davies S., Petkevicius K., Tong Z., Peirce V., Nielsen M.J., Ament Z., Li W., Kostrzewski T., Leeming D.J., Ratziu V., Allison M.E.D., Anstee Q.M., Griffin J.L., Oakley F, & Vidal-Puig A. (2020). Bone morphogenetic protein 8B promotes the progression of non-alcoholic steatohepatitis. Nature metabolism, 2(6).
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8

High-fat diet effects in Trp53 knockout mice

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Male C57BL/6 and male C57BL/6 Trp53 null mice (Trp53tm1Tyj) were purchased from Jackson Laboratory. One-month old C57BL/6 and Trp53tm1Tyj were fed either a standard diet (TD.08485, Envigo, Barcelona, Spain) or a high-fat diet with 42% calories from fat (TD.88137, Envigo, Barcelona, Spain) for 12 weeks. Body weight and food intake were monitored during these 12 weeks. On the other hand, male C57BL/6 Srxn1 null mice were generated as previously described [31 ] and were fed standard diet.
All animals were housed under standard environmental conditions (23 ± 1°C, 60% relative humidity, and constant 12 h/12h light/darkness cycles), with food and water ad libitum, at the animal housing facilities from the SCSIE located at the Faculty of Pharmacy from the Universitat de València. All procedures and experiments were conducted in compliance with the legislation on protection of animals used for scientific purposes in Spain (RD 53/2013) and the EU (Directive 2010/63/EU). The study was approved by the Ethics Committee of Animal Experimentation and Welfare of the University of Valencia (Valencia, Spain) (Protocol codes: 2020/VSC/PEA/0030; 2020/VSC/PEA/0030; and 2021/VSC/PEA/0216).
Rius-Pérez S., Pérez S., Toledano M.B, & Sastre J. (2022). p53 drives necroptosis via downregulation of sulfiredoxin and peroxiredoxin 3. Redox Biology, 56, 102423.
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9

Diet-Induced Obesity and Dry Eye

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Specific-pathogen-free female C57BL/6J mice without eye disease, aged 6 weeks and 12 weeks, were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA). Six-week-old mice were fed either normal chow rodent diet (ND) (Teklad global 2019 rodent diet), sterile isocaloric low-fat diet (LFD) (TD08485, Envigo), or sterile 21% milk Western Style high-fat diet (HFD) (TD88137, Envigo) from 6 weeks of age for 6 weeks. Mice from each group were measured for weight, fasting blood glucose levels, and tear production using phenol red thread test at the end of the feeding period at 12 weeks. All animal protocols were approved by the Animal Care Committee of the University of Illinois at Chicago (UIC). All animals were treated in accordance with the Association for Research in Vision and Ophthalmology’s Statement for the Use of Animals in Ophthalmology and Vision Research and the guidelines of the Animal Care Committee at the University of Illinois at Chicago.
Kang K., Zhou Q., McGinn L., Nguyen T., Luo Y., Djalilian A, & Rosenblatt M. (2021). High fat diet induced gut dysbiosis alters corneal epithelial injury response in mice. The ocular surface, 23, 49-59.
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10

High-Fat Diet Induced Obesity in Mice

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Two-month old male mice (C57BL/6J) were fed ad libitum either a normal diet (ND, n = 10) containing 12.6% kCal from fat (Envigo TD.08485) or a high fat diet (HFD, n = 15) containing 42% kcal from fat (Envigo TD.88137) for a period of 16 weeks. The mice were weighed once a week. At the end of the 16 weeks, all mice were euthanized. The Animal Studies Committee at Duke University approved all mouse procedures. All additional methods were carried out in accordance with relevant guidelines and regulations.
Collins A.T., Hu G., Newman H., Reinsvold M.H., Goldsmith M.R., Twomey-Kozak J.N., Leddy H.A., Sharma D., Shen L., DeFrate L.E, & Karner C.M. (2021). Obesity alters the collagen organization and mechanical properties of murine cartilage. Scientific Reports, 11, 1626.
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