A total of 2 ~ 3 mL of peripheral blood was collected in vials containing ethylenediamine tetraacetate acid from every participant, and DNA was extracted using a Blood Genomic DNA Extraction Kit (Xiamen Kaiso Biotech Co., Ltd., RC1001). The extracted DNA was assessed for purity (absorbance ratio of 260/280 nm between 1.8 and 2.0), and concentration using a UV spectrophotometer. After genetic counseling, pregnant women who were SMA carriers underwent interventional prenatal diagnosis and 10 mL amniotic fluid that was extracted at 18–24 weeks gestation was taken for detection. Genomic DNA was extracted using a Tissue Genome DNA Extraction Kit (Tiangen Biochemical Technology Co., Ltd., DP304, Peking), and the final DNA concentration was adjusted to between 20 and 40 ng/μL.
Tissue genome dna extraction kit
Manufactured by Tiangen Biotech
Sourced in China
The Tissue Genome DNA Extraction Kit is a laboratory equipment designed to extract and purify genomic DNA from various tissue samples. It provides a standardized and efficient method for isolating high-quality DNA for downstream applications, such as PCR, sequencing, and genetic analysis.
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Lab products found in correlation
2 protocols using tissue genome dna extraction kit
1
Prenatal Genetic Screening for SMA
2
Detecting Mycoplasma gallisepticum in Chickens
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To confirm whether the pathogen causing infection in chickens was MG, DNA from lung tissue was extracted using Tissue genome DNA extraction kit (No. DP304-02, TIANGEN Biotech Co., Ltd., Beijing, China) to detect MG-specific genes. The MGC gene of MG was amplified using PCR. Primer sequences (Sangon Biotech Corp., Shanghai, China) were as follows: mgc-F: 5′CTAGCAATCAAGCTGCAGGTACT-3′ mgc-R: 5′TGGTCCGTGTTGTGGATGAC-3′
The total reaction volume was 25 μL, including 12.5 μL of 2 × Taq PCR Master Mix, 1 μL of each primer, 2 μL cDNA, and 8.5 μL ddH2O. The reaction conditions were as follows: 95°C for 5 min; followed by 35 cycles of 95°C for 10 s, 56°C for 30 s, and 72°C for 60 s; and a final extension at 72°C for 5 min.
The total reaction volume was 25 μL, including 12.5 μL of 2 × Taq PCR Master Mix, 1 μL of each primer, 2 μL cDNA, and 8.5 μL ddH2O. The reaction conditions were as follows: 95°C for 5 min; followed by 35 cycles of 95°C for 10 s, 56°C for 30 s, and 72°C for 60 s; and a final extension at 72°C for 5 min.
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