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Lumox dishes

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Lumox dishes are a type of cell culture vessel manufactured by Sarstedt. They are designed for the cultivation and analysis of cells in a laboratory setting.

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Lab products found in correlation

Bone morphogenetic protein 4 (bmp4), by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) B 27 supplement without vitamin a, by Thermo Fisher Scientific (1 mentions) Primocin, by InvivoGen (1 mentions) Neurobasal medium, by Thermo Fisher Scientific (1 mentions) Sb431542, by Bio-Techne (1 mentions) Y 27632, by Bio-Techne (1 mentions) Ultraview vox, by PerkinElmer (1 mentions) Tcs sp5 2 confocal microscope, by Leica (1 mentions)

Close spelling variants of this product

Lumox dish Lumox-bottom dish Lumox teflon-bottom dish Lumox dish 35 Lumox dish 50 Lumox cell culture dishes Lumox

5 protocols using lumox dishes

1

Cerebral and Basal Organoid Formation

Cited 2 times
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Cerebral and basal GE organoid formation was performed as described in Watanabe et al. (2017) (link). Of note, KSR is known to affect differentiation efficiency, and we carried out the differentiation protocol using the same KSR lots (Invitrogen, KSR 10828010 and 10828028; lot 1670543) as in Watanabe et al. (2017) (link). For the formation of dorsomedial telencephalic (DMT) organoids, we used a modified version of previously described methods (Sakaguchi et al., 2015 (link)). From day 18 to day 24, 6.5 ng/mL BMP4 (Invitrogen, P2026055) and 3 μM GSK3 inhibitor (CHIR 99021; Fisher Scientific, 442310) were added with DMEM/F-12 (Hyclone) supplemented with 1% N2 (Invitrogen), 1% chemically defined lipid concentrate (CDLC; Invitrogen), and 10% fetal bovine serum (FBS qualified source US region; Invitrogen, 10437028) under 40% O2 and 5% CO2 conditions at 37°C. On day 42, DMT organoids were cut in half and the base medium was changed to Neurobasal medium (Invitrogen), supplemented with 1% N2 (Invitrogen), 1% CDLC, 10% FBS, 2% B-27 supplement without vitamin A (Invitrogen), GlutaMAX (Invitrogen), and 100 μg/mL Primocin (InvivoGen). After day 42, DMT organoids were cut in half every 2 weeks. From day 56, DMT organoids were cultured in Lumox dishes (Sarstedt). Medium was subsequently changed every 2–3 days until the organoids were collected for analysis.
Watanabe M., Buth J.E., Haney J.R., Vishlaghi N., Turcios F., Elahi L.S., Gu W., Pearson C.A., Kurdian A., Baliaouri N.V., Collier A.J., Miranda O.A., Dunn N., Chen D., Sabri S., Torre-Ubieta L.D., Clark A.T., Plath K., Christofk H.R., Kornblum H.I., Gandal M.J, & Novitch B.G. (2022). TGFβ superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids. Stem Cell Reports, 17(10), 2220-2238.
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2

Generation of Cerebral Organoids from iPSCs

Cited 1 time
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We generated cerebral organoids according to previously published protocols (Kadoshima et al., 2013 (link)); (Bershteyn et al., 2017 (link)). Human and chimpanzee iPSCs were dissociated to single cells with Accutase and re-aggregated in lipidure-coated 96-well V-bottom plates at a density of 10,000 cells per aggregate, in 100 μL of cortical differentiation medium per well. The cortical differentiation medium (Glasgow-MEM, 20% KSR, 0.1mM NEAA, 1mM sodium pyruvate, 0.1mM β-ME, 100 U/mL penicillin/streptomycin) was supplemented with Rho Kinase Inhibitor (Y-27632, 20 μM, Tocris, Cat# 1254 day 0 and day 3), WNT inhibitor (IWR1-ε, 3 μM, Cayman Chemical Cat# 13659 days 0–18) and TGF-β inhibitor (SB431542, Tocris, Cat #1614, 5 μM, days 0–18). Media was changed on days 3 and 6 and then every 2–3 days until day 18. Aggregates were then transferred to ultra low adhesion 6-well plates in DMEM/F12 medium with Glutamax supplemented with N2, Lipid Concentrate, Fungizone (2.5 μg/mL), and penicillin/streptomycin (100 U/mL) and grown under 40% O2 5% CO2 conditions. After five weeks, FBS (10% v/v), Matrigel (1% v/v) and heparin (5 μg/mL) were added to the medium, and after 8 weeks organoids were transferred to lumox dishes (Sarstedt), which have a gas permeable base.
Pollen A.A., Bhaduri A., Andrews M.G., Nowakowski T.J., Meyerson O.S., Mostajo-Radji M.A., Di Lullo E., Alvarado B., Bedolli M., Dougherty M.L., Fiddes I.T., Kronenberg Z.N., Shuga J., Leyrat A.A., West J.A., Bershteyn M., Lowe C.B., Pavlovic B.J., Salama S.R., Haussler D., Eichler E.E, & Kriegstein A.R. (2019). Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution. Cell, 176(4), 743-756.e17.
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3

Imaging Macrophage Fascin Bundles

Cited 3 times
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UAS transgenic constructs encoding fluorescent proteins were expressed specifically in macrophages using the singed-Gal4 driver line (Zanet et al., 2012 (link)). Live embryos were mounted as previously described (Wood et al., 2006 (link)). Briefly, stage 15 embryos were dechorionated in bleach and mounted under coverslips on hydrophobic Lumox dishes (Sarstedt, Germany) in Voltalef oil 10S. Embryos were then imaged with a spinning disk microscope (UltraVIEW VoX PerkinElmer) with a 63× N.A. 1.4 objective acquiring one image every 30 s. Measurements of the length of fascin bundles were performed using Volocity software. MT arms were defined as polarised bundles of MT that anticipate direction of migration as previously described previously (Stramer et al., 2010 (link)).
Villari G., Jayo A., Zanet J., Fitch B., Serrels B., Frame M., Stramer B.M., Goult B.T, & Parsons M. (2015). A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration. Journal of Cell Science, 128(24), 4601-4614.
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4

Fluorescence microscopy

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For confocal microscopy cells were grown in lumox dishes (Sarstedt, Leichester, UK, cat. no. 94.6077.331). After incubation with 1 μM thapsigargin cells were incubated with 2 μg/ml JC-1 (Life Technologies, cat. no. T3168) at 37°C for 20 min (Ankarcrona et al., 1995 (link); Cossarizza et al., 1993 (link); Reers et al., 1991 (link); Smiley et al., 1991 (link)). The cells were washed twice with PBS before addition of fresh medium for live cell imaging on a Leica TCS SP5 II confocal microscope (Leica Microsystems, Mannheim, Germany). JC-1 fluorescence was excited at 488 nm with an argon laser set at 22% of its maximum power. Green fluorescence between 515-545 nm was collected with a photomultiplier tube and orange fluorescence between 590-620 nm with a HyD 5 detector. Cells showing fluorescence emission between 515-545 nm only were counted as having undergone MPT, while cells that displayed punctuate fluorescence emission between 590-620 nm were counted as not having undergone MPT.
Brown M., Strudwick N., Suwara M., Sutcliffe L.K., Mihai A.D., Ali A.A., Watson J.N, & Schröder M. (2016). An initial phase of JNK activation inhibits cell death early in the endoplasmic reticulum stress response. Journal of cell science, 129(12), 2317-2328.
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5

Radiobiological Characterization of U87 Glioblastoma

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U87 Glioblastoma cells were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin. 35 mm lumox® dishes (Sarstedt AG & Co., Nümbrecht, Germany) were modified to hold a 1.4 μm mylar film bottom, required for sufficient penetration of the alpha particles into the cell layer. The modified dishes were irradiated to 10 kGy from a Co-60 source for sterilization. U87 cells in culture flasks were resuspended using trypsin, pipetted repeatedly to avoid cell clumps and counted using a digital cell counter, after which 60.000 cells were transferred to mylar dishes. The cells were allowed to settle overnight and irradiated to 0, 125, 249, 498, 747 and 996 mGy (± 5.3%) [23 ]. To minimize stress induced pH changes following atmospheric exposure, cells were irradiated in medium with 20 mM HEPES and each mylar dish was exposed to ambient air for an equal amount of time. After irradiation, 1900 – 5000 cells per well, depending on the expected survival, were transferred from the mylar dish onto 3 wells in a 24-well plate. The 24-well plates were incubated for 7 days after which the number of surviving cells were counted using the SRB assay [24 ]. The procedure was performed twice to improve reliability, which was deemed sufficient for the proof-of-principle of this work.
Kouwenberg J.J., Wolterbeek H.T., Denkova A.G, & Bos A.J. (2018). Fluorescent nuclear track detectors for alpha radiation microdosimetry. Radiation Oncology (London, England), 13, 107.
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