Autosampler

Manufactured by Bruker

Sourced in Germany, United States, Canada

The Autosampler is a lab equipment product designed to automatically introduce samples into an analytical instrument, such as a spectrometer or chromatograph, for analysis. It enables hands-free, high-throughput sample processing, improving efficiency and reproducibility.

Automatically generated - may contain errors

Lab products found in correlation

Avance 3 600 mhz spectrometer, by Bruker (1 mentions) Wx vortex, by VELP Scientifica (1 mentions) R 114 rotary evaporator, by Büchi (1 mentions) Fused silica capillary column, by Merck Group (1 mentions) Sigmaultra, by Merck Group (1 mentions) Tensor 37, by Bruker (1 mentions) Opus software, by Bruker (1 mentions) Hts xt, by Bruker (1 mentions)

4 protocols using autosampler

Metabolic Profiling of Algae under pH Stress

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Frozen algal samples were divided into four portions of about 1 g each to obtain four technical replicates for the two experimental conditions (pH 8.1—control vs. pH 7.7—treatment). Briefly, 170 μL of water and 700 μL of methanol (MeOH) were added, and the samples were sonicated for 30 s. Then, 350 μL of chloroform was added, and after vortexing and shaking for 10 min, 350 μL of water and 350 μL of CHCl₃ were added. After centrifugation at 10,000 g for 10 min at 4 °C, the upper phase containing polar metabolites was collected, lyophilized, and stored at −80 °C until analysis.
NMR analyses were performed on a Bruker Avance III–600 MHz spectrometer with an autosampler (Bruker BioSpin GmbH, Rheinstetten, Germany), equipped with a TCI CryoProbeTM fitted with a gradient along the Z-axis, at a probe temperature of 27 °C. Details for 1D and 2D experiments [127 (link),128 (link),129 (link),130 (link),131 (link),132 (link)] are reported in the Supplementary Materials. Metabolites were assigned to NMR profiles by comparison of chemical signal shifts (¹H and ¹³C nuclei) with literature data [133 (link)] and/or an online database [134 (link)].

Sanchez-Arcos C., Paris D., Mazzella V., Mutalipassi M., Costantini M., Buia M.C., von Elert E., Cutignano A, & Zupo V. (2022). Responses of the Macroalga Ulva prolifera Müller to Ocean Acidification Revealed by Complementary NMR- and MS-Based Omics Approaches. Marine Drugs, 20(12), 743.

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Extraction and Analysis of MOH

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To extract MOH from the samples, a WX vortex from Velp Scientifica (Usmate, Italy) was utilized. An R-114 rotary evaporator from Büchi (Flawil, Switzerland) was used for the evaporation of the solvent.
A Scion GC system equipped with an autosampler (Bruker Corporation, Freemont, CA, USA) was used for chromatographic analyses. An ultra-inert liner SPI 0.25/0.32 mm from Agilent was used to simulate on-column injection. A DB-1HT capillary column (15 m × 0.32 mm i.d. × 0.10 µm film thickness) from Agilent (Santa Clara, CA, USA) was utilized for GC separation after an untreated fused silica capillary column used as pre-column (2 m × 0.32 mm) from Supelco (Bellefonte, PA, USA). The two columns were connected with a press-fit column connection from Agilent. Helium was used as carrier gas at a constant flow rate of 3 mL/min (62.2 cm/s linear velocity). Interactive Graphics (Bruker) v8.2.1 software was used for optimization and quantification.

Ruiz J.L., Liébanas J.A., Vidal J.L., Garrido Frenich A, & González R.R. (2021). Offline Solid-Phase Extraction and Separation of Mineral Oil Saturated Hydrocarbons and Mineral Oil Aromatic Hydrocarbons in Edible Oils, and Analysis via GC with a Flame Ionization Detector. Foods, 10(9), 2026.

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Synovial Fluid Analysis via FTIR

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Synovial fluid samples were thawed at 22 °C and dried-films were prepared as described previously with the following modifications [19 ]. An internal spectroscopic control of potassium thiocyanate (KSCN, SigmaUltra, Sigma-Aldrich Inc, St Louis, MO) in a 2:1 SF to KSCN ratio (40:20 μL) was used. Six, 8 μL replicates for each sample was applied to 96-well silicon microplates [19 ,34 ]. Each microplate was dried at room temperature (20–22 °C) and then placed in the multi-sampler (HTS-XT, Autosampler, Bruker Optics, Milton, ON, Canada) attachment of an IR spectrometer (Tensor 37, Bruker Optics). Infrared absorbance spectra in the wavenumber range of 400 to 4000 cm⁻¹ were recorded using the OPUS software (version 6.5, Bruker Optics, GmbH, Ettlingen, Germany). For each sample evaluation, 512 interferograms were signal averaged and were Fourier transformed to produce a nominal resolution of 4 cm⁻¹ for the resulting spectrum [20 (link),21 (link),34 ].

Malek S., Marini F., Rochat M.C., Béraud R., Wright G.M, & Riley C.B. (2020). Infrared spectroscopy of synovial fluid as a potential screening approach for the diagnosis of naturally occurring canine osteoarthritis associated with cranial cruciate ligament rupture. Osteoarthritis and Cartilage Open, 2(4), 100120.

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Thermal Desorption for Removal of Releasable TCA in Cork Stoppers

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To evaluate the efficiency of thermal desorption on naturally contaminated stoppers, the releasable TCA was determined in the stoppers before they were subjected to a thermal process in vacuum.
Complying with the ISO 20752:2014 standard [10 ], each stopper was individually soaked in a 12% (v/v) hydroalcoholic solution for 24 ± 2 h. Then, a 10 mL sample was collected for analysis. The test portion was saturated with sodium chloride (NaCl, purity ≥ 99.5%), and 2,3,6-trichloroanisole (Neochema, purity ≥ 99.0%) was used as the internal standard. Ethanol had a minimum purity of 96%, and pure deionized water without TCA was used. Releasable TCA was measured by SPME-GC (solid-phase microextraction followed by gas chromatography) with an electron-capture detector (ECD) Bruker 450-GC equipped with a Combipal autosampler. Further details used for the quantification of the releasable TCA can be found in [5 (link)].
After selecting 215 cork stoppers with a releasable TCA concentration exceeding 0.5 ng/L, they were subjected to an 8 h processing period at temperatures exceeding 150 °C within a rotating drum connected to a double-stage rotary vane pump. This time was needed to reach the cork beyond its surface. The minimal pressure achieved was approximately 0.1 mbar. Then, the releasable TCA in each stopper was determined as explained before.

Monteiro S., Bundaleski N., Lopes P., Cabral M, & Teodoro O.M. (2023). Thermal Desorption of 2,4,6-Trichloroanisole from Cork. Foods, 12(18), 3450.

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