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Ethanol and dimethyl sulfoxide dmso

Manufactured by RCI Labscan
Sourced in Ireland

Ethanol and dimethyl sulfoxide (DMSO) are commonly used laboratory solvents. Ethanol is a clear, colorless, and flammable liquid with a characteristic odor. DMSO is a colorless, odorless, and polar aprotic solvent. Both solvents are widely used in various laboratory applications, including sample preparation, extraction, and reactions, but their specific intended uses are not included in this description.

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2 protocols using ethanol and dimethyl sulfoxide dmso

1

Colorimetric Assays for Antioxidant Activity

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Clostridium histolyticum collagenase (EC.3.4.23.3), porcine pancreatic elastase (PE–E.C.3.4.21.36), bovine testis hyaluronidase (E.C.3.2.1.3.5), N-[3-(2-furyl) acryloyl]-Leu-Gly-Pro-Ala (FALGPA), N-Succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), hyaluronic acid, tricine, sodium chloride, calcium chloride, 1,1-diphenyl-2-Picrylhydrazyl (DPPH), 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), potassium persulphate (K2S2O8), 2,4,6-tri (2-pyridyl)-s-triazine (TPTZ), sodium dihydrogen phosphate, disodium phosphate, sodium phosphate, sodium acetate, sodium thiocyanate, ferric chloride (FeCl3), Folin–Ciocalteu reagent, sodium carbonate, 3,4,5-trihydroxybenzoic acid (gallic acid), l-ascorbic acid (purity ≥ 99.0%), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), epigallocatechin-3-gallate (EGCG), ferrous sulfate, linoleic acid, oleanolic acid, lauryl glucoside (Plantacare® 1200), sorbitan Laurate (Span® 20), and polysorbate 20 (Tween® 20) were acquired from Sigma-Aldrich (Schnelldorf, Germany). Ethanol and dimethyl sulfoxide (DMSO), were analytical grade acquired from Labscan (Dublin, Ireland). Tris base, acetic acid, and hydrochloric acid were analytical grade acquired from Fisher Chem Alert (Fair Lawn, NJ, USA). HPLC grade methanol and acetonitrile was acquired from Carlo Erba Reagents (Cornaredo, Italy).
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2

Antioxidant and Enzymatic Assay Protocol

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Rutin, quercetin, Trolox, gallic acid, Folin–Ciocalteu reagent, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), collagenase from Clostridium histolyticum (ChC–EC.3.4.23.3), N-[3-(2-furyl) acryloyl]-Leu-Gly-Pro-Ala (FALGPA), elastase from porcine pancreatic (PE–E.C.3.4.21.36), N-Succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), hyaluronidase from bovine testis (E.C.3.2.1.3.5), sodium chloride (NaCl), calcium chloride (CaCl2), ferrous chloride (FeCl2), ferric chloride (FeCl3), 2,4,6 tripyridyl-s-triazine (TPTZ), glycerol, alcian blue 8GX, tricine, hyaluronic acid, sodium phosphate, and sodium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from GIBCO (Grand Island, NY, USA). Acetic acid, hydrochloric acid, and Tris base were purchased from Fisher Chem Alert (Fair Lawn, NJ, USA). Phosphoric acid and HPLC-grade acetonitrile were purchased from Merck (Darmstadt, Germany). Ethanol and dimethyl sulfoxide (DMSO) were analytical-grade and purchased from Labscan (Dublin, Ireland).
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