Sections were deparaffinized, rehydrated in graded ethanol, boiled in citrate buffer for antigen retrieval, and stained with a primary antibody for FABP4 (1:200, #ab13979, Abcam, Cambridge, UK) and CD146 (1:200, #ab75769, Abcam). HRP-conjugated secondary antibody (#87–8963, Invitrogen) was used (#00–1111, Invitrogen).
Ab13979
Manufactured by Abcam
Sourced in United Kingdom
Ab13979 is a mouse monoclonal antibody that recognizes Human CD4 protein. The antibody is suitable for use in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab75769, by Abcam (2 mentions)
Hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
A11008, by Thermo Fisher Scientific (1 mentions)
A21240, by Thermo Fisher Scientific (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
P erk thr202 tyr204, by Cell Signaling Technology (1 mentions)
Erk1 2, by Santa Cruz Biotechnology (1 mentions)
Prb 160p, by Fortrea (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bs1813r, by Bioss Antibodies (1 mentions)
Ab19845, by Abcam (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Imagequant las 4000 mini instrument, by GE Healthcare (1 mentions)
Ab9485, by Abcam (1 mentions)
Las 4000, by Cytiva (1 mentions)
4 protocols using ab13979
1
Immunohistochemical Analysis of FABP4 and CD146
2
Immunostaining of Malaria Gametocytes and Bone Cells
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Ten-μm-thick sections were air-dried, hydrated with PBS and exposed to 5% goat serum in PBS for 30 minutes at RT. For immunostaining of gametocytes, the sections were incubated with a mouse monoclonal anti-Pfs16 antibody (Bruce et al., 1994 (link)) (32F717-SF, kindly provided by Dr. R. Sauerwein, Radboud University Medical Centre, Nijmegen, The Netherlands), at a dilution of 1:500 in PBS, O/N at 4°C. For immunostaining of cells within the ossicles the following antisera were used: rabbit polyclonal anti-mouse and human Fatty Acid Binding Protein (FABP4, ab13979, Abcam Cambridge, UK), anti-human CD146 (ab75769, Abcam), anti-human Alkaline Phosphatase (ALP, 11187-1-AP, ProteinTech Manchester, UK) all applied at 1:100 in PBS, O/N at +4°C. Following washing with PBS, samples were incubated with Alexa Fluor 647-conjugated goat anti-mouse (A-21240, Thermo Fisher Scientific) or Alexa Fluor 488-conjugated goat anti-rabbit (A-11008, Thermo Fisher Scientific) at dilution of 1:200 in PBS for 1 hr at RT. Nuclei were counterstained with TO-PRO-3.
3
Immunohistochemical Analysis of Skin Samples
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The antibodies used in this study were as follows: K5 (1:1000, Covance #PRB-160P), keratin 10 (K10) for immunohistochemistry (1:100, Covance #MS-159), K10 for western blot (1:10000, Abcam #ab76318), K15 (1:100, Thermo Fisher Scientific #MS-1068; 1:50, Santa Cruz #sc-47697), loricrin (1:1000, Covance #PRB-145P), Ki67 (1:150, DAKO #M7249), p63 (1:50, Santa Cruz #sc-8431), E-cadherin (1:40, Cell Signaling #3195), ERK1/2 (1:50, Santa Cruz #sc-514302), p-ERK (Thr202/Tyr204, 1:200, Cell Signaling #4370), CD34 (1:50, eBioscience #14-0341-82) and FABP4 (1:200, Abcam #ab13979). For detecting WWOX expression, an anti-WWOX antibody recognizing both human and mouse proteins was used (Chang et al., 2001 (link), 2005b (link)).
4
Protein Extraction and Western Blot Analysis
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The cells were extracted into RIPA lysis buffer (Beyotime, Beijing, China) containing a protease inhibitor cocktail (Sigma-Aldrich, Saint Louis, MO, USA), and the lysates were centrifuged at 12,000 rpm (4°C for 15 min). Protein concentration was quantified using a BCA protein assay kit (Thermo Scientific, Waltham, MA, USA). The following primary antibodies were used: rabbit anti-FABP4 (1:500; ab13979; Abcam, Cambridge, MA, USA), rabbit anti-FABP5 (1:1,000; bs-2370R; Bioss, Bejing, China), rabbit anti-PPAR-γ (1:1,000; 2443S; Cell Signaling Technology, Danvers, MA, USA) and rabbit anti-CADM2 (1:1,000; bs-8246R; Bioss, Bejing, China), rabbit anti-CADM3 (1:200; 343894; USBiological, Swampscott, MA, USA), rabbit anti-CADM4 (1:1,000; bs-5997R; Bioss, Bejing, China), rabbit anti-HDAC1 (1:1,000; ab19845; Abcam, Cambridge, MA, USA), rabbit anti-HDAC2 (1:1,000; bs1813R; Bioss, Bejing, China), rabbit anti-HDAC6 (1:1,000; bs-2811R; Bioss, Bejing, China), rabbit anti-HDAC8 (1:1,000; bsm-52088R; Bioss, Bejing, China), rabbit anti-GAPDH (1:1,000; ab9485; Abcam, Cambridge, MA, USA) were used as a control. Signal detection was performed using an ImageQuant LAS 4000 mini instrument (GE Healthcare, Piscataway, NJ, USA).
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