E10.5 embryos were fixed in 4% paraformaldehyde in PBS at 4°C, rinsed in PBS, then permeabilized in PBS with 0.5% Triton X-100 for 24 h at 4°C. Neurofilament immunodetection was performed as previously described (Ray et al., 2020 (link)). Primary anti-neurofilament antibody (Developmental Studies Hybridoma Bank, 2H3) was used at a 1:20 dilution, and anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (Jackson ImmunoResearch, 115-035-003) was used at 1:1000 dilution. Signal was developed using an ImmPACT DAB Substrate Kit (Vector Laboratories, SK-4105). Photographs were taken using a Nikon SMZ-U dissecting scope fitted with a Jenoptik ProgRes C5 camera.
Smz u dissecting scope
Manufactured by Jenoptik
The SMZ-U dissecting scope is a stereo microscope designed for detailed observation and examination of specimens. It features binocular viewing and a range of magnification settings to accommodate various sample sizes and requirements. The SMZ-U provides a clear, high-resolution image to support precise analysis and detailed work.
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Lab products found in correlation
3 protocols using smz u dissecting scope
1
Immunodetection of Neurofilaments in E10.5 Embryos
2
Neurofilament Immunodetection in E10.5 Embryos
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E10.5 embryos were fixed in 4% PFA in PBS at 4°C, rinsed in PBS, then permeabilized in PBS with 0.5% Triton X-100 for 24hrs at 4°C. Neurofilament immunodetection was performed as previously described (Ray et al., 2020 (link)). Primary anti-neurofilament antibody (DHSB, 2H3) was used at a 1:20 dilution and anti-mouse HRP-conjugated secondary antibody (Jackson ImmunoResearch, 115-035-003) was used at 1:1000 dilution. Signal was developed using the ImmPACT DAB Substrate Kit (Vector Laboratories, SK-4105). Photographs were taken using a Nikon SMZ-U dissecting scope fitted with a Jenoptik ProgRes C5 camera.
3
Skeletal Staining and Clearing
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E14.5, E16.5, E17.5, E18.5, and P0 embryos were skinned, eviscerated, and fixed in 95% ethanol overnight. Staining was performed with Alcian blue/Alizarin red (0.015% Alcian blue, 0.005% Alizarin red, 5% glacial acetic acid, 70% ethanol) overnight at 37°C. Skeletons were subsequently cleared in 1% KOH for 24hrs then transferred through a glycerol:KOH series of increasing glycerol concentration and decreasing KOH concentration. Skeletons were photographed in 80% glycerol using a Nikon SMZ-U dissecting scope fitted with a Jenoptik ProgRes C5 camera. Images of full P0 skeletons were merged using the Adobe Photoshop Photomerge function.
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