The plumbous acetate [Pb(Ac)2] for Pb2+ supply was purchased from Sigma–Aldrich. Dulbecco's Modified Essential Medium (DMEM) and FBS were purchased from GIBCO Invitrogen. The Lyso-Tracker Red probes for acidic lysosome staining were from Beyotime. Anti-GRP78 (glucose-regulated protein 78), anti-GRP94, phosphorylates eukaryotic initiation factor 2 (anti-peIF2a), anti-cleaved caspase-3, anti-light chain 3 (anti-LC3) and anti-pS6 were purchased from Cell Signaling Technology. Anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase), BCL2-associated X protein (anti-Bax), B-cell lymphoma 2 (anti-BcL2) and anti-p70S6K (S6 kinase 1) antibodies were from Millipore. The p62 antibody was from Santa Cruz. Other reagents were of the highest purity available.
P62 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The P62 antibody is a laboratory reagent used in research applications. It specifically recognizes the p62 protein, also known as SQSTM1, which is involved in various cellular processes. The antibody can be utilized for techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and localization of the p62 protein.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified essential medium dmem, by Thermo Fisher Scientific (1 mentions)
Anti p70 s6k, by Merck Group (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti grp78, by Cell Signaling Technology (1 mentions)
Anti light chain 3 anti lc3, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Anti ps6, by Cell Signaling Technology (1 mentions)
Lc3 antibody, by Novus Biologicals (1 mentions)
Enhanced chemiluminescence ecl kit, by Bio-Rad (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions)
Mammalian cell lysis reagent, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Lc3 antibody, by Abcam (1 mentions)
Vectastain abc ap kit, by Vector Laboratories (1 mentions)
Hrp labeled goat anti rabbit secondary antibody, by Beyotime (1 mentions)
4 protocols using p62 antibody
1
Investigating Heavy Metal Toxicity in Cells
2
Macrophage Cell Line RAW 264.7 Characterization
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Mouse macrophage cell line RAW 264.7 was obtained from West China School of Pharmacy Sichuan University (Chengdu, China). RPMI-1640, phosphate buffered saline (PBS) and penicillin/streptomycin was purchased from Hyclone (USA). Fetal bovine serum (FBS) was purchased from Gibco (USA). Cell counting kit-8 (CCK-8) was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Mammalian Cell Lysis Reagent was purchased from Fermentas (USA). β-Actin antibody, P62 antibody, goat-anti-mouse-IgG-HRP and goat-anti-rabbit-IgG-HRP were purchased from Santa Cruz (USA). LC3 antibody was purchased from NOVUS (USA). Enhanced chemiluminescence (ECL) kit and PVDF membrane were purchased from Bio-Rad (USA). LPS (lipopolysaccharide) was purchased from Hycult Biotech (Netherlands).
3
Femur Tissue Immunohistochemical Analysis
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The paraffin-embedded femur tissues were sectioned at 3-μm thickness. After incubation with cleaved caspase-3 antibody (Cell Signalling Technology, Boston, MA, 1:100), LC3 antibody (Abcam, Cambridge, MA, 1: 200) and P62 antibody (Santa Cruz Biotechnology, CA, 1: 100) at 4 °C overnight, then incubated in HRP-labeled goat anti-rabbit secondary antibody (Beyotime Institute of Biotechnology, Inc., Jiangsu, China) for 30 min at 37 °C. After another 30-min incubation with Vectastain ABC-AP kit (Vector, CA), the specimens were rinsed and exposed to 3,3-diamino-benzidine (DAB) substrate for 3–10 min.
4
Evaluating Protein Expression in Rat Ventricle
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Western blots were performed as described previously9 (link). Briefly, the rats' left ventricle tissue and NRC samples were lysed in a lysis buffer containing 50 mM Tris, 150 mM NaCl, 1% Nonidet P-40, 0.25% superoxide dismutase, 1 mM EDTA, 1 mM NaF, 1 mM Na3VO3, 1 mM phenylmethylsulphonyl fluoride, and a proteinase inhibitor cocktail (Roche). The samples were evaluated by SDS-PAGE. Briefly, total protein from the samples was determined before being subjected to polyacrylamide gel electrophoresis and being transferred to a nitrocellulose (NC) membrane. The NC membrane was immunoblotted with an anti-14-3-3 antibody (Santa Cruz; 1:1,000), LC3 antibody (Santa Cruz; 1:1,000), p62 antibody (Santa Cruz; 1:1,000), and anti-β-actin antibody (Santa Cruz; 1:10,000). β-Actin protein served as a loading control. Protein bands were evaluated by densitometry using the Odyssey infrared imaging system (LI-COR).
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