Pleconaril

Manufactured by Merck Group

Sourced in United States

Pleconaril is a laboratory instrument designed for the detection and analysis of specific biological targets. It utilizes advanced spectroscopic techniques to provide accurate and reliable data. The core function of Pleconaril is to facilitate research and investigation in various scientific domains.

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Lab products found in correlation

Rupintrivir, by Merck Group (4 mentions) Trypsin ethylenediaminetetraacetic acid, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) R523062, by Merck Group (1 mentions) R856932, by Merck Group (1 mentions) Screen well fda approved drug library v2 version 1, by Enzo Life Sciences (1 mentions) Hydroxy propyl β cyclodextrin hp β cd, by Tokyo Chemical Industry (1 mentions) Cholesterol, by Merck Group (1 mentions) Ribavirin, by Merck Group (1 mentions) Lamivudine, by Merck Group (1 mentions) Doxorubicin, by Merck Group (1 mentions) Cisplatin, by Teva Pharmaceuticals (1 mentions) Amantadine, by Merck Group (1 mentions) Etoposide, by Teva Pharmaceuticals (1 mentions)

5 protocols using pleconaril

Antiviral Screening of FDA-Approved Drugs

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Echovirus 30 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA) and propagated through the infection of RD cells, a human rhabdomyosarcoma cell line. RD cells were maintained in minimal essential medium (MEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution. MEM, FBS, trypsin-ethylenediaminetetraacetic acid, and antibiotic-antimycotic solution were purchased from Gibco BRL (Invitrogen Life Technologies, Karlsruhe, Germany). The antiviral activity of itraconazole against echovirus 30 was identified by in vitro screening of the Screen-Well^TM FDA Approved Drug Library V2 version 1.0 (BML-2843-0100; Enzo Life Sciences Inc., Farmingdale, NY, USA). Rupintrivir and pleconaril were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Lee J.S., Choi H.J., Song J.H., Ko H.J., Yoon K, & Seong J.M. (2017). Antiviral Activity of Itraconazole against Echovirus 30 Infection In Vitro. Osong Public Health and Research Perspectives, 8(5), 318-324.

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Compound storage and handling

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The capsid inhibitor pleconaril and picornavirus 3 C protease inhibitor rupintrivir were purchased from Sigma-Aldrich (St. Louis, MO). Compounds were stored in 500 µL aliquots at −80 °C, as 2 mM solutions, in high-performance liquid chromatography grade dimethyl sulfoxide (Sigma-Aldrich).

Zhang M., Zhang Y., Wang Y., Lv W, & Zhang Y. (2019). Automated cell-based luminescence assay for profiling antiviral compound activity against enteroviruses. Scientific Reports, 9, 6023.

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Enterovirus Infection Assay with Antivirals

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R523062 and R856932 were purchased from Sigma-Aldrich with Cat # of R523062 and R856932, respectively. Pleconaril was purchased from Sigma-Aldrich (Cat # SML0307). Telaprevir was purchased from Achemblock (Cat # F-4593). Rupintrivir was purchased from Sigma-Aldrich (Cat # PZ0315). Human rhabdomyosarcoma (RD; ATCC CCL-136) was maintained at 37 °C in a 5% CO₂ atmosphere and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin−streptomycin antibiotics. The following reagents were obtained through BEI Resources, NIAID, NIH: Human Enterovirus D68, US/MO/14–18949, NR-49130; Enterovirus D68, US/MO/14– 18947, NR-49129; Enterovirus D68, US/IL/14–18952, NR49131; Enterovirus D68, US/KY/14–18953, NR-49132; Enterovirus D68, US/IL/14–18956, NR-49133; Human Enterovirus A71 (EV-71), Tainan/4643/1998, NR-471; Human Enterovirus A71 (EV-71), MP4, NR-472. Enteroviruses A71 (EV-71) US/CT/2016–19519 and US/AK/2016–19516 strains were obtained from Dr. William Nix at the Centers for Disease Control and Prevention under a material transfer agreement. All viruses were amplified in RD cells prior to infection assays.

Ma C., Hu Y., Zhang J, & Wang J. (2020). Pharmacological characterization of the mechanism of action of R523062, a promising antiviral for enterovirus D68. ACS infectious diseases, 6(8), 2260-2270.

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Evaluation of ICZ's Antiviral Activity Against HRVs

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The antiviral activity of ICZ against HRVs was detected by in vitro screening of the Screen-Well FDA-Approved Drug Library V2 Version 1.0 (BML-2843-0100; Enzo Life Sciences, Lausanne, Switzerland). ICZ and hydroxypropyl-β-cyclodextrin (HP-β-CD) were purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). Cholesterol, Rupintrivir, and Pleconaril was obtained from Sigma-Aldrich (St. Louis, MO, USA). ICZ was dissolved in dimethyl sulfoxide at a concentration of 1 mM and diluted with cell culture medium for in vitro experiments. HP-β-CD was dissolved in distilled water at a concentration of 100 mg/ml (10%, w/v). The Cholesterol/CD complex solution was prepared by dissolving Cholesterol (2.5–20 mg/ml) in methanol by heating and diluting 100 times with HP-β-CD (10%, w/v) solution in distilled water. CD and Cholesterol/CD solutions were diluted with cell culture medium to appropriate concentrations for in vitro experiments.

Shim A., Song J.H., Kwon B.E., Lee J.J., Ahn J.H., Kim Y.J., Rhee K.J., Chang S.Y., Cha Y., Lee Y.S., Kweon M.N., Park K.S., Kim D.E., Cho S., Cho H.J, & Ko H.J. (2016). Therapeutic and prophylactic activity of itraconazole against human rhinovirus infection in a murine model. Scientific Reports, 6, 23110.

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Synergistic Antiviral-Cytotoxic Drug Interactions

Cited 1 time

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Amantadine, ribavirin, pleconaril, lamivudine, and doxorubicin were purchased from Sigma-Aldrich, acyclovir and ganciclovir from HEXAL AG, Holzkirchen, Germany. Retrovir was obtained from ViiV Healthcare, London, UK, Foscavir from Clinigen Healthcare, Staffordshire, UK and brivudine from Berlin Chemie, Germany. Etoposide and cisplatin were purchased from TEVA GmbH and 5FU from Medac, both Hamburg, Germany.
The simultaneous effect of antiviral drugs and cytostatics was analyzed by the isobologram method (50 % isodose) as described previously [30 (link)]. Briefly, the IC₅₀ for both substances were first determined using the MTT proliferation assay. Applying fixed percentages of the IC₅₀ for the first drug (20, 40, 60, 80 and 100 %) and varying the concentration of the second drug from 0.1 to 50 μM, the variation in the resulting IC₅₀ was determined for every percentage. The same procedure was carried out inversely for the second drug. Dose-response curves were then plotted and evaluated.

Díaz-Carballo D., Acikelli A.H., Klein J., Jastrow H., Dammann P., Wyganowski T., Guemues C., Gustmann S., Bardenheuer W., Malak S., Tefett N.S., Khosrawipour V., Giger-Pabst U., Tannapfel A, & Strumberg D. (2015). Therapeutic potential of antiviral drugs targeting chemorefractory colorectal adenocarcinoma cells overexpressing endogenous retroviral elements. Journal of Experimental & Clinical Cancer Research : CR, 34(1), 81.

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