Isolera one purification system

Manufactured by Biotage

The Isolera One purification system is a compact, automated flash chromatography system designed for efficient and reliable separation and purification of chemical compounds. It utilizes a high-performance solvent delivery system and integrated UV/VIS detector to facilitate the isolation and recovery of target analytes from complex mixtures.

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Lab products found in correlation

Pu 2080, by Jasco (3 mentions) Acquity uplc h class system, by Waters Corporation (3 mentions) Xevo tqd, by Waters Corporation (2 mentions) Ods 3, by GL Sciences (2 mentions) Acquity uplc beh c18 1.7 μm, by Waters Corporation (2 mentions) Snap ultra c18 column, by Biotage (1 mentions) Jnm ecz400 spectrometer, by JEOL (1 mentions) 1290 uplc lcms, by Agilent Technologies (1 mentions) Acquity uplc beh c18 1.7 μm 2 1 50 mm column, by Waters Corporation (1 mentions) Jnm la400, by JEOL (1 mentions) Jnm la400 spectrometer, by JEOL (1 mentions)

4 protocols using isolera one purification system

Comprehensive Analytical Techniques for Chemical Compounds

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NMR spectra were recorded using a JEOL JNM-ECZ400 spectrometer at 400 MHz for ¹H NMR and 100 MHz for ¹³C NMR. Mass spectra (MS) were obtained using JEOL JMS-T100LP AccuTOF LC-plus 4 G (ESI).
Preparative HPLC was performed on an Inertsil ODS-3 (10.0 × 250 mm) column (GL Sciences Inc.) using an HPLC system composed of a pump (PU-2080, JASCO) and a detector (MD-2015). Preparative MPLC was performed on an Isolera One purification system (Biotage) equipped with a Biotage SNAP Ultra C18 column (for reverse phase separation) or on an MPLC system comprising a pump and detector (EPCLC AI-580S, Yamazen) and equipped with a silica gel column (silica gel 40 μm or Amino 40 μm, Yamazen) (for normal phase separation). LC-MS analysis was performed on an Acquity UPLC H-Class system (Waters) equipped with an Acquity UPLC BEH C18 1.7 μm (2.1 × 50 mm) column (Waters) and an MS detector (QDa or Xevo TQD, Waters).

Yanagi K., Komatsu T., Fujikawa Y., Kojima H., Okabe T., Nagano T., Ueno T., Hanaoka K, & Urano Y. (2023). Development of pathway-oriented screening to identify compounds to control 2-methylglyoxal metabolism in tumor cells. Communications Chemistry, 6, 68.

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Chiral Compound Purification and Characterization

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Chemicals and reagents were purchased from commercial sources and used without further purification. The proton NMR spectrum was recorded on a Bruker 400 MHz (100 MHz) spectrometer equipped with a cryogenic probe. Proton chemical shifts are reported in ppm (δ) relative to tetramethylsilane and calibrated using the residual solvent resonance (DMSO, δ 2.50 ppm). The NMR spectrum was recorded at ambient temperature (about 25°C). Preparative reversed-phase chromatography was performed on a Biotage Isolera One purification system, using C-18 silica as the stationary phase, with CH₃OH as the strong solvent and H₂O (0.1% HCl by weight) as the weak solvent. Liquid chromatography/mass spectrometry (LCMS) was performed on an Agilent 1290 UPLC-LCMS equipped with a C-18 silica column (1.8 μm, 2.1 × 50 mm) using CH₃CN/H₂O (0.1% acetic acid by volume): 5% to 95% CH₃CN over 4 min; 1 mL/min. The optical purity of the products was determined by derivatization with N-(5-fluoro-2,4-dinitrophenyl)alanamide (FDNP-alanamide)²⁰ as described below.

Boville C.E., Romney D.K., Almhjell P.J., Sieben M, & Arnold F.H. (2018). Improved synthesis of 4-cyanotryptophan and other tryptophan analogs in aqueous solvent using variants of TrpB from Thermotoga maritima. The Journal of organic chemistry, 83(14), 7447-7452.

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Analytical Characterization of Organic Compounds

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Nuclear magnetic resonance (NMR) spectra were recorded on a JEOL JNM-LA400 at 400 MHz for ¹H NMR and at 100 MHz for ¹³C NMR. Mass spectra (MS) were measured on a JEOL JMS-T100LP AccuTOF LC-plus 4G (electrospray ionization). Preparative high-performance liquid chromatography (HPLC) was performed on an Inertsil ODS-3 (10.0 × 250 mm) column (GL Sciences Inc.) using an HPLC system composed of a pump (PU-2080, JASCO) and a detector (MD-2015). Preparative medium pressure liquid chromatography (MPLC) was performed on an Isolera One purification system (Biotage) equipped with a Biotage SNAP Ultra C18 column (for reverse phase separation) or on an MPLC system composed of a pump and a detector (EPCLC-AI-580S, Yamazen) and equipped with a silica gel column (silica gel 40 μm or Amino 40 μm, Yamazen) (for normal phase separation). Liquid chromatography–mass spectrometry analysis was performed on an Acquity UPLC H-Class System (Waters) equipped with an Acquity UPLC BEH C18 1.7 μm (2.1 × 50 mm) column (Waters) and an MS detector (QDa, Waters).

Sakamoto S., Komatsu T., Watanabe R., Zhang Y., Inoue T., Kawaguchi M., Nakagawa H., Ueno T., Okusaka T., Honda K., Noji H, & Urano Y. (2020). Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples. Science Advances, 6(11), eaay0888.

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NMR, MS, and HPLC/MPLC Characterization

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NMR spectra were recorded using a JEOL JNM-LA400 spectrometer at 400 MHz for ¹H NMR and 100 MHz for ¹³C NMR. Mass spectra (MS) were obtained using JEOL JMS-T100LP AccuTOF LC-plus 4G (ESI).
Preparative HPLC was performed on an Inertsil ODS-3 (10.0 × 250 mm) column (GL Sciences Inc.) using an HPLC system composed of a pump (PU-2080, JASCO) and a detector (MD-2015). Preparative MPLC was performed on an Isolera One purification system (Biotage) equipped with a Biotage SNAP Ultra C18 column (for reverse phase separation) or on an MPLC system comprising a pump and detector (EPCLC AI-580S, Yamazen) and equipped with a silica gel column (silica gel 40 μm or Amino 40 μm, Yamazen) (for normal phase separation). LC-MS analysis was performed on an Acquity UPLC H-Class system (Waters) equipped with an Acquity UPLC BEH C18 1.7 μm (2.1 × 50 mm) column (Waters) and an MS detector (QDa or Xevo TQD, Waters).

Sakamoto S., Hiraide H., Minoda M., Iwakura N., Suzuki M., Ando J., Takahashi C., Takahashi I., Murai K., Kagami Y., Mizuno T., Koike T., Nara S., Morizane C., Hijioka S., Kashiro A., Honda K., Watanabe R., Urano Y, & Komatsu T. (2024). Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening. Cell Reports Methods, 4(1), 100688.

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