Neochlorogenic acid, gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, p-hydroxybenzoic acid, 1,3-dicaffeoylquinic acid, p-coumaric acid, rutin, quercetin 3-β-galactoside, ferulic acid, taxifolin, 3,4-dicaffeoylquinic acid, trans-m-coumaric acid, quercetin 3-α-L-rhamnoside, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, rosmarinic acid, myrcetin, luteolin, quercetin, trans-cinnamic acid, apigenin, kaempferol, fenofibrate, dimethyl sulfoxide (DMSO), ethylenediaminetetraacetic acid (EDTA), hematoxylin, eosin, and trizma base were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), streptomycin, and penicillin were purchased from Invitrogen (Carlsbad, CA, USA). Other reagents were commercially available and of special grade.
3 4 dicaffeoylquinic acid
Manufactured by Merck Group
Sourced in United States
3,4-dicaffeoylquinic acid is a naturally occurring compound found in various plant sources. It functions as an antioxidant and has been studied for its potential biological activities. This product is intended for research purposes only.
Automatically generated - may contain errors
Lab products found in correlation
3 5 dicaffeoylquinic acid, by Merck Group (3 mentions)
4 5 dicaffeoylquinic acid, by Merck Group (3 mentions)
Quercetin, by Merck Group (3 mentions)
Apigenin, by Merck Group (2 mentions)
Neochlorogenic acid, by Merck Group (2 mentions)
Chlorogenic acid, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Ferulic acid, by Merck Group (2 mentions)
Taxifolin, by Merck Group (1 mentions)
Myrcetin, by Merck Group (1 mentions)
Rosmarinic acid, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Luteolin, by Merck Group (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
Trans cinnamic acid, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
1 3 dicaffeoylquinic acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
4 protocols using 3 4 dicaffeoylquinic acid
1
Comprehensive Phytochemical Profiling
2
Standardized Antioxidant Compound Preparation
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Water was purified using a Milli–Q system (Millipore, Bedford, MA, USA). Ethanol (96%) was obtained from Vilniaus degtine (Vilnius, Lithuania). Anhydrous acetic acid (99.8%), and hydrochloric acid (37%) were purchased from Sigma-Aldrich (Buchs, Switzerland). The following reagents were used: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and diammonium salt (ABTS). The following standards were used: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), quercetin, chlorogenic acid, apigenin, 4-o-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, neochlorogenic acid, kaempferol-3-rutinoside, and ellagic acid from Sigma-Aldrich (Buchs, Switzerland) and santin from PlantMetaChem (Giesen, Germany). All the reagents and standards were of analytical grade. The stock solutions of phenolic compounds were prepared in 96% ethanol.
3
Quantification of Chlorogenic Acid Derivatives
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HPLC-grade standard substances, namely neochlorogenic acid (5-caffeoylquinic acid), chlorogenic acid (3-caffeoylquinic acid), cryptochlorogenic acid (4-dicaffeoylquinic acid), 4,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were purchased from Sigma–Aldrich GmbH (Steinheim, Germany). Solvents and other substances were obtained as follows: acetonitrile (purity 99.9%) and methanol (purity 99.9%) from Sigma-Aldrich (Steinheim, Germany); trifluoroacetic acid (purity 99.8%) supplied from Merck (Darmstadt, Germany); 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) (purity ≥ 98%) and potassium persulphate (purity ≥ 99%) purchased from Sigma (St. Louis, MO, USA); Trolox (purity ≥ 98%) received from Fluka Chemika (Buchs, Switzerland). Ultrapure water was purified using a Millipore water-cleaning system (Bedford, MA, USA).
4
Analytical Standards for Bioactive Compounds
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Analytical-grade hexane was purchased from Poch (Gliwice, Poland); HPLC-grade formic acid and acetonitrile from Fluka (St. Louis, MO, USA); 5-caffeoylquinic acid (99%), caffeic acid (99%), ferulic acid (99%), 3,4-dicaffeoylquinic acid (98%), benzoic acid (99%), catechin (≥ 99%), epicatechin (≥ 98%), epigallocatechin (≥ 90%), procyanidin B2 (≥ 90%), procyanidin C1 (≥ 75%), quercetin (≥ 95%), quercetin-3-O-glucoside (≥ 98%), quercetin-3-O-galactoside (≥ 97%), and quercetin-3-O-arabinoside (≥ 95%) from Sigma-Aldrich (St. Louis, MO, USA); 3-caffeoylqunic acid (99%), 4-caffeoylqunic acid (99%), 3,5-dicaffeoylquinic acid (99%), and 4,5-dicaffeoylquinic acid (99%) from Phy-toLab (Vestenbergsgreuth, Germany); and nylon filters from Chromacol (Herts, UK). Ultrapure water (resistivity, 18.2 MΩ cm) was obtained from a Millipore Milli-Q Plus purification system (Bedford, MA, USA). Kits for serum marker enzyme activities were from LaChema (Brno, Czech Republic); kits for TNF-α, TGF-β, IL-6, and IFN-γ determination from R&D Systems GmbH (Wiesbaden, Germany); kits for hyaluronic acid, laminin, type I collagen measurements, and assay kits for measurement of hyaluronic acid, laminin, and type I collagen from Wuhan USCN Sciences Co., Ltd. (Wuhan, China).
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