The hydrocolloid of nano-Ag (AgNPs) obtained from Nano-Tech (Warsaw, Poland) was produced by an electric non-explosive patented method (patent number US2009020364 A1) from high-purity metals (99.9999%) and high-purity demineralized water [7 ]. The physical and chemical properties of AgNPs were characterized by Chwalibog et al. [8 (link)]. The shape and size of NPs were inspected with a Jeol JEM-1220 transmission electron microscope (TEM) at 80 KeV (JEOL, Tokyo, Japan), with a Morada 11 megapixel camera (Olympus Soft Imaging Solutions GmbH, Münster, Germany) (Figure 1 ). Samples of Ag for TEM were prepared by placing droplets of hydrocolloids onto formvar-coated copper grids (Agar Scientific Ltd, Stansted, UK). Nanoparticles of Ag were mostly spherical and polydispersed. The stability of the colloidal dispersions of the nanoparticles (zeta potential) was measured by the electrophoretic light-scattering method with a Zetasizer Nano ZS, model ZEN3500 (Malvern Instruments, Worcestershire, UK). The zeta potential of Ag nanoparticles was −36.4 mV, and the average diameter of particles was 70 nm (Figure 1 ). AgNPs were dissolved in ultra-pure water (Milli-Q water system, Millipore Corp., Billerica, MA, USA).![]()
Zetasizer nano zs model zen3500
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The ZetaSizer Nano ZS model ZEN3500 is a dynamic light scattering (DLS) instrument used for the measurement of particle size and zeta potential of samples in liquid dispersion. It is capable of measuring particle sizes from 0.3 nanometers to 10 micrometers and zeta potential from -500 to +500 millivolts.
Automatically generated - may contain errors
Lab products found in correlation
Jem 1220, by JEOL (2 mentions)
Jem 1220 tem, by JEOL (2 mentions)
Formvar coated copper grids, by Agar Scientific (1 mentions)
Morada 11 megapixel camera, by Olympus (1 mentions)
Milli q water system, by Merck Group (1 mentions)
System 2000, by PerkinElmer (1 mentions)
Ultrasonic bath, by Bandelin (1 mentions)
Atomic force microscopy, by Bruker (1 mentions)
Vertex 80v, by Bruker (1 mentions)
Jem 1220 transmission electron microscope, by JEOL (1 mentions)
Silver nitrate agno3, by Merck Group (1 mentions)
5 protocols using zetasizer nano zs model zen3500
1
Characterization of Silver Nanoparticles
2
Characterization of Graphene Oxide Aqueous Solution
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A GO water solution (4mg/mL) was obtained from Advanced Graphene Products (Zielona Gora, Poland). In the experiment, dilutions were prepared in deionized water and sonicated for 15 minutes before use.
The MIR spectrum of the GO water solution was registered with KRS round plates in transmission mode. Five separate spectra of the working sample were registered, and an averaged spectrum was calculated. The Perkin Elmer System 2000 spectrometer was used for spectra registration, and analysis was performed in the Pegrams software.
The morphology of the GO flakes was examined using a TEM - JEM-1220 (JEOL, Japan) at 80 kV and a TEM CCD Morada 11 megapixels camera (Olympus Soft Imaging Solutions, Germany). The zeta potential of the GO solution was measured using a ZetaSizer Nano ZS model ZEN3500 (Malvern Instruments, UK).
The MIR spectrum of the GO water solution was registered with KRS round plates in transmission mode. Five separate spectra of the working sample were registered, and an averaged spectrum was calculated. The Perkin Elmer System 2000 spectrometer was used for spectra registration, and analysis was performed in the Pegrams software.
The morphology of the GO flakes was examined using a TEM - JEM-1220 (JEOL, Japan) at 80 kV and a TEM CCD Morada 11 megapixels camera (Olympus Soft Imaging Solutions, Germany). The zeta potential of the GO solution was measured using a ZetaSizer Nano ZS model ZEN3500 (Malvern Instruments, UK).
3
Characterization of Graphene Oxide
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Single layer of graphene oxide, as water dispersion 2 wt.%, was purchased from US Research Nanomaterials, Inc. (Houston, TX, USA). GO flakes had thickness of 0.43–1.23 nm and diameter of 1.5–5.5 μm (manufacturer’s data).
The morphology of GO was characterized using a TEM-JEM-1220 (JEOL, Tokyo, Japan) at 80 kV and a TEM CCD Morada 11-megapixel camera (Olympus Soft Imaging Solutions, Munster, Germany). The zeta potential of GO solution was measured by light scattering using a ZetaSizer Nano ZS model ZEN3500 (Malvern Instruments, Malvern, UK). All measurements were performed in four repetitions. For the experiment, GO was diluted in ultrapure Milli-Q water at the final concentration of 100 ppm and sonicated in an ultrasonic bath (Bandelin Electronic, Berlin, Germany).
The morphology of GO was characterized using a TEM-JEM-1220 (JEOL, Tokyo, Japan) at 80 kV and a TEM CCD Morada 11-megapixel camera (Olympus Soft Imaging Solutions, Munster, Germany). The zeta potential of GO solution was measured by light scattering using a ZetaSizer Nano ZS model ZEN3500 (Malvern Instruments, Malvern, UK). All measurements were performed in four repetitions. For the experiment, GO was diluted in ultrapure Milli-Q water at the final concentration of 100 ppm and sonicated in an ultrasonic bath (Bandelin Electronic, Berlin, Germany).
4
Comprehensive Graphene Characterization
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Elemental analysis and Raman spectroscopy were conducted at the Institute of Electronic Materials Technology (Warsaw, Poland). Elemental analysis of graphene flakes was performed using an O836 oxygen analyzer (LECO, Katowice, Poland) to assess the content of oxygen in the samples. Electrical conductivity of the samples was also examined. The samples were first pressed to form thin pellets (<100 µm). Resistance was determined in Ohm/square (Ω/□) using a Hallotron (ECOPIA HMS 5500) with an external magnetic field of 0.55 T. Raman spectra were collected using a Reinshaw Invia confocal microscope with a 532-nm Nd-YAG laser, under a 100× objective with a 300-nm spot size and 1 mW laser. Measurements of the thickness of GO flakes were conducted with the use of Atomic Force Microscopy (Bruker, Stuttgart, Germany,). Chemical characterization of graphene samples was performed with an FTIR Spectrophotometer (Vertex 80v, Bruker). Zeta potential and size of flakes in water were measured by light scattering using a ZetaSizer Nano ZS model ZEN3500 (Malvern Instruments, Malvern, UK). The shape and size of GN and rGO flakes were examined using a JEM-1220 (JEOL, Tokyo, Japan) transmission electron microscope (TEM) at 80 KeV, and a Morada eleven-megapixel camera (Olympus Soft Imaging Solutions, Münster, Germany). Grids were inserted into the TEM immediately after drying the droplets in dry air.
5
Synthesis and Characterization of Nanoparticles
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All nanoparticles (NPs) used in this study were kindly provided by the Department of Animal Nutrition and Food Science, Warsaw University of Life Sciences, Poland. The pure silver nanoparticles (AgNPs) and pure gold nanoparticles (AuNPs) were obtained from Nano-Tech (Warsaw, Poland). Both hydrocolloids of AuNPs and AgNPs were produced by an electric non-explosive method from high purity metals and demineralized water (Polish patent 380649). The structures of the nanoparticles were visualized by a JEM-1220 transmission electron microscope (JEOL, Tokyo, Japan). Tannic acidmodified silver nanoparticles (AgTANPs) were synthesized by chemical reduction method using silver nitrate (AgNO 3 ) purity 99.999% (Sigma-Aldrich, St. Louis, MO, USA). AgTANPs were prepared by mixing the heated aqueous solution of AgNO 3 (95.2 g, 0.017%) with the aqueous solution of tannic acid (0.6 g, 5% C 76 H 52 O 46 Sigma-Aldrich). The long-term stability of the colloidal dispersions of all tested NPs (zeta potential) was measured and confirmed by the electrophoretic lightscattering method with a Zetasizer Nano ZS, model ZEN3500 (Malvern Instruments, Worcestershire, UK) (Orlowski et al. 2014; (link)Urbańska et al. 2015; (link)Zielinska et al. 2011 (link)).
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