RNA was extracted using the RNeasy Mini Kit for total RNA (Qiagen, GmBH, Cat. No. 74104), according to the manufacturer’s protocol. The concentration and quality of the extracted RNA were assessed using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). The integrity of RNA was verified by the Agilent Bioanalyzer 2100 (Agilent Technologies, Inc.). Reverse transcription of 2 µg of the extracted RNA was carried out using the High-Capacity c-DNA Reverse Transcription Kit (Applied Biosystem, ThermoFisher Scientific, USA, Cat. No. k1691), according to the manufacturer’s protocol. Reverse transcription was performed in a 20 µl reaction volume at 25 °C for 10 min, 37 °C for 120 min, and finally, heat inactivation at 85 °C for 5 min.
Rneasy mini kit for total rna
Manufactured by Qiagen
The RNeasy Mini Kit is a laboratory equipment designed for the isolation and purification of total RNA from a variety of biological samples. The kit utilizes a specialized silica-based membrane to selectively bind RNA molecules, allowing for efficient separation and recovery of high-quality RNA for downstream applications.
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Lab products found in correlation
2 protocols using rneasy mini kit for total rna
1
RNA Extraction and cDNA Synthesis
2
Extraction and Purification of RNA
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Total RNAs were extracted and purified from 7 pairs of specimens for lncRNA, mRNA and miRNA, respectively, using an RNeasy mini kit for total RNA (Qiagen, GmBH) and mirVana™ miRNA Isolation kit without phenol (Ambion; Thermo Fisher Scientific, Inc.). The whole process was conducted according to the manufacturer's protocols. NanoDrop ND-2000 (Thermo Fisher Scientific, Inc.) was applied for quantification of total RNAs and RNA Integrity Number was detected using Agilent Bioanalyzer 2100 (Agilent technologies, Inc.).
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