Nematodes containing rhodamine-labeled pSi particles were visualized on an Olympus IX70 Delta Vision microscope (Applied Precision, Issaquah, WA, USA) using either Nikon 10× or 40× objective lens and photographed with a CoolSnap HQ2 CCD camera (PhotoMetrics, Inc., USA). Non-fluorescent images were captured using differential interference contrast (DIC); fluorescent images were deconvolved using the SoftWorx program (Applied Precision). An RD-TR-PE filter (Ex/Em 535/617 nm) was used for fluorescence image acquisition. To prepare specimens for microscopy, 8–10 C. elegans or 2 adult A. ceylanicum organisms were mounted on 2% agarose pads containing 10 mM sodium azide; a coverslip was overlaid to fix the position of the worms. Images were obtained within 2 h of slide preparation to avoid drying of the agarose pads.
Softworx program
Manufactured by Cytiva
SoftWorx is a software program developed by Cytiva to control and manage laboratory equipment. It provides a user-friendly interface for operating and monitoring various instruments used in scientific research and analysis.
Automatically generated - may contain errors
2 protocols using softworx program
1
Visualization of Rhodamine-Labeled Particles in Nematodes
2
Microscopy and Cell Cycle Analysis
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Images for colony morphology were acquired with G:BOX imaging system (Syngene). Florescence microscopy was performed essentially as described previously [44 (link)]. Images were acquired on a DeltaVision Microscopy System (Applied Precision, LLC) using an inverted microscope (TE200; Nikon), a charge-coupled device camera (CoolSNAP HQ; Roper Scientific) and either a 40× objective with a 0.75 NA or a 20× with a 0.50 NA (Nikon). Figures were prepared for publication using Adobe Photoshop and Adobe Illustrator. No further manipulations other than adjustments in brightness and contrast were made. To determine DNA contents, cells were grown to exponential phase, fixed with 70% ethanol, and treated with RNAase. The cells were then stained with propidium iodide overnight at 4°C and fluorescent intensity was quantified with CellProfiler image analysis software [45 (link)]. For time-lapse experiments, cells were grown to early exponential phase in liquid YPD medium and were placed on YPD agarose pads. 10 z sections spaced 1 μm apart without binning were acquired every 2 min. All images were projected and processed with the softWoRx program (Applied Precision, LLC).
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