D saccharic 1 4 lactone

Manufactured by Merck Group

Sourced in United States

D-saccharic-1,4-lactone is a chemical compound used as a laboratory reagent. It is a cyclic ester derived from D-glucaric acid. This product is suitable for use in various analytical and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Alamethicin, by Merck Group (7 mentions) Uridine diphosphate glucuronic acid, by Merck Group (5 mentions) Ko143, by Merck Group (3 mentions) Propofol, by Merck Group (2 mentions) Mk571, by Merck Group (2 mentions) Anti bcrp, by OriGene (2 mentions) Anti mrp1, by OriGene (2 mentions) Anti mrp2, by OriGene (2 mentions) Anti mrp3, by OriGene (2 mentions) Anti mrp4, by OriGene (2 mentions) Nicotinamide adenine dinucleotide phosphate, by Merck Group (1 mentions) β estradiol 3 o glucuronide, by LGC (1 mentions) β estradiol, by LGC (1 mentions) Magnesium chloride mgcl2, by Merck Group (1 mentions) Dipyridamole dipy, by Merck Group (1 mentions) Leukotriene c4 ltc4, by Merck Group (1 mentions) β estradiol, by Merck Group (1 mentions) Propofol o glucuronide, by LGC (1 mentions) Magnesium chloride, by Merck Group (1 mentions) Rat liver microsomes, by Corning (1 mentions)

Close spelling variants of this product

D-saccharic acid 1,4-lactone D-saccharic-1,4-lactone monohydrate D-saccharic acid 1,4-lactone monohydrate D-saccharic-1 4-lactone

7 protocols using d saccharic 1 4 lactone

Glucuronidation of Ginger Bioactives

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Alamethicin, d-saccharic-1,4-lactone, magnesium chloride, and uridine diphosphate glucuronic acid (UDPGA), were provided from Sigma-Aldrich (St Louis, MO). Pooled human liver microsomes (HLM), human intestine microsomes (HIM) and recombinant expressed human UGT Supersomes™ (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 1A10, 2B4, 2B7, 2B10, 2B15 and 2B17) were obtained from Corning Biosciences (New York, USA). Zidovudine (AZT) were purchased from Aladdin Chemicals (Shanghai, China). [6]-shogaol (6S), [8]-shogaol (8S), [10]-shogaol (10S) with purity over 99% were isolated and identified in our laboratory, and their 13C-NMR and HRMS data were listed in the ESI.† Other chemicals and materials were all analytical grade.

He L., Xu J., Wang Q., Zhang Y., Qin Z., Yu Y., Qian Z., Yao Z, & Yao X. (2018). Glucuronidation of [6]-shogaol, [8]-shogaol and [10]-shogaol by human tissues and expressed UGT enzymes: identification of UGT2B7 as the major contributor. RSC Advances, 8(72), 41368-41375.

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Comprehensive Evaluation of Xenobiotic Metabolism

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Nicotinamide adenine dinucleotide phosphate (NADPH), uridine diphosphate glucuronic acid (UDPGA), magnesium chloride (MgCl₂), alamethicin and D-saccharic-1, 4-lactone were all provided from Sigma-Aldrich (St Louis, MO). Pooled human liver microsomes (HLM), twelve individual pooled human liver micro-somes (iHLM), pooled human intestine microsomes (HIM), rat liver microsomes (RLM), mice liver microsomes (MLM), guinea pig liver microsomes (GpLM), monkey liver microsomes (MkLM), dog liver microsomes (DLM), rabbit liver microsomes (RaLM), expressed human CYPs (CYP1A1, 1A2, 1B1, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4 and 3A5) and human UGTs (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 2B4, 2B7, 2B10, 2B15 and 2B17) were all obtained from Corning Biosciences (New York, USA). Corylin (purity > 98%) were purchased from Chengdu Chroma-Biotechnology Co., Ltd. (Chengdu, China). β-estradiol, chenodeoxycholic acid (CDCA), phenacetin and tolbutamide were purchased from Aladdin Chemicals (Shanghai, China). All other chemicals and reagents were analytical grade commercially available.

Qin Z., Li S., Yao Z., Hong X., Xu J., Lin P., Zhao G., Gonzalez F.J, & Yao X. (2018). Metabolic profiling of corylin in vivo and in vitro. Journal of pharmaceutical and biomedical analysis, 155, 157-168.

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Demethoxycurcumin Glucuronidation by UGT1A1

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Demethoxycurcumin (DMC) was provided by Guangzhou Fans Biotechnology Co., Ltd. (Guangzhou, China). Alamethicin, D-saccharic-1,4-lactone, magnesium chloride (MgCl₂), MK571, Ko143, propofol, and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St. Louis, MO). Human UGT1A1 and individual human liver microsomes (iHLM, n = 12) were obtained from Corning Biosciences (New York, USA). UGT1A1-overexpressing HeLa cells (HeLa1A1 cells) were established as described in a previous study [22 (link)] and provided by Prof. Baojian Wu, who works in Jinan University in Guangzhou of China. β-Estradiol and β-Estradiol-3-O-glucuronide were purchased from Toronto Research Chemicals (North York, ON, Canada). The anti-UGT1A1 antibody was purchased from BD Biosciences (Woburn, MA). The anti-BCRP (catalog number TA322704), anti-MRP1 (catalog number TA309559), anti-MRP2 (catalog number TA313641), anti-MRP3 (catalog number TA314800), and anti-MRP4 (catalog number TA327332) antibodies were purchased from OriGene Technologies (Rockville, MD). All other chemicals and reagents (analytical grade or better) were commercially available.

Zhang B., Yang J., Qin Z., Li S., Xu J., Yao Z., Zhang X., Gonzalez F.J, & Yao X. (2019). Mechanism of the efflux transport of demethoxycurcumin-O-glucuronides in HeLa cells stably transfected with UDP-glucuronosyltransferase 1A1. PLoS ONE, 14(5), e0217695.

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Lentiviral Vector Construction and Characterization

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The pLVX-mCMV-ZsGreen-PGK-Puro vector [9371 base pairs (bp)], and a pLVX-shRNA2-Neo vector (9070 bp) were obtained from BioWit Technologies (Shenzhen, China). Expressed human UGT1A1 was purchased from Corning Biosciences (New York). Alamethicin, β-estradiol, β-estradiol-3-O-glucuronide, dipyridamole (DIPY), D-saccharic-1,4-lactone, magnesium chloride (MgCl₂), MK-571, Ko143, leukotriene C4 (LTC4), and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St Louis, MO). UGT1A1, GADPH, BCRP, MRP1, MRP3, and MRP4 antibodies were all purchased from OriGene Technologies (Rockville, MD). Bisdemethoxycurcumin was provided from Guangzhou Fans Biotechnology Co., Ltd (Guangzhou, China). All other chemicals and reagents were of guaranteed reagent or the highest grade commercially available.

Yang J., Zhang B., Qin Z., Li S., Xu J., Yao Z., Zhang X., Gonzalez F.J, & Yao X. (2018). Efflux excretion of bisdemethoxycurcumin-O-glucuronide in UGT1A1-overexpressing HeLa cells: Identification of breast cancer resistance protein (BCRP) and multidrug resistance-associated proteins 1 (MRP1) as the glucuronide transporters. BioFactors (Oxford, England), 44(6), 558-569.

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Establishment and Characterization of UGT1A9-Overexpressing HeLa Cells

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Human UGT1A9-overexpressing HeLa cells (HeLa1A9 cells) were established as described previously (Jiang et al., 2011 (link)), and were provided by Prof. Baojian Wu who works in Jinan University in Guangzhou of China. Alamethicin, D-saccharic-1,4-lactone, magnesium chloride (MgCl₂), MK571, Ko143, propofol, and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St Louis, MO, United States), whereas propofol-O-glucuronide from Toronto Research Chemicals (North York, ON, Canada). Fraxetin was provided by Guangzhou Fans Biotechnology Co., Ltd., (Guangzhou, China), and human UGT1A9 was purchased from Corning Biosciences (New York, NY, United States). UGT1A9 antibody was purchased from BD Biosciences (Woburn, MA, United States). The anti-BCRP, anti-MRP1, anti-MRP2, anti-MRP3, and anti-MRP4 antibodies were purchased from OriGene Technologies (Rockville, MD, United States). All other chemicals and reagents (analytical grade or better) were commercially available.

Qin Z., Zhang B., Yang J., Li S., Xu J., Yao Z., Zhang X., Gonzalez F.J, & Yao X. (2019). The Efflux Mechanism of Fraxetin-O-Glucuronides in UGT1A9-Transfected HeLa Cells: Identification of Multidrug Resistance-Associated Proteins 3 and 4 (MRP3/4) as the Important Contributors. Frontiers in Pharmacology, 10, 496.

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Wushanicaritin Glucuronidation in Various Species

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Uridine diphosphate glucuronic acid (UDPGA), magnesium chloride (MgCl₂), alamethicin and d-saccharic-1,4-lactone were provided by Sigma-Aldrich (St. Louis, MO, USA). Pooled human liver microsomes (HLM), human intestinal microsomes (HIM), recombinant expressed human UGT Supersomes™ (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 2B4, 2B7, 2B10, 2B15 and 2B17), dog liver microsomes (DLM), rat liver microsomes (RLM), monkey liver microsomes (MkLM), rabbit liver microsomes (RaLM) and guinea pig liver microsomes (GpLM) were all obtained from Corning Biosciences (Corning, Corning, NY, USA). Wushanicaritin (purity > 98%) was purchased from Jingzhu Medical Technology Co., Ltd. (Nanjing, China). Wushanicaritin 3-O-glucuronidation (G1) and 7-O-glucuronidation (G2) were prepared according to the previous study [12 (link)]. The detailed NMR data of G1 and G2 are shown in Table S1, and their NMR spectra were displayed in Figures S1 and S2. β-estradiol, chenodeoxycholic acid (CDCA), propofol and zidovudine (AZT) were purchased from Aladdin Chemicals (Shanghai, China). All other chemicals and reagents were of analytical grade or the highest grade commercially available.

Hong X., Zheng Y., Qin Z., Wu B., Dai Y., Gao H., Yao Z., Gonzalez F.J, & Yao X. (2017). In Vitro Glucuronidation of Wushanicaritin by Liver Microsomes, Intestine Microsomes and Expressed Human UDP-Glucuronosyltransferase Enzymes. International Journal of Molecular Sciences, 18(9), 1983.

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In Vitro Metabolism of Icaritin

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Uridine diphosphate glucuronic acid (UDPGA), magnesium chloride (MgCl₂), alamethicin and D-saccharic-1, 4-lactone were provided from Sigma-Aldrich (St Louis, MO). Pooled human liver microsomes (HLM), pooled human intestine microsomes (HIM) and recombinant expressed human UGT Supersomes™ (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 2B4, 2B7, 2B10, 2B15 and 2B17) were obtained from Corning Biosciences (New York, NY). Icaritin (purity>98%) were purchased from Jingzhu Medical Technology Co., Ltd (Nanjing, China). Chenodeoxycholic acid (CDCA), propofol and zidovudine (AZT) were purchased from Aladdin Chemicals (Shanghai, China). All other chemicals and reagents were analytical grade or the highest grade commercially available.

Wang L., Hong X., Yao Z., Dai Y., Zhao G., Qin Z., Wu B., Gonzalez F.J, & Yao X. (2017). Glucuronidation of icaritin by human liver microsomes, human intestine microsomes and expressed UDP-glucuronosyltransferase enzymes: identification of UGT1A3, 1A9 and 2B7 as the main contributing enzymes. Xenobiotica; the fate of foreign compounds in biological systems, 48(4), 357-367.

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