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Annexin 5 fitc and pi kit

Manufactured by Thermo Fisher Scientific
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The Annexin V FITC and PI kit is a laboratory reagent designed to detect and quantify apoptosis in cells. It contains Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a DNA-binding dye. This kit allows for the identification of early and late apoptotic cells through flow cytometry analysis.

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Lab products found in correlation

Lsr18 machine, by FlowJo (3 mentions) Fxcycle pi rnase staining solution, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions) Propidium iodide, by Merck Group (1 mentions) Canto flow cytometer, by BD (1 mentions) Rnase a, by Merck Group (1 mentions)

Close spelling variants of this product

Dead Cell Apoptosis Kit with Annexin V-fluorescein (FITC) and PI (V13242), Dead Cell Apoptosis Kit with Annexin V FITC and PI Annexin V-FITC and PI apoptosis detection kit Annexin-V FITC and PI double staining kit Annexin V-FITC and PI apoptosis kit Dead Cell Apoptosis Kit with annexin V-FITC and PI for flow cytometry FITC Annexin V/Dead Cell Apoptosis Kit with FITC annexin V and PI Annexin V-FITC and PI Annexin V-FITC/PI Annexin V and PI

6 protocols using annexin 5 fitc and pi kit

1

Annexin V-FITC and TMRE Assay

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Phosphatidylserine externalization was detected using an Annexin V FITC and PI kit (Thermo Fisher, Cat. No. V13242) following the manufacturer’s instructions. Briefly, cells were washed with cold PBS and resuspended in Annexin V binding buffer and stained with Annexin V and PI at room temperature and then analyzed immediately. To measure change in mitochondria membrane potential cells were treated with 200nM of tetramethylrhodamine, ethyl ester (TMRE; Abcam, Cat. No. ab113852) for 15min at 37 °C. Annexin V and TMRE-positive cells were detected using the Becton-Dickinson LSR18 machine and analysed with FlowJo version 7 software module.
Bitler B.G., Wu S., Park P.H., Hai Y., Aird K.M., Wang Y., Zhai Y., Kossenkov A.V., Vara-Ailor A., Rauscher FJ I.I.I., Zou W., Speicher D.W., Huntsman D.G., Conejo-Garcia J.R., Cho K.R., Christianson D.W, & Zhang R. (2017). ARID1A-mutated ovarian cancers depend on HDAC6 activity. Nature cell biology, 19(8), 962-973.
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2

Apoptosis and Cell Cycle Analysis

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Apoptotic cells were detected using Annexin-V-FITC and PI kit (Thermo Fisher Scientific, Waltman, MA, USA) following the manufacturer's instructions, and presented as the percentage (%) of Annexin-V+PI+ cells.
To determine cell-cycle distribution, cells were collected, washed with PBS three times, and fixed in 70% ethanol overnight. Fixed cells were then stained using FxCycle™ PI/RNase Staining Solution (Thermo Fisher Scientific) according to the manufacturer's instructions. All FACS analysis was performed on FACSCalibur (BD Biosciences, San Jose, CA, USA).
Lv H.W., Xing W.Q., Ba Y.F., Li H.M., Wang H.R, & Li Y. (2021). SMYD3 confers cisplatin chemoresistance of NSCLC cells in an ANKHD1-dependent manner. Translational Oncology, 14(6), 101075.
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3

Quantifying Apoptotic Cell Populations

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Apoptotic cells were detected using Annexin-V-FITC and PI kit (Thermo Fisher Scientific) following the manufacturer’s instructions. The percentage (%) of apoptotic cells was calculated as the  % sum of Annexin-V+PI− (representing early apoptotic cells) and Annexin-V+PI+ cells (representing late apoptotic cells).
Gao G., Tian Z., Zhu H.Y, & Ouyang X.Y. (2018). miRNA-133b targets FGFR1 and presents multiple tumor suppressor activities in osteosarcoma. Cancer Cell International, 18, 210.
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4

Annexin V FITC and PI Cytometry Assay

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The Annexin V FITC and PI kit (Thermo Fisher, Cat. No. V13242) was used following the manufacturer’s instructions. Briefly, cells were washed by PBS, trypsinized, and suspended in annexin V binding buffer. Cells were stained with annexin V and PI for 15 min and then analyzed. Analysis was performed using FlowJo version 7 software module.
For cell cycle analysis, the cells were fixed, treated with bovine RNase, and then stained with propidium iodide (Sigma-Aldrich) for 15 minutes at room temperature. Samples were subjected to analysis using the Becton-Dickinson LSR18 machine and FlowJo version 7 software module.
Fukumoto T., Zhu H., Nacarelli T., Karakashev S., Fatkhutdinov N., Wu S., Liu P., Kossenkov A.V., Showe L.C., Jean S., Zhang L, & Zhang R. (2019). N6-methylation of adenosine (m6A) of FZD10 mRNA contributes to PARP inhibitor resistance. Cancer research, 79(11), 2812-2820.
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5

Annexin V-FITC and TMRE Assay

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Phosphatidylserine externalization was detected using an Annexin V FITC and PI kit (Thermo Fisher, Cat. No. V13242) following the manufacturer’s instructions. Briefly, cells were washed with cold PBS and resuspended in Annexin V binding buffer and stained with Annexin V and PI at room temperature and then analyzed immediately. To measure change in mitochondria membrane potential cells were treated with 200nM of tetramethylrhodamine, ethyl ester (TMRE; Abcam, Cat. No. ab113852) for 15min at 37 °C. Annexin V and TMRE-positive cells were detected using the Becton-Dickinson LSR18 machine and analysed with FlowJo version 7 software module.
Bitler B.G., Wu S., Park P.H., Hai Y., Aird K.M., Wang Y., Zhai Y., Kossenkov A.V., Vara-Ailor A., Rauscher FJ I.I.I., Zou W., Speicher D.W., Huntsman D.G., Conejo-Garcia J.R., Cho K.R., Christianson D.W, & Zhang R. (2017). ARID1A-mutated ovarian cancers depend on HDAC6 activity. Nature cell biology, 19(8), 962-973.
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6

Apoptosis and Cell Cycle Analysis

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Cells were treated with indicated doses of SCH772984 and phenformin for 48 hours. Analysis of apoptotic cells by annexin V staining (Annexin V FITC and PI kit, Thermo Scientific) was done according to manufacturer's instructions. Cell cycle analysis was performed after fixation in 70% ethanol and incubation with PBS containing 0.5 μg/mL RNaseA (Sigma-Aldrich) and 50 μg/mL propidium iodide (PI; Sigma-Aldrich, St. Louis, MO). Data was acquired on a Canto Flow Cytometer (Becton Dickinson, Franklin Lakes, NJ) and analyzed using FlowJo software (Treestar, Ashland, OR).
Trousil S., Chen S., Mu C., Shaw F.M., Yao Z., Ran Y., Shakuntala T., Merghoub T., Manstein D., Rosen N., Cantley L.C., Zippin J.H, & Zheng B. (2017). Phenformin enhances the efficacy of ERK inhibition in NF1-mutant melanoma. The Journal of investigative dermatology, 137(5), 1135-1143.
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