Western blotting analysis was conducted as previously described3 (link). Antibodies of HPSE (ab232817), GAPDH (ab181602), MLKL (ab184718), phosphorylated MLKL (p-MLKL) (ab196436), TNFR1(ab223352), TRADD (ab238960), caspase-8 (ab227430) and FADD (ab108601) were purchased from Abcam (Cambridge, MA, USA). SDC-1 (10593-1-AP), TNF-α (60291-1-AP) antibodies were from Proteintech Group, Inc (Rosemont, IL, USA). RIPK1 (A7414), RIPK3 (A12996), NF-κB (A19653), p-NF-κB(AP0124), p38 MAPK (A14401), and p-p38 MAPK (AP0526) antibodies were from ABclonal Technology (Woburn, MA, USA).
Ab108601
Manufactured by Abcam
Sourced in United States
Ab108601 is a recombinant monoclonal antibody that specifically recognizes the human EGFR protein. It is suitable for use in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab184718, by Abcam (2 mentions)
Ab223352, by Abcam (1 mentions)
Ab227430, by Abcam (1 mentions)
Ab181602, by Abcam (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Ab209384, by Abcam (1 mentions)
Ab187091, by Abcam (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Cleaved gsdmd, by Cell Signaling Technology (1 mentions)
Gsdmd, by Cell Signaling Technology (1 mentions)
2 protocols using ab108601
1
Comprehensive Western Blotting Analysis
2
Immunoblotting Analysis of Necroptosis Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissues and THP-1 cells were lysed in RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche). Proteins were then separated by 8–10% SDS-PAGE and transferred to a PVDF membrane. The membrane was first blocked in 5% milk, and then incubated overnight at 4°C with primary antibodies against p-MLKL (1:1000, ab187091, Abcam), MLKL (1:1000, ab184718, Abcam), p-RIPK3 (1:1000, ab209384, Abcam), RIPK3 (1:1000, #2238, Prosci), FADD (1:1000, ab108601, Abcam), Cleaved Caspase-3 (1:1000, #9664, Cell Signaling Technology), Cleaved GSDM-D (1:1000, #36425, Cell Signaling Technology), GSDM-D (1:1000; #39754, Cell Signaling Technology), p-S6 (1:1000, #5364, Cell Signaling Technology) and ACTIN (1:5000; #3700, Cell Signaling Technology). Subsequently, the membrane was incubated with a secondary antibody for another 1 h. Images were visualized by Tanon image system (Tanon Technology Co., Shanghai, China).
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