Cells with various treatments were washed with PBS and fixed with 4% paraformaldehyde for 15 min at room temperature. After that, the cells were washed and treated with 0.4% Triton X-100. After 90 min, the cells were incubated with goat serum at room temperature for 60 min. Then the samples were incubated with anti-sarcomeric actin antibody (Sigma-Aldrich, St. Louis, MO, USA) at 4°C overnight. Next day, cells were stained with FITC-conjugate goat anti-mouse antibody for 1 h. Images were obtained under a fluorescence microscope (Nikon 80i, Japan). Cell surface area was determined using Image-Pro Plus Data Analysis Software.
Anti sarcomeric actin antibody
Manufactured by Merck Group
Sourced in United States
The Anti-sarcomeric actin antibody is a laboratory reagent used to detect and identify sarcomeric actin, a key structural protein found in muscle cells. It can be utilized in various immunological techniques, such as immunohistochemistry and Western blotting, to visualize and analyze the distribution and expression of sarcomeric actin in biological samples.
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Lab products found in correlation
2 protocols using anti sarcomeric actin antibody
1
Immunofluorescent Staining of Actin
2
Quantifying Myocardial Apoptosis by TUNEL
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Myocardial apoptosis was detecting by terminal deoxynucleotidyl transferase dUTP nick‐end labelling (TUNEL) staining using in situ Cell Death Detection kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's instructions. The sections were co‐stained with anti‐sarcomeric actin antibody (Sigma‐Aldrich) to specifically mark the cardiomyocytes and then stained with the secondary antibody (tetramethylrhodamine goat anti‐mouse antibody). Total cell nuclei were stained with 4,6‐diamidino‐2‐phenylindole (DAPI). More than 5 fields in >3 different sections/animals were examined by a technician who was not informed about treatment groups, in a blinded fashion.
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