Capillary cell cuvette

Samples were prepared by adding 20μL of nanoparticle stock solution (1mg/mL) in the 980μL of different dispersants namely 1) HPLC grade water, 2) DMEM with fetal bovine serum (FBS), 3) DMEM without FBS and 4) PBS. Afterwards, the samples were analyzed at a volume of 1 mL in a capillary cell cuvette (Malvern) on a Malvern Zetasizer Nano Range Dynamic Light Scattering instrument.

The sizes, polydispersity index (PDI), and zeta potentials of 5, 10, and 80 nm AuNPs coated with citrate at a concentration of 10 µg/mL were measured in Ultrapure Water (UPW), and cell culture media (CCM) supplemented with 10% FBS and 1% penicillin/streptomycin at 0 and 24 h by dynamic light scattering (DLS) (Malvern Instruments Ltd., Worcestershire, UK) at 25 °C. A total of 1 mL of the dispersed AuNPs were pipetted into a disposable polystyrene cuvette (Bohemia, NC, USA) for size and PDI measurement. For zeta potential, the dispersions were pipetted into a capillary cell cuvette (Malvern Instruments Ltd., Worcestershire, UK). The morphology and size distribution of the nanoparticles was studied using a transmission electron microscope (TEM) (JEOL Ltd. Hertfordshire, UK) operating at an accelerating voltage of 80 keV (JEOL 1400 TEM, University of Birmingham, Centre for Electron Microscopy). A freshly prepared 10 µL of AuNPs (10 µg/mL) dispersed in UPW was deposited onto a copper 300 Mesh TEM grid (EM Resolutions Ltd., Sheffield, UK) and allowed to dry for 2 h in a safety cabinet before observation by TEM.

The hydrodynamic diameter, polydispersity
index (PDI), and zeta potential of 5 nm functionalized AuNPs (Tannate,
citrate, and PVP) at a concentration of 10 μg/mL were measured
in ultrapure water (UPW), phosphate buffer saline (PBS), free culture
media (FCM), and cell culture media (CCM) supplemented with 10% FBS
and 1% penicillin/streptomycin using dynamic light scattering (DLS)
(Malvern Instruments Ltd., Worcestershire, UK) at 25 °C. A total
of 1 mL of the dispersed AuNPs was pipetted into a disposable polystyrene
cuvette (Bohemia, NC, USA) for size and PDI measurement. For zeta
potential, the dispersions were pipetted into a capillary cell cuvette
(Malvern Instruments Ltd., Worcestershire, UK). The morphology and
size distribution of the nanoparticles were studied using Transmission
Electron Microscopy (TEM) (JEOL Ltd., UK) instrument operating at
an accelerating voltage of 80 keV (JEOL 1400 TEM, University of Birmingham,
Centre for Electron Microscopy). A freshly prepared 10 μL portion
of AuNPs (10 μg/mL) dispersed in UPW was deposited onto a copper
300 Mesh TEM grid (EM Resolutions Ltd., Sheffield, UK) and allowed
to dry for 2 h in a safety cabinet before observation by TEM.