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Yeast extract

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Yeast extract is a nutritional supplement derived from the cellular contents of yeast. It provides a source of amino acids, vitamins, and minerals essential for microbial growth and metabolism. The extract is commonly used in growth media for culturing various microorganisms in laboratory settings.

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Lab products found in correlation

Peptone, by Condalab (7 mentions) Tryptone, by Condalab (5 mentions) Bacteriological agar, by Condalab (3 mentions) Menadione, by Merck Group (2 mentions) Hemin, by Merck Group (2 mentions) Citric acid, by Thermo Fisher Scientific (2 mentions) D glucose, by Thermo Fisher Scientific (2 mentions) Sodium di basic hydrogen phosphate, by Merck Group (2 mentions) Anaeropack rectangular jar, by Mitsubishi (1 mentions) Sodium hydroxide (naoh), by ITW Reagents (1 mentions) Mgso4 7h2o, by Avantor (1 mentions) Sodium chloride (nacl), by ITW Reagents (1 mentions) Nahco3, by ITW Reagents (1 mentions) Nh4cl, by ITW Reagents (1 mentions) Nah2po4, by ITW Reagents (1 mentions) Cdcl2 2.5h2o, by Merck Group (1 mentions) Na2hpo4, by ITW Reagents (1 mentions) Potassium chloride (kcl), by ITW Reagents (1 mentions) Mgcl2 6h2o, by Avantor (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions)

20 protocols using yeast extract

1

Cultivation of Yeast Strains on YPD Agar

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The solid culture medium
used was YPD-agar. It contains 1% yeast extract (Condalab, Madrid,
Spain), 2% peptone (Condalab, Madrid, Spain), 2% pure anhydrous glucose
(PanReac, Barcelona, Spain), and 1.7% agar (Condalab, Madrid, Spain).
Incubation of the yeast seeded Petri dishes was carried out at 26
°C in an oven (J.P Selecta, Barcelona, Spain). Colony forming
units (CFU/mL) were counted by preparing serial dilutions in sterile
distilled water and plating 10–5 and 10–7 dilutions on YPD-agar plates. In all cases the cell count was around
8-log CFU/mL.
For biomass growth of the different yeasts, a
YPD liquid culture was prepared. It also contains 1% yeast extract
(Condalab, Madrid, Spain), 2% peptone (Condalab, Madrid, Spain), and
2% pure anhydrous glucose (PanReac, Barcelona, Spain). Two passages
were performed prior to inoculation of the beer wort, the first in
glass tubes with a volume of 5–10 mL of medium and the second
in Erlenmeyer flask with 40% YPD medium. The amount of yeast inoculated
at the different stages of the process corresponded to 2% of the final
volume. The glass tubes with YPD medium were incubated at 26 °C
for 24 h in a static incubator (J.P. Selecta, Barcelona, Spain), while
the cultures in Erlenmeyer flasks, were incubated at 26 °C in
an incubator with orbital shaking at 115 rpm (New Brunswick Innova
40/40R, Eppendorf, Barcelona, Spain) for 48 h.
Peces-Pérez R., Vaquero C., Callejo M.J, & Morata A. (2022). Biomodulation of Physicochemical Parameters, Aromas, and Sensory Profile of Craft Beers by Using Non-Saccharomyces Yeasts. ACS Omega, 7(21), 17822-17840.
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2

Oral Infection with Porphyromonas gingivalis Strains

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Following one-week acclimatization, the experiments started with 7 weeks old animals and were developed according to Baker et al. with slight modifications [3 (link)]. Animals were randomly divided into three groups according to their treatment: Control, P. gingivalis W83 and P. gingivalis TDC60 (n = 6 mice/group). Animals were cohoused according to their group to avoid horizontal transmission (3 mice/cage). Prior to each infection, the bacteria were grown in enriched brain-heart infusion (BHI) broth containing, per liter, 37 g of BHI powder (AES Chemunex, France), 5 g of yeast extract (Conda, Dutscher), 25 mg of hemin (Sigma), and 10 mg of menadione (Sigma), placed in an AnaeroPack™ rectangular jar (Mitsubishi Gas Chemical Co. Inc., Tokyo, Japan) with an AnaeroGen™ 3.5 L (Mitsubishi Gas Chemical Co. Inc.) for 24 h at 37°C to reach the mid-exponential phase. The W83- and TDC60-infected groups received 109 CFU of live P. gingivalis of the corresponding strain, resuspended in 100 µL of PBS. The infection was made by direct inoculation of the oral cavity, three times a week, during 5 weeks. Controls were sham-infected mice which received the PBS, but no P. gingivalis. The oral administrations were made at the same time for all groups.
Boyer E., Leroyer P., Malherbe L., Fong S.B., Loréal O., Bonnaure Mallet M, & Meuric V. (2020). Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice. Journal of Oral Microbiology, 12(1), 1832837.
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3

Culturing Porphyromonas gingivalis ATCC 33277

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Porphyromonas gingivalis ATCC 33277 was cultured in enriched BHI broth containing, per liter, 37 g of BHI powder (AES Chemunex, France), 5 g of yeast extract (Conda, Dutscher) 25 mg of hemin (Sigma), and 10 mg of menadione (Sigma). The strains were maintained on Columbia 3 agar plates supplemented with 5% (v/v) defibrinated horse blood (AES Chemunex, Combourg, France), 25 mg/l of hemin, and 10 mg/l of menadione. The cultures were incubated at 37°C in an anaerobic chamber Macs-VA500 (Don Whitley) flooded with 80% N2, 10% H2 and 10% CO2. However, brief exposures to oxygen outside the chamber cannot be avoided for some experiments. The media were supplemented with erythromycin 5 μg/ml, and tetracycline 1 μg/ml when required. The P. gingivalis cydAB deletion mutant was constructed and is described by Leclerc et al. [19 (link)]. This mutant is resistant to erythromycin. The exact role of the cydW gene that is part of the cydWAB operon is unknown. Therefore we have not deleted the gene to study the phenotype of a cytochrome bd oxidase mutant.
Leclerc J., Rosenfeld E., Trainini M., Martin B., Meuric V., Bonnaure-Mallet M, & Baysse C. (2015). The Cytochrome bd Oxidase of Porphyromonas gingivalis Contributes to Oxidative Stress Resistance and Dioxygen Tolerance. PLoS ONE, 10(12), e0143808.
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4

Hfx. mediterranei Cd(II) Biosorption Protocol

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For all the dilutions prepared, high-purity water (resistivity > 18 MΩ) from a Direct-Q 3V water purification system (Millipore Inc., Paris, France) was used.
For the incubation of Hfx. mediterranei, saltwater was prepared using CaCl2·H2O (Labken, Barcelona, Spain), KCl, NaBr, NaCl and NaHCO3 (Panreac, Castellar del Valles, Spain), and MgCl2·6H2O and MgSO4·7H2O (VWR Chemicals, Radnor, PA, USA). Two different incubation media were prepared from the saltwater: CM by adding yeast extract (Condalab, Madrid, Spain) and DM by adding NH4Cl, D(±)-Anhydrous glucose, Na2HPO4, NaH2PO4 (Panreac, Castellar del Valles, Spain), and Tris(hydroxymethyl)-aminomethane and FeCl3 from (Merck, Darmstadt, Germany). Cd (II) stock solution was prepared using the salt CdCl2·2.5 H2O (Merck, Darmstadt, Germany), and the pH of the medium was adjusted using HCl (Merck, Darmstadt, Germany) and NaOH (Panreac, Castellar del Valles, Spain).
Finally, for ICP analysis, sample digestion was conducted using 69% HNO3 (Panreac, Castellar del Valles, Spain) and the calibration standard solutions were prepared from the ICP-IV multi-elemental standard as well as Mo, Sc and Ru monoelemental standards, all reagents and purchased from Merck (Darmstadt, Germany).
Saez-Zamacona I., Grindlay G, & Martínez-Espinosa R.M. (2023). Evaluation of Haloferax mediterranei Strain R4 Capabilities for Cadmium Removal from Brines. Marine Drugs, 21(2), 72.
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5

Glucose and Citric Acid Protocol

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D-Glucose and citric acid were purchased from Fisher chemical; peptone, yeast extract and agar were Conda Lab supplies; and the sodium di-basic hydrogen phosphate was provided from Sigma Aldrich and used as received.
Al-Hagar O.E, & Abol-Fotouh D. (2022). A turning point in the bacterial nanocellulose production employing low doses of gamma radiation. Scientific Reports, 12, 7012.
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6

Synthetic Brine Preparation for Mold Volatile Organic Compounds

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For the preparation of the synthetic brine, NaCl (Sharlau, Spain), lactic acid (Sharlau, Spain), glucose (Sharlau, Spain), and yeast extract (Condalab, Spain) were used. YPD agar (Condalab, Spain) was used for the growth of the altering molds. Commercial standard volatile organic compounds (VOC) solutions were used for the volatile organic compounds studied [propanoic acid, butanoic acid, 3,5-dimethyl-benzenemethanol, 2-methoxy-phenol, octanal, dodecanal, 2-methyl-butanoic acid, butyl ester butanoic acid, pentadecane, 3-methyl-1-butanol, 1-ethylpropyl-benzene, hexanal, heptanal, 2-nonanone, benzaldehyde, nonanal, 2,4-dimethyl-benzaldehyde, (E)-2-decenal, and a-murolene]. All these chemical reagents were purchased from Fisher Scientific (Fisher Scientific, MO, United States).
Sánchez R., Pérez-Nevado F., Montero-Fernández I., Lozano J., Meléndez F, & Martín-Vertedor D. (2022). Application of Electronic Nose to Discriminate Species of Mold Strains in Synthetic Brines. Frontiers in Microbiology, 13, 897178.
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7

Yeast Genetic Deletion Library Characterization

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The S. cerevisiae strains herein used were wild-type strain BY4741 (MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) and 17 haploid deletion mutants strains in the BY4741 background generated by the Saccharomyces Genome Deletion Project, and obtained from the European S. cerevisiae Archive for Functional Analysis (EUROSCARF). The following haploid deletion mutants strains of BY4741 were used in this work: Y04034 (∆bmh2), Y00302 (∆prb1), Y00226 (∆ilv1), Y06396 (∆met6), Y04394 (∆trp5), Y04569 (∆aro8), Y04822 (∆tdh3),Y05887 (∆yhb1), Y06933 (∆hom6), Y05217 (∆imh1), Y07706 (∆ssb2), Y05376 (∆cit1), Y01749 (∆dpp3), Y01620 (∆pro2), Y01672 (∆gdh1), Y02114 (∆spp1) and Y05493 (∆tkl1).
Strains were grown in YPD medium [2% (w/v) glucose (Scharlab, Barcelona, Spain), 1% (w/v) yeast extract (Conda, Madrid, Spain) and 2% (w/v) peptone (Conda)] with 2% (w/v) agar (Scharlab) (for plates only). Strains were streaked from a frozen glycerol stock to a fresh liquid YPD medium and grown at 28 °C overnight with shaking. These cultures were diluted in fresh liquid YPD medium and grown again at 28 °C overnight with shaking. They were then spread over YPD agar plates and incubated at 28 °C for 72 h.
Peláez-Soto A., Roig P., Martínez-Culebras P.V., Fernández-Espinar M.T, & Gil J.V. (2020). Proteomic Analysis of Saccharomyces cerevisiae Response to Oxidative Stress Mediated by Cocoa Polyphenols Extract. Molecules, 25(3), 452.
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8

Yeast Strain Characterization in Varied Mediums

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The S. cerevisiae strains W303–1A (ura3–1; trp1–1; leu2–3112; his3–11; ade2–1; can1–100) (ATCC 208352) and CEN.PK2–1D (ura3–52; trp1–289; leu2–3112; his3Δ 1; MAL2-8C; SUC2) were used as indicated in the text and figures. Cells were grown in YPAU media (1% yeast extract (Conda), 2% bactopeptone (Pronadisa), 0.2 mg/ml adenine sulfate (Formedium) and 0.27 mg/ml uracil (Amresco)) with 2% glucose (Sigma), 2% galactose (Formedium) or 3% glycerol (GPR Rectapur VWR) as described in each case.
Pérez-González A., Kniewel R., Veldhuizen M., Verma H.K., Navarro-Rodríguez M., Rubio L.M, & Caro E. (2017). Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria. BMC Biotechnology, 17, 80.
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9

Isolation of Potent BNC Producers

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Eleven samples of garden flowers, rotten fruits, fermented foods, fermented beverage, and vinegar remnants were collected to isolate BNC producing strains. The production was carried out on Hestrin-Schramm33 (link) (HS) medium consists of (g/L): D-Glucose (20), peptone (5), yeast extract (5), sodium di-basic hydrogen phosphate (2.7), and citric acid (1.15). D-Glucose and citric acid were purchased from Fisher chemical; peptone, yeast extract and agar were purchased from Conda Lab; and the sodium di-basic hydrogen phosphate was provided from Sigma Aldrich and used as received. The 11 Erlenmeyer flasks were incubated at 28 °C for 10 days. The positive cultures that showed floating gel-like pellicles underwent purification procedures to isolate pure BNC-producing bacterial isolates for selecting the potent BNC producer.
Abol-Fotouh D., Hassan M.A., Shokry H., Roig A., Azab M.S, & Kashyout A.E. (2020). Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1. Scientific Reports, 10, 3491.
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10

Anaerobic Growth of Faecalibacterium prausnitzii

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F. prausnitzii (strain A2-165) was grown overnight at 37°C in an anaerobic (90 % N2, 5% CO2 and 5% H2) workstation (Whitley A35 anaerobic workstation) in LyBHI broth, 37 g. L−1 of brain-heart infusion (Difco) and 5 g. L−1 of yeast extract (Conda) at pH 7. The culture supernatant of F. prausnitzii was obtained by centrifugation at 1700 g at 4°C for 20 min.
Quévrain E., Maubert M.A., Michon C., Chain F., Marquant R., Tailhades J., Miquel S., Carlier L., Bermúdez-Humarán L.G., Pigneur B., Lequin O., Kharrat P., Thomas G., Rainteau D., Aubry C., Breyner N., Afonso C., Lavielle S., Grill J.P., Chassaing G., Chatel J.M., Trugnan G., Xavier R., Langella P., Sokol H, & Seksik P. (2015). Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii, a commensal bacterium deficient in Crohn's disease. Gut, 65(3), 415-425.
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