To 1 mg/mL solutions of HSA, 0 mM, 0.01 mM, 0.1 mM and 1 mM of 4,4′-MDI were added and incubated with rotation at 37 °C for 2 h. Following incubation, each reaction was quenched with the addition of 0.5% acetic acid and vortex-mixed. Excess 4,4′-MDI was removed by centrifugation (10 min at 15,000 × g). Samples were transferred to autosampler vials for intact protein analysis via the Agilent 6530 UPLC/QTOF (Agilent, Santa Clara, CA) system as described below.
Agilent 6530 uplc qtof
Manufactured by Agilent Technologies
The Agilent 6530 UPLC/QTOF is a high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry system. It is designed for accurate mass analysis and identification of compounds.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using agilent 6530 uplc qtof
1
HSA Modification by 4,4'-MDI Quantification
2
HSA Protein Modification by 4,4′-MDI
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