Omniplex d neural data acquisition system

Manufactured by Plexon

Sourced in United States

The OmniPlex D Neural Data Acquisition System is a hardware and software platform designed for the acquisition and analysis of neural data. It provides the capability to record and process neural signals from multiple channels simultaneously.

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Lab products found in correlation

Neuroexplorer v4, by Plexon (2 mentions) Offline sorter v4, by Plexon (2 mentions) Matlab 8, by MathWorks (1 mentions) Hst 16o25 gen2 18p 2gp g1, by Plexon (1 mentions) Matlab, by MathWorks (1 mentions) Cm dii, by Thermo Fisher Scientific (1 mentions) Offline sorter software, by Plexon (1 mentions)

Close spelling variants of this product

OmniPlex system (54 citations) OmniPlex (48 citations) OmniPlex Neural Data Acquisition System (15 citations) Omniplex data acquisition system (10 citations) OmniPlex D (10 citations) Plexon data acquisition system (8 citations) Omniplex acquisition system (4 citations) Omniplex D System (4 citations) OmniPlex D Neural Acquisition System (2 citations) Omniplex Neural Acquisition system (2 citations)

15 protocols using omniplex d neural data acquisition system

Multichannel EEG Recording of Chronic KA-Induced Epilepsy

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After behavioural assays, multichannel electroencephalogram and intracranial local field potential (LFP) recording were captured from the different groups (control group and epilepsy group)
in the chronic phase of the KA-induced epilepsy model. We implanted a microwire array into the hippocampus of each mouse and performed multichannel EEG recordings in vivo as
described in our previous studies [21 (link)]. Briefly, an intracranial electrophysiological recording microwire array (Plexon, Dallas, TX, USA) was implanted
into the CA1 region of the right hippocampus anterior-posterior: 2.0 mm, medial-lateral: +1.5 mm, and dorsal-ventral: 1.5 mm, and affixed to the skull by dental cement in anesthetized mice.
Animals were allowed to recover from surgery for 1 week prior to the recordings. EEG tracings of KA-induced epilepsy in mice were recorded using the OmniPlex^® D Neural Data
Acquisition System (Plexon). The signals were preamplified (1,000×), filtered (0.1–1,000 Hz), and digitized at 4 kHz. EEG was recorded for 8 h per day for 7 days, beginning at 8:00 AM, to
avoid the interference of circadian cycle [5 (link)]. We used an OmniPlex^® Dneural Data Acquisition System (Plexon) to record EEGs, and defined an
electrophysiological seizure as a seizure with a high frequency (>5 Hz) and high amplitude (>2 times the baseline) that lasted for more than 5 s [22 (link)].

Zhang H., Zhou Z., Qin J., Yang J., Huang H., Yang X., Luo Z., Zheng Y., Peng Y., Chen Y, & Xu Z. (2023). Transmembrane protein modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models. Experimental Animals, 73(2), 162-174.

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Epilepsy Monitoring through Hippocampal EEG

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EEG recordings were captured from the different groups (control group and epilepsy group) in the chronic phase of the KA-induced epilepsy model. We implanted a microwire array into the hippocampus of each mouse and performed multichannel EEG recordings in vivo. We used an OmniPlex® Dneural Data Acquisition System (Plexon, Dallas, TX, USA) to record EEGs, and defined an electrophysiological seizure as a seizure with a high frequency (>5 Hz) and high amplitude (>2 times the baseline) that lasted for more than 5 s.

Tang S., Luo Z., Qiu X., Zhang Y., Lu X., huang H., Xu Z, & Xu Z. (2017). Interactions between GHRH and GABAARs in the brains of patients with epilepsy and in animal models of epilepsy. Scientific Reports, 7, 18110.

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Spectral Analysis of Frontal EEG Signals

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Recordings began 1-5 min before the start of drug exposure and ended 5 min after RORR. Signals were continuously recorded with an Omniplex D Neural Data Acquisition System (Plexon Inc., Dallas, TX, USA). Analogue signals were amplified with a 1× gain 16-channel headstage (HST/16o25-GEN2-18P-2GP-G1; Plexon) and a Plexon MiniDigiAmp gain of 1000. Signals were digitised with a sample rate of 40 kHz with a Plexon MiniDigiAmp, digitally filtered (Bessel, four poles, 200 Hz cut-off), and downsampled to 1 kHz in the OmniPlex Server.
In the dexmedetomidine dataset, EEG quality from two recording sessions was too poor for meaningful analysis, thus only data from the sessions with stable EEG signals were used. Power spectra of frontal EEGs were generated using MATLAB 8.4 (MathWorks Inc., Natick, MA, USA) and the Chronux software package (Cold Spring Harbor Laboratory; http://chronux.org/). Spectrograms were computed from frontal EEG recordings as described. 22 Frequency bands were defined as follows: delta (1-4 Hz), theta (4-8 Hz), alpha (8-12 Hz), beta (12-30 Hz), and low gamma (30-50 Hz).

Vincent K.F., Mallari O.G., Dillon E.J., Stewart V.G., Cho A.J., Dong Y., Edlow A.G., Ichinose F., Xie Z, & Solt K. (2023). Oestrous cycle affects emergence from anaesthesia with dexmedetomidine, but not propofol, isoflurane, or sevoflurane, in female rats. British journal of anaesthesia, 131(1).

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In vivo LFP Recording of Epileptiform Discharges

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After observation of SRSs (continuous observation for more than 30 days), we subsequently used mice that developed stable baseline of SRSs for in vivo LFP recordings using an OmniPlex® D neural Data Acquisition System (Plexon, Dallas, TX). LFP activity was continuously recorded and digitized at 4 kHz, filtered (0.1–1000 Hz) and preamplified (1000×) for 2 h per day for 7 days. Epileptiform-like discharge events were defined electrographically as high frequency (>5 Hz) and high amplitude (>2 times baseline) with a minimal duration of 5 s^{16 (link),19 (link)}. The duration of epileptiform activity was measured from the onset of the initial rise to the point when the LFP activity returned to baseline with no after-discharge period^{44 (link)}. For each recording session, we analyzed the frequency (events/min) and duration of epileptiform-like discharges. NeuroExplorer® v4.0 (Plexon, Dallas, TX) was used for data analysis of epileptiform-like discharge events.

Yang Q., Zheng F., Hu Y., Yang Y., Li Y., Chen G., Wang W., He M., Zhou R., Ma Y., Xu D., Tian X., Gao X., Wang Q, & Wang X. (2018). ZDHHC8 critically regulates seizure susceptibility in epilepsy. Cell Death & Disease, 9(8), 795.

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Electrophysiological Signals during Locomotion

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The electrophysiological signals were recorded on day 29 of the experimental timeline. Extracellular signals, i.e., spikes (sample rate 20 kHz, band-pass filtering 300–8000 Hz) and LFP (sample rate 1 kHz, band-pass filtering 0.5–200 Hz) were obtained using the 16-channel OmniPlex D Neural Data Acquisition System (Plexon, Dallas, TX, USA) in rats in resting (awake but not paying attention to their environment) and locomotion (ladder-walking) state. Videos were used to monitor animal conditions and recorded synchronously. The basal noise of the wideband (0.5–8000 Hz) signals indicated animal state differences compared to under resting conditions. Spike signals were recorded only when the signal-to-noise ratio was >2. During locomotion, the signals recorded throughout the walking task were extracted for analysis. Signal analysis was performed using Offline Sorter V4 (Plexon), Neuroexplorer V5 (Nex Technologies, Nays, KS, USA), and MATLAB (Mathworks, Natick, MA, USA).

Zhang H., Xie J., Li Y., Liu H., Liu C., Kan D., Geng X, & Wei S. (2021). Levodopa affects spike and local field synchronisation in the pedunculopontine nucleus of a rat model of Parkinson’s disease. Aging (Albany NY), 13(5), 7314-7329.

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Electrophysiological Recordings from dLGN

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A 32-channel probe (A4X8-10 mm-50-200-177-A32; Neuronexus, USA) was used to obtain the signal from the dLGN. At the beginning of each experiment, probes were coated with a fluorescent dye (CM-DiI; Invitrogen, UK) and then inserted into the dLGN using a one-axis oil hydraulic micromanipulator (model: MO-10; Narishige International Ltd., Japan). Spiking activity was amplified (gain 50), filtered (high-pass filter: 0.05 Hz), digitised (digitising frequency: 40 kHz) and entered to the OmniPlex D Neural Data Acquisition System (Plexon, Inc., USA). All dLGN neurons were identified based on their 1) high amplitude, 2) wide spike length, and 3) transient or sustained shape of response to full-field white light stimuli (Fig. 1B).

Jeczmien-Lazur J.S., Orlowska-Feuer P., Kustron A, & Lewandowski M.H. (2021). Short Wavelengths Contribution to Light-induced Responses and Irradiance Coding in the Rat Dorsal Lateral Geniculate Nucleus - An In vivo Electrophysiological Approach. Neuroscience, 468.

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Neural Signals During Treadmill Walking

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Using the 16-channel OmniPlex D Neural Data Acquisition System (Plexon Inc., Dallas, TX), LFP signals from the Pf and DS were recorded simultaneously for at least 30 min during periods of rest and treadmill walking. When recording during walking, two marks were manually added to the signal traces to indicate the times when the rat started to walk and stopped. Only signal segments between these marks were analyzed. The LFP signals were sampled at 1000 Hz with a band-pass filter set at 0.5 Hz−200 Hz; raw signal data were stored for later off-line analysis.

Zhang H., Yang J., Wang X., Yao X., Han H., Gao Y., Chang H., Xiang T., Sun S., Wang Y., Wang X, & Wang M. (2018). Altered Local Field Potential Relationship Between the Parafascicular Thalamic Nucleus and Dorsal Striatum in Hemiparkinsonian Rats. Neuroscience Bulletin, 35(2), 315-324.

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Multielectrode Neuronal Recording Technique

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The stimulating electrode was a stainless-steel bipolar electrode (50 μm in diameter; A.M. Systems. Inc., Sequim, Washington D.C., USA). The 12-channel recording electrodes consisted of three independent tetrodes. Each tetrode was formed by four twisted polyester-insulated nickel-chrome alloy wires (13 μm in diameter; STABLOHM 675, California Fine Wire Co, USA) with an impedance of 0.5–1 MΩ. All electrodes were secured to a microdrive (Fig 1A), which was constructed as described by Lin et al. [24 (link)].
The signals were amplified (×500), filtered (0.5–6,000 Hz), and stored in a computer (16 bits AD converter, 40 kHz sampling rate) using an OmniPlex D Neural Data Acquisition System (Plexon Inc., Dallas, Texas, USA).

Li J.J., Li Y.H., Gong H.Q., Liang P.J., Zhang P.M, & Lu Q.C. (2016). The Spatiotemporal Dynamics of Phase Synchronization during Epileptogenesis in Amygdala-Kindling Mice. PLoS ONE, 11(4), e0153897.

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Analyzing Hippocampal Local Field Potentials

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Local field potentials (LFPs) analysis was performed as described previously (Refs 44 , 45 (link)). After anesthetised by chloral hydrate (350 mg/kg, i.p.), the rats were received one treatment of miR-124 mimics or inhibitor or scrambled controls by intrahippocampal injection (anterior/posterior, −3.3 mm; medial/lateral, ±1.8 mm; dorsal/ventral, −2.6 mm). Then a recording micro wire array (4 × 4 array of platinum–iridium alloy wire, each with 25 µm diameter, Plexon, Dallas, TX) was implanted into the right dorsal hippocampus (anterior/posterior, −3.7 mm; medial/lateral, −2.5 mm; dorsal/ventral, −2.7 mm). The rats which were treated surgery were recovered for 5 days before the electrophysiological activity was recorded. OmniPlex^® D neural Data Acquisition System (Plexon, Dallas, TX) was used recording for LFPs. The LFPs signals were digitised at 4 kHz, filtered (0.1–1000 Hz) and preamplified (1000×). Neuro Explorer v4.0 (Plexon, Dallas, TX) was used for the LFPs data analysis. Seizures were induced in rats by lithium chloride-pilocarpine and the electrophysiological was continuously recorded (more than 80 min). A typical seizure discharged of electrophysiological was showing as high-frequency (frequency >5 Hz), high-amplitude discharge (amplitude >2 times the baseline) and lasting longer than 5 s (Ref. 44 ).

Wang W., Wang X., Chen L., Zhang Y., Xu Z., Liu J., Jiang G., Li J., Zhang X., Wang K., Wang J., Chen G, & Luo J. (2016). The microRNA miR-124 suppresses seizure activity and regulates CREB1 activity. Expert Reviews in Molecular Medicine, 18, e4.

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Induction and Electrophysiological Analysis of Seizures in Rats

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After SAD-B-siRNA was injected into the CA1 region (AP –3.6 mm, ML –2.6 mm, and DV –2.8 mm), a microwire array (a 1.5×6 array of platinum-iridium alloy wires, each with a 20-μm diameter) was implanted into the same site in the CA1 region of the hippocampus. PTZ was administered to induce seizures (after two weeks). In vivo multichannel EEG recordings were continuously collected for 10 min after the administration of PTZ. According to the PTZ scoring criteria, an electrophysiological seizure was defined as the manifestation of seizure behaviors ranging from stages 1–4 in the rats. The intra-hippocampal LFP of electroencephalography (EEG) recordings showed a high-amplitude discharge (>3-fold higher the baseline) that began in the hippocampus and spread to the cortex with a high frequency (>5 Hz). Latencies and generalized tonic clonic seizures (GTCs) in the observed 10 min period during status epilepticus were recorded for each animal from each group. LFPs were preamplified (1000×), filtered (0.1–1000 Hz), and digitized at 4 kHz using an OmniPlex D Neural Data Acquisition System (Plexon, USA). All animals were euthanized with pentobarbital (100 mg/kg, ip, Sigma) after the seizures were recorded and hippocampal tissues were collected for morphological and biochemical studies.

Li R., He M., Wu B., Zhang P., Zhang Q, & Chen Y. (2020). SAD-B modulates epileptic seizure by regulating AMPA receptors in patients with temporal lobe epilepsy and in the PTZ-induced epileptic model. Brazilian Journal of Medical and Biological Research, 53(4), e9175.

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