Rabbit monoclonal anti aldh1a1 ab

Culture media (RPMI-1640, MEM, and DMEM), fetal bovine serum, antibiotic-antimycotic solution, L-glutamine, sodium private, DAPI mounting medium, and DOX and MTX were purchased from Sigma (St. Louis, MO, USA). Rabbit polyclonal anti-OSF-2 Ab was obtained from Proteintech (Manchester, UK), rabbit monoclonal anti-ALDH1A1 Ab was purchased from Abcam (Cambridge, UK), mouse monoclonal anti-ALDH1A1 Ab was purchased from ABGENT (San Diego, CA, USA), and rabbit polyclonal anti-GADPH Ab was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Goat policlonal anti-rabbit horseradish peroxidase (HRP)-conjugated Ab was purchased from Cell Signaling (Danvers, MA, USA). The fluorescent secondary antibodies—Alexa Fluor^®488 Donkey Anti-Rabbit and Alexa Fluor^®594 Donkey Anti-mouse IgG—were obtained from Jackson ImmunoResearch Laboratories (Cambridgeshire, UK). Western blot reagents (MEMbranes, gels, and protein markers) were purchased from Biorad (Bio-Rad Laboratories, Hemel Hempstead, UK).

Culture media (RPMI-1640), fetal bovine serum, antibiotic–antimycotic solution, L-glutamine, DAPI mounting medium, and PAC and TOP were purchased from Sigma (Sigma-Aldrich, Poznan, Poland). Rabbit monoclonal anti-ALDH1A1 Ab was purchased from Abcam (Abcam, Cambridge, UK), mouse monoclonal anti-COL3A1 was purchased from Invitrogen (Invitrogen, Carlsbad, CA, USA), mouse monoclonal anti-PTPRK Ab, anti-p-Tyr, mouse monoclonal anti-MYOT Ab (B-3), and rabbit polyclonal anti-GADPH Ab were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal anti-P-gp Ab was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Rabbit polyclonal anti-TGFBI was purchased from Atlas Antibodies (Stockholm, Sweden). Mouse anti-Ki67 was purchased from Dako (Glostrup, Denmark). Donkey anti-goat horseradish peroxidase (HRP)-conjugated Ab was purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology Inc., Dallas, TX, USA). The fluorescent MFP488 donkey anti-goat IgG was obtained from MoBiTec (MoBiTec, Molecular Biotechnology, Goettingen, Germany) and fluorescent Alexa Fluor^®488 and Alexa Fluor^®594 Donkey Anti-Rabbit IgG from Jackson ImmunoResearch Laboratories (Jackson ImmunoResearch Laboratories, Cambridgeshire, UK). Western blot reagents (membranes, gels and protein marker) were purchased from Bio-Rad (Bio-Rad Laboratories Ltd., Watford, Hertfirdshire, UK).

Culture media (RPMI-1640 and MEM), fetal bovine serum, antibiotic–antimycotic solution, l-glutamine, DAPI mounting medium and PAC and TOP were purchased from Sigma (Sigma-Aldrich, Poznań, Poland). Rabbit polyclonal anti-LOX Ab was obtained from Proteintech (Proteintech Europe, Manchester, UK), rabbit monoclonal anti-ALDH1A1 Ab was purchased from Abcam (Abcam, Cambridge, UK), mouse monoclonal anti-ALDH1A1, and goat polyclonal anti-COL1A2, -COL3A1, and -COL21A1 Abs were purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology Inc, Dallas, TX, USA). Mouse monoclonal anti-PGP Ab was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Donkey anti-goat horseradish peroxidase (HRP)-conjugated Ab was purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology Inc., Dallas, TX, USA). The fluorescent MFP488 donkey anti-goat IgG was obtained from MoBiTec (MoBiTec, Molecular Biotechnology, Goettingen, Germany, MFP488) and fluorescent Alexa Fluor^®488 and Alexa Fluor^®594 Donkey Anti-Rabbit IgG from Jackson ImmunoResearch Laboratories (Jackson ImmunoResearch Laboratories, Cambridgeshire, UK). Western blot reagents (membranes, gels and protein marker) were purchased from Bio-Rad (Bio-Rad Laboratories Ltd., Watford, Hertfirdshire, UK).