Procyanidin B1, procyanidin B2 and procyanidin C1 were acquired from Chromadex (Irvine, California, USA). (+)-catechin, (−)-epicatechin, quercetin glucoside, phloridzin, chlorogenic acid, horseradish peroxidase type I, hydrogen peroxide (H2O2), ABTS (2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid), potassium persulfate, potassium chloride (KCl), cupric sulfate pentahydrate (CuSO4.5H2O), hexamine, murexide, phloroglucinol, sodium carbonate, ascorbic acid, acetic acid, formic acid, ethanol, acetone, and methanol were purchased from Sigma-Aldrich (St. Louis MO, USA). Acetonitrile and hydrochloric acid were acquired from J.T. Baker (Edo. de Méx., México). Deionized water (18 MΩ•cm−1) was prepared with a Milli-Q system (Millipore, Bedford, MA, USA).
Abts 2 2 azinobis 3 ethylbenzthiazoline 6 sulfonic acid
Manufactured by Merck Group
Sourced in United States
ABTS (2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid)) is a chemical compound used as a substrate in various assays and enzymatic reactions. It is a common oxidation-reduction indicator, frequently employed in colorimetric and spectrophotometric applications.
Automatically generated - may contain errors
Lab products found in correlation
Ascorbic acid, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Phloridzin, by Merck Group (1 mentions)
Murexide, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Procyanidin c1, by ChromaDex (1 mentions)
Procyanidin b1, by ChromaDex (1 mentions)
Horseradish peroxidase type 1, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Hydrogen peroxide h2o2, by Merck Group (1 mentions)
Epicatechin, by Merck Group (1 mentions)
Phloroglucinol, by Merck Group (1 mentions)
Hexamine, by Merck Group (1 mentions)
4 protocols using abts 2 2 azinobis 3 ethylbenzthiazoline 6 sulfonic acid
1
Polyphenol Quantification and Antioxidant Assays
2
Thyme Essential Oil Chitosan Nanocarriers
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Thyme essential oil (ρ = 0.917 g/cm3 at 25 °C) from Sigma-Aldrich® (St. Louis, MO, USA) (Lot: MKCF4333) is used to form the oil nucleus. Chitosan (MW = 50–190 kDa, μ = 20–300 cP in CH3COOH solution (1 g/L) at 25 °C) (Lot: STBH6262) as the encapsulating polymer, Tween® 80 and Span® 80 (Lot: MKBV4425V) were purchased from Sigma Aldrich®. The distilled water was Milli-Q® grade (Millipore Corporation, Bedford, MA, USA). ABTS (2,2′-azino-bis- (3-ethylbenzthiazoline)-6-sulfonic acid) (Lot: SLBD8908V), DPPH (2,2-diphenyl-1-picrylhydracil) (Lot: STBG9431), FRAP (2,4,6-tri (2-pyridyl)-s-triazine) (Lot: BCBW6989) were purchased from Sigma Aldrich® (St. Louis, MO, USA). Ascorbic acid (C6H8O6) (Lot: A0616289), potassium persulfate (K2S2O8) (Lot: M1010511), potassium dihydrogen phosphate (KH2PO4) (Lot: M0918575), dipotassium hydrogen phosphate (KH2PO4) (Lot: M1014575), and glacial acetic acid (CH3-COOH) (Lot: A0414235) were purchased from Meyer® (Reactivos Química Meyer, Ciudad de México, México). Cyclohexane (C6H12) and methanol (CH3OH) (Lot: 824333) were purchased from Fermont® (Productos Químicos Monterrey, México).
3
Potent Enzyme Activity Quantification
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The solvents
used were of analytical grade (El-Gomhouria Co., Cairo, Egypt). Renin
enzyme, renin substrate (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys
(Dabcyl)-Arg), ACE extracted from rabbit lung, ACE substrate (Hippuryl-histidyl-leucine,
HHL), Quinapril, ABTS (2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic
acid), and ascorbic acid were all obtained from Sigma-Aldrich Chemicals,
Germany. Sodium phosphate, ammonium molybdate, potassium persulfate,
potassium ferricyanide, and ferric chloride were purchased from El-Nasr
Company for Pharmaceutical Chemicals, Egypt. Phosphate buffer was
bought from Bio diagnostic, Egypt. HPLC-grade acetonitrile, trichloroacetic
acid, and methanol were purchased from SDFCL Fine-Chem Limited, Mumbai,
India. Round bottom and black microplates (96 wells) were obtained
from Corning Incorporated (USA).
used were of analytical grade (El-Gomhouria Co., Cairo, Egypt). Renin
enzyme, renin substrate (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys
(Dabcyl)-Arg), ACE extracted from rabbit lung, ACE substrate (Hippuryl-histidyl-leucine,
HHL), Quinapril, ABTS (2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic
acid), and ascorbic acid were all obtained from Sigma-Aldrich Chemicals,
Germany. Sodium phosphate, ammonium molybdate, potassium persulfate,
potassium ferricyanide, and ferric chloride were purchased from El-Nasr
Company for Pharmaceutical Chemicals, Egypt. Phosphate buffer was
bought from Bio diagnostic, Egypt. HPLC-grade acetonitrile, trichloroacetic
acid, and methanol were purchased from SDFCL Fine-Chem Limited, Mumbai,
India. Round bottom and black microplates (96 wells) were obtained
from Corning Incorporated (USA).
4
Elemental Analysis of Antioxidants
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Chemicals and reagents used in elemental analysis such as H2SO4, NaOH, HNO3, HClO4, and C2SO4 were purchased from Sigma-Aldrich (USA) while Acetic acid from Dae-Jung Korea. The antioxidant chemicals such as DPPH (2, 2-Diphenyl-1-picrylhydrazyl) and ABTS (2, 2-Azino bis [3-ethylbenzthiazoline]-6-sulfonic acid) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol was obtained from Merck: Germany. All chemicals were of analytical grade except HPLC solvents which were of HPLC grade.
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