Mouse cystatin c duoset elisa kit

Urinary NGAL was quantified in 50 μL of a 1:5000 dilution of urine using the Mouse Lipocalin-2/NGAL DuoSet ELISA kit from R&D Systems (Minneapolis, MN, USA) following the manufacturer’s protocol. Serum Cystatin-C was quantified in 50 μL of 1:1000 dilutions of serum, using the Mouse Cystatin C DuoSet ELISA kit from R&D Systems (Minneapolis, MN, USA) following the manufacturer’s protocol. All samples were measured in duplicate. BUN was quantified in 50 μL of a 1:10 dilution of serum using the Urea Nitrogen (BUN) Colorimetric Detection Kit from Invitrogen (Frederick, MD, USA) following the manufacturer’s protocol. Serum creatinine was quantified in 15 μL of serum using the Creatinine (serum) Colorimetric Assay Kit from Cayman Chemicals (Ann Arbor, MI, USA) following the manufacturer’s protocol. Urinary albumin was quantified in 20–40 μL of urine; Exton solution (Cole-Parmer, Vernon Hills, IL, USA) was added to urine at a 1:1 ratio and samples were incubated for 10 min in the dark. Absorbance was read at 620 nm and values were multiplied by total volume of urine (mL) to determine albumin in mg/day.

Mouse Cystatin C DuoSet ELISA Kit (R&D Systems) was used to measure the protein level of cystatin c in serum according to the instruction supplied.