Purified T cells were stimulated with plate bound anti-CD3 (2 µg/mL) and soluble anti-CD28 (2 µg/mL) antibodies for 24 hours. CD15+ Neutrophils were co-cultured with CD3+ T cells at a 5∶1 ratio in media supplemented with catalase (1000 U/mL) and superoxide dismutase (200 U/mL; Sigma) to neutralize reactive oxygen species. PD-L1 dependent T cell suppression was neutralized by addition of anti-PD-L1 antibody (clone 29E.2A3, Biolegend); isotype control antibody served as a control treatment (IgG2b; MCP-11; Biolegend). IFNγ ELISA was performed in cell culture media following manufacturer's protocol (eBioscience, San Diego, CA).
Anti pd l1 antibody clone 29e 2a3
Manufactured by BioLegend
Sourced in United States
The Anti-PD-L1 antibody (clone 29E.2A3) is a laboratory reagent used for research purposes. It is a monoclonal antibody that specifically binds to the PD-L1 (Programmed Death-Ligand 1) protein. PD-L1 is a key immune checkpoint molecule that plays a role in regulating immune responses.
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Lab products found in correlation
2 protocols using anti pd l1 antibody clone 29e 2a3
1
Neutralizing T cell suppression by PD-L1
2
Macrophage PD-L1 Expression Assay
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Macrophages were cultured in a 96-well microplate and stimulated with the CM of the adenocarcinoma cell lines for 1 day. After fixation with 1% paraformaldehyde, cells were reacted with anti-PD-L1 antibody (clone 29E.2A3; BioLegend, San Diego, CA, USA) or isotype-matched control antibody (BioLegend). After the cells were washed with phosphate-buffered saline, HRP-labeled anti-mouse immunoglobulin antibody (Nichirei) was added. Then, the plate was washed with phosphate-buffered saline, and tetramethylbenzidine developing solution (BioLegend) was used to visualize the positive signals.
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