Mouse caspase 1 p10

Manufactured by Santa Cruz Biotechnology

Mouse Caspase-1 p10 is a laboratory product for research purposes. It is a component of the Caspase-1 enzyme, which plays a role in the inflammatory response and programmed cell death. The product is intended for use in scientific investigations, but its specific applications are not provided in this factual description.

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Lab products found in correlation

Mouse il 1β, by R&D Systems (2 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions) Apr 004, by Alomone (1 mentions) A3854, by Merck Group (1 mentions) Sc 514, by Santa Cruz Biotechnology (1 mentions) Af 401 na, by Santa Cruz Biotechnology (1 mentions) Total jnk, by Cell Signaling Technology (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions)

2 protocols using mouse caspase 1 p10

Western Blot Analysis of Neutrophil Proteins

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Neutrophil lysates (20–30 μg protein) were fractionated in 12% SDS–PAGE, transferred onto nitrocellulose membranes, and incubated with primary antibodies—mouse IL-1β (R&D Systems, AF-401-NA at 1:1,300), mouse Caspase-1 p10 (Santa Cruz, SC-514 at 1:200), or with antibodies targeted to the intracellular C terminus or extracellular region of P2X₇R (Alomone Labs, catalog# APR 004 for the C terminal, and APR 008 for the ecto-domain, both at 1:200 dilution). These antibodies recognize mouse and human receptors. Loading controls were shown using antibodies to β actin (Sigma Aldrich A3854, 1:50 000). Reactivity was determined using HRP-conjugated secondary antibodies (Santa Cruz) and developed using Supersignal West Femto Maximum Sensitivity Substrate (Pierce). Images were cropped for presentation; full size images are presented in Supplementary Fig. 5.

Karmakar M., Katsnelson M.A., Dubyak G.R, & Pearlman E. (2016). Neutrophil P2X7 receptors mediate NLRP3 inflammasome-dependent IL-1β secretion in response to ATP. Nature Communications, 7, 10555.

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Corneal Protein Extraction and Analysis

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Corneas were excised from the eyes using a microscissor. Any associated iris was removed and clean corneas were homogenized in 1x cell lysis buffer (Cell Signaling Technology) supplemented with protease inhibitor cocktail. For in vitro experiment, 4x10⁶ cells were lysed in cell lysis buffer after appropriate stimulation. 20–30μg of protein were fractionated in 12% SDS-PAGE, transferred into nitrocellulose membrane and incubated with primary antibodies – mouse IL-1β (R&D Systems), mouse NLRP3 and ASC (Adipogen), human NLRP3 (Sigma Aldrich) and ASC (Adipogen), mouse Caspase-1p10 (Santa Cruz), mouse pJNK (T183/Y185) and total JNK (Cell Signaling Technology) and β actin (Sigma Aldrich). Reactivity was determined using HRP-conjugated secondary antibodies (Santa Cruz) and developed with Supersignal West Femto Maximum Sensitivity Substrate (Pierce).

Karmakar M., Katsnelson M., Malak H.A., Greene N.G., Howell S.J., Hise A.G., Camilli A., Kadioglu A., Dubyak G.R, & Pearlman E. (2015). Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation, and is dependent on K+ efflux. Journal of immunology (Baltimore, Md. : 1950), 194(4), 1763-1775.

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