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Anti cd64 apc vio770

Manufactured by Miltenyi Biotec
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Sourced in Germany, United States

The Anti-CD64-APC-Vio770 is a flow cytometry reagent that detects the expression of CD64 (Fc gamma RI) on the surface of cells. CD64 is a high-affinity Fc receptor that binds the Fc portion of IgG antibodies. This reagent can be used to identify cells expressing CD64, which is important in various immune and inflammatory processes.

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Lab products found in correlation

Propidium iodide viability dye, by Miltenyi Biotec (2 mentions) Anti cd11b apc, by Miltenyi Biotec (2 mentions) Anti ly6c fitc, by Miltenyi Biotec (2 mentions) Anti ly6g vioblue, by Miltenyi Biotec (2 mentions) Anti cd11c pe vio770, by Miltenyi Biotec (2 mentions) Anti b220 vioblue, by Miltenyi Biotec (2 mentions) Anti mhc 2 fitc, by Miltenyi Biotec (2 mentions) Fcr block, by Miltenyi Biotec (2 mentions) Macsquant system, by Miltenyi Biotec (1 mentions) Complete rpmi medium, by Thermo Fisher Scientific (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) L glutamine, by Merck Group (1 mentions) Calf serum, by Merck Group (1 mentions) Macsquantify software, by Miltenyi Biotec (1 mentions) Macrophage colony stimulating factor m csf, by Miltenyi Biotec (1 mentions) Anti cd163 apc, by Miltenyi Biotec (1 mentions) Anti cd206 pe, by Thermo Fisher Scientific (1 mentions) Anti f4 80 pe, by Miltenyi Biotec (1 mentions)

3 protocols using anti cd64 apc vio770

1

Multiparametric Flow Cytometry of Immune Cells

Cited 2 times
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Cervical lymph node cells were isolated, washed, counted, and resuspended at 100,000 cells in 50 μL of flow cytometry buffer. Cells were treated with FcR-block (Miltenyi Biotec, Bergisch Gladbach, Germany) and labeled for surface markers via antibodies, per the manufacturer’s instructions31 (link),39 (link). Dead cells were excluded from analysis by labeling with propidium iodide viability dye (Miltenyi Biotec), per the manufacturer’s instructions31 (link),39 (link). Neutrophils: anti-CD11b-APC (Miltenyi Biotec; clone REA592), anti-Ly6C-FITC (Miltenyi Biotec; clone REA796), anti-Ly6G-VioBlue (Miltenyi Biotec; clone 1A8). M1-macrophages: anti-CD11b-APC (Miltenyi Biotec; clone REA592), anti-MHC II-FITC (Miltenyi Biotec; clone REA528), anti-CD206-PE (Miltenyi Biotec; clone MR6F3), anti-CD64-APC-Vio770 (Miltenyi Biotec; clone REA286). Plasmacytoid dendritic cells: anti-CD11c-PE-Vio770 (Miltenyi Biotec; clone REA754), anti-MHC II-FITC (Miltenyi Biotec; clone REA528), anti-B220-VioBlue (Miltenyi Biotec; clone REA755). A minimum of 5,000 gated cells were analyzed per specimen. Data was acquired by the MACSQuant System (Miltenyi Biotec) and analyses were performed via FlowJo 11.0 software (TreeStar, Ashland, OR, USA), as previously reported31 (link),39 (link).
Swanson B.A., Carson M.D., Hathaway-Schrader J.D., Warner A.J., Kirkpatrick J.E., Corker A., Alekseyenko A.V., Westwater C., Aguirre J.I, & Novince C.M. (2021). Antimicrobial-Induced Oral Dysbiosis Exacerbates Naturally Occurring Alveolar Bone Loss. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e22015.
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2

In Vitro Macrophage Polarization Protocol

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For in vitro macrophage induction, purified CD14+ monocytes were cultured in a complete RPMI medium (Life Technologies, Bleiswijk, The Netherlands) supplemented with 10% heat-inactivated calf serum (Merck Life Science, Madrid, Spain), 2 mM L-glutamine (Merck Life Science, Madrid, Spain), 1% antibiotic/antimycotic solution, and 100 ng/mL Macrophage Colony-Stimulating Factor (M-CSF) (Miltenyi Biotec, Bergisch Gladbach, Germany) for 8 days. Afterwards, M0 macrophages were polarized towards a M1 phenotype by incubating them with 120 ng/mL of IFNγ and 10 ng/mL of Lipopolysaccharides (LPS) (Merck Life Science, Madrid, Spain) for 48 h. For M2 phenotype, M0 macrophages were incubated with 2 µg/mL IL-4 (Peprotech, London, UK) for 48 h. MSCs were co-cultured with either M0, M1, or M2 macrophages at a ratio of 1:1. After 2 days macrophages were stained with the following antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany): anti-CD163-APC, anti-CD64-APC-Vio770, anti-CD209-PE-Vio770, anti-CD86-FITC, anti-MHC-I- PeVio770, anti-CD206 VioBlue, or with the corresponding isotype control antibodies. Data were acquired by flow cytometry using the MACSQuant® analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany). Data were analyzed using MACSQuantify software (Miltenyi Biotec, Bergisch Gladbach, Germany) and FlowJoTM v10.6.2 Software (BD Life Sciences, Ashland, OR, USA).
Hernaez-Estrada B., Gonzalez-Pujana A., Cuevas A., Izeta A., Spiller K.L., Igartua M., Santos-Vizcaino E, & Hernandez R.M. (2022). Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation. Biomedicines, 10(2), 253.
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3

Immune Cell Subset Analysis Protocol

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Cells were treated with FcR‐block (Miltenyi Biotec) and stained for cell surface markers. Myeloid‐derived suppressor cells (MDSCs): anti‐CD11b‐APC (Miltenyi Biotec; clone REA592), anti‐Ly6G‐VioBlue (Miltenyi Biotec; clone 1A8), anti‐F4/80‐PE (Miltenyi Biotec; clone REA126), anti‐Ly6C‐FITC (Miltenyi Biotec; clone REA796). Plasmacytoid dendritic cells (pDC): anti‐CD11c‐PE‐Vio770 (Miltenyi Biotec; clone REA754), anti‐MHC II‐FITC (Miltenyi Biotec; clone REA528), anti‐B220‐VioBlue (Miltenyi Biotec; clone REA755). M1/M2 macrophages: anti‐CD11b‐APC (Miltenyi Biotec; clone REA592), anti‐CD11c‐PE‐Vio770 (Miltenyi Biotec; clone REA754), anti‐MHC class II‐FITC (Miltenyi Biotec; clone REA528), anti‐CD64‐APC‐Vio770 (Miltenyi Biotec; clone REA286), anti‐B220‐VioBlue (Miltenyi Biotec; clone REA755), anti‐CD206‐PE (eBioscience, Santa Clara, CA, USA; clone MR6F3). Dead cells were excluded from analysis via propidium iodide viability dye (Miltenyi Biotec).
Hathaway‐Schrader J.D., Poulides N.A., Carson M.D., Kirkpatrick J.E., Warner A.J., Swanson B.A., Taylor E.V., Chew M.E., Reddy S.V., Liu B., Westwater C, & Novince C.M. (2020). Specific Commensal Bacterium Critically Regulates Gut Microbiota Osteoimmunomodulatory Actions During Normal Postpubertal Skeletal Growth and Maturation. JBMR Plus, 4(3), e10338.
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