Vevo2100 echocardiography machine

Manufactured by Fujifilm

Sourced in Canada

The Vevo2100 is an echocardiography machine manufactured by Fujifilm. It is a non-invasive imaging device used for visualizing and evaluating the structure and function of the heart.

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Lab products found in correlation

Ms550d, by Fujifilm (5 mentions) Ms 550d probe, by Fujifilm (1 mentions) Model bx41, by Olympus (1 mentions) Ms 550d 40 mhz frequency probe, by Fujifilm (1 mentions)

7 protocols using vevo2100 echocardiography machine

Ventricular Function Monitoring via Echocardiography

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Ventricular function was monitored using 2D echocardiography on a weekly basis using a Visualsonics Vevo 2100 echocardiography machine and a MS 550D probe (22–55 MHz). Animals were anesthetized using isoflurane (0.75–1%). 2D echocardiography was performed at weekly intervals until the mice were ready to be sacrificed after 8 weeks of sham or TAC operation. Ejection fraction was determined from 2D images by manual determination of endocardium and epicardium from end-diastolic and end-systolic images.

Thai P.N., Daugherty D.J., Frederich B.J., Lu X., Deng W., Bers D.M., Dedkova E.N, & Schaefer S. (2018). Cardiac-specific Conditional Knockout of the 18-kDa Mitochondrial Translocator Protein Protects from Pressure Overload Induced Heart Failure. Scientific Reports, 8, 16213.

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Echocardiographic Evaluation of Murine Cardiac Function

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Echocardiographic image collection was performed using a Vevo2100 echocardiography machine (VisualSonics, Toronto, Canada) and a linear‐array 40 MHz transducer (MS‐550D). Image capture was performed in mice under general isoflurane anesthesia with heart rate maintained at 500–550 beats/min. LV systolic and diastolic measurements were captured in M‐mode from the parasternal short axis. Fraction shortening (FS) was assessed as follows: % FS = (end diastolic diameter ‐ end systolic diameter)/ (end diastolic diameter) x 100%. Left ventricular ejection fraction (EF) was measured and averaged in both the parasternal short axis (M‐Mode) using the tracing of the end diastolic dimension (EDD) and end systolic dimension (ESD) in the parasternal long axis: % EF = (EDD‐ESD)/EDD. Hearts were harvested at multiple endpoints depending on the study.

Wu J., Venkata Subbaiah K.C., Jiang F., Hedaya O., Mohan A., Yang T., Welle K., Ghaemmaghami S., Tang W.H., Small E., Yan C, & Yao P. (2020). MicroRNA‐574 regulates FAM210A expression and influences pathological cardiac remodeling. EMBO Molecular Medicine, 13(2), e12710.

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Myocardial Infarct Quantification via Histology and Echocardiography

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Hearts were harvested at the end of the study after perfusion and fixation in methanol/acetic acid fixative. Parasternal short axis section were cut before mounting and staining with Masson’s trichrome reagent. Slides were analyzed and photographed using an Olympus light microscope (Model BX41). The infarcted area (blue collagen staining for scar tissue) was expressed as percentage of LV surface area using ImageJ software (NIH). Echocardiography: Echocardiographic analysis using M-mode was performed using a Vevo2100 echocardiography machine (VisualSonics, Toronto, Canada) and a linear-array 40MHz transducer (MS-550D). LV systolic and diastolic measurements were captured in M-mode from the parasternal short axis. Heart function analysis completely described in supplemental information.

Cameron S.J., Ture S.K., Mickelsen D., Chakrabarti E., Modjeski K.L., McNitt S., Seaberry M., Field D.J., Le N.T., Abe J.I, & Morrell C.N. (2015). Platelet ERK5 is a Redox Switch and Triggers Maladaptive Platelet Responses and Myocardial Infarct Expansion. Circulation, 132(1), 47-58.

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Echocardiographic Phenotyping of Mouse TAC Model

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For the TAC surgery mouse model, M-mode short-axis echocardiographic image collection was performed using a Vevo2100 echocardiography machine (VisualSonics, Toronto, Canada) and a linear-array 40 MHz transducer (MS-550D). Heart rate was monitored during echocardiography measurement. Image capture was performed in mice under general isoflurane anesthesia with heart rate maintained at around 550–650 beats/min. The HR could vary in individual mice due to the potential effect of anesthesia or the surgeon’s operation variation. LV systolic and diastolic measurements were captured from the parasternal short axis in M-mode. Fraction shortening (FS) was assessed as follows: % FS = (end diastolic diameter - end-systolic diameter) / (end diastolic diameter) x 100%. Left ventricular ejection fraction (EF) was measured and averaged in both the parasternal short axis (M-Mode) using the tracing of the end-diastolic dimension (EDD) and end systolic dimension (ESD) in the parasternal long axis: % EF = (EDD-ESD)/EDD. Hearts were harvested at multiple endpoints depending on the study. In addition to EF and FS, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), and wall thickness of left ventricular anterior (LVAWT) and posterior (LVPWT) were also assessed.

Hedaya O.M., Venkata Subbaiah K.C., Jiang F., Xie L.H., Wu J., Khor E.S., Zhu M., Mathews D.H., Proschel C, & Yao P. (2023). Secondary structures that regulate mRNA translation provide insights for ASO-mediated modulation of cardiac hypertrophy. Nature Communications, 14, 6166.

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Angiotensin II and Doxorubicin Cardiac Effects in PDE1C-KO Mice

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Global PDE1C knockout (PDE1C-KO) mice were backcrossed to C57BL/6J mice for more than 10 generations. For experiments with genetically modified mice, age/sex/genetic background matched mice were randomly separated into indicated groups based on animal identification number. For the Ang II infusion model, PDE1C-KO and wild type (PDE1C-WT) mice from sibling mating at 10–12 weeks of age were subjected to subcutaneous infusion with vehicle saline or Ang II (1.4 mg/kg/day) for 28 days as described previously.^{16 (link)} For the doxorubicin cardiac toxicity model, PDE1C-WT, PDE1C-KO, or C57BL/6J male or female mice aged 10–12 weeks were randomly separated into indicated groups. Doxorubicin (20 mg/kg in saline) was administered via intra-peritoneal injection (i.p.) in a single bolus. IC86340 (6 mg/kg/day) and/or ZM241385 (10 mg/kg/day) in 20% DMSO/buffered saline were injected via i.p. two days prior to doxorubicin treatment and continued for additional five days. Mouse cardiac function was measured by echocardiography before sacrifice. Echocardiography was monitored in anesthetized mice using a Vevo2100 echocardiography machine equipped with an MS-550D 40 MHz frequency probe (VisualSonics, Toronto, Canada) as described previously in a blinded manner.^{15 (link)}

Zhang Y., Knight W., Chen S., Mohan A, & Yan C. (2018). A Multi-Protein Complex with TRPC, PDE1C, and A2R Plays a Critical Role in Regulating Cardiomyocyte cAMP and Survival. Circulation, 138(18), 1988-2002.

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Echocardiographic Evaluation of Cardiac Function in Murine Models

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For the sham and MI surgical mouse models, B-mode long-axis echocardiographic image collection was performed using a Vevo2100 echocardiography machine (VisualSonics, Toronto, ON, Canada) and a linear-array 40 MHz transducer (MS-550D, Fujifilm, Minato City, Tokyo). Heart rate was monitored during echocardiography measurement. Image capture was performed in mice under general isoflurane anesthesia with a heart rate maintained at around 550–650 beats/min. The HR could vary in individual mice due to the potential effect of anesthesia or the surgeon’s operation variation. LV systolic and diastolic measurements were captured in B-mode from the parasternal long axis. Fraction shortening (FS) was assessed as follows: %FS = (end diastolic diameter—end systolic diameter)/(end diastolic diameter) × 100%. Left ventricular ejection fraction (EF) was measured and averaged in both the parasternal short axis (M-Mode) using the tracing of the end diastolic dimension (EDD), and end systolic dimension (ESD) in the parasternal long axis: %EF = (EDD-ESD)/EDD. Hearts were harvested at multiple endpoints depending on the study. In addition to EF and FS, end systolic volume (LVESV) and end diastolic volume (LVEDV) were measured.

Subbaiah K.C., Wu J., Tang W.H, & Yao P. (2023). Ciclopirox Inhibition of eIF5A Hypusination Attenuates Fibroblast Activation and Cardiac Fibrosis. Journal of Cardiovascular Development and Disease, 10(2), 52.

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Echocardiographic Evaluation of Mouse TAC Model

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For the TAC surgery mouse model, M-mode short-axis echocardiographic image collection was performed using a Vevo2100 echocardiography machine (VisualSonics, Toronto, Canada) and a linear-array 40 MHz transducer (MS-550D). Heart rate was monitored during echocardiography measurement. Image capture was performed in mice under general isoflurane anesthesia with heart rate maintained at around 550-650 beats/min. The HR could vary in individual mice due to the potential effect of anesthesia or the surgeon’s operation variation. LV systolic and diastolic measurements were captured from the parasternal short axis in M-mode. Fraction shortening (FS) was assessed as follows: % FS = (end diastolic diameter - end systolic diameter) / (end diastolic diameter) x 100%. Left ventricular ejection fraction (EF) was measured and averaged in both the parasternal short axis (M-Mode) using the tracing of the end diastolic dimension (EDD) and end systolic dimension (ESD) in the parasternal long axis: % EF=(EDD-ESD)/EDD. Hearts were harvested at multiple endpoints depending on the study. In addition to EF and FS, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), and wall thickness of left ventricular anterior (LVAWT) and posterior (LVPWT) were also assessed.

Hedaya O.M., Subbaiah K.C., Jiang F., Xie L.H., Wu J., Khor E., Zhu M., Mathews D.H., Proschel C, & Yao P. (2023). Secondary structures that regulate mRNA translation provide insights for ASO-mediated modulation of cardiac hypertrophy. bioRxiv.

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