Log In Sign up
The largest database of trusted experimental protocols

Ccr6 quantitect primers

Manufactured by Qiagen
Visit Supplier
Sourced in Canada

The CCR6 Quantitect primers are a set of oligonucleotide sequences designed for the quantitative detection and analysis of the CCR6 gene expression using real-time PCR technology. The primers target a specific region of the CCR6 gene and are optimized for use with the QuantiTect Primer Assay system.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy kit, by Qiagen (2 mentions) Mastercycler pcr system, by Eppendorf (1 mentions)

Spelling variants of this product

QuantiTect primers (247 citations) QuantiTect (227 citations)

2 protocols using ccr6 quantitect primers

1

RNA Extraction and Quantitative RT-PCR

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNeasy kit (Qiagen Inc., Hilden, Germany), as previously described [45 (link),47 (link)]. One-step reverse transcription PCR (RT-PCR) (Qiagen Inc., Toronto Ontario, Canada) using CCR6 Quantitect primers (Qiagen Inc., Toronto) was carried out in an Eppendorf Mastercycler PCR System (Applied Biosystems, Foster City, California, USA). The products of RT-PCR amplifications were visualized on a 1% agarose gel. Reactions were performed in the presence and absence of reverse transcriptase to distinguish between genomic DNA and mRNA amplification.
Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.
+ Open protocol
+ Expand
2

RNA Extraction and RT-PCR for CCR6

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNeasy kit (Qiagen), as previously described [45 (link), 47 (link)]. One step RT-PCR (Qiagen) using CCR6 Quantitect primers (Qiagen) was carried out in an Eppendorf Mastercycler PCR System. The products of RT-PCR amplifications were visualized on a 1% agarose gel. Reactions were performed in the presence and absence of reverse transcriptase to distinguish between genomic DNA and mRNA amplification.
Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!