D3 25ohd3

Manufactured by Merck Group

Sourced in Panama

D3-25OHD3 is a reference standard used for the quantitative determination of 25-hydroxyvitamin D3 in biological samples. It serves as a calibrator or quality control material to ensure the accuracy and reliability of analytical methods for measuring 25-hydroxyvitamin D3 levels.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Optima grade water, by Thermo Fisher Scientific (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) C3epi, by Merck Group (2 mentions) Labostar twf uv 7, by Siemens (1 mentions) Formic acid, by Merck Group (1 mentions) Ammonium formate, by Merck Group (1 mentions) 4 phenyl 1 2 4 triazoline 3 5 dione ptad, by Merck Group (1 mentions) Hplc grade dichloromethane, by Thermo Fisher Scientific (1 mentions)

3 protocols using d3 25ohd3

Quantification of 25OHD3 using HPLC-MS/MS

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Optima grade water, acetonitrile, methanol, hexane, HPLC grade dichloromethane, and 99% formic acid were obtained from Fisher Scientific (Pittsburgh, PA). 25OHD₃ was obtained from Isosciences (King of Prussia, PA) and d3-25OHD₃, C3epi, and PTAD were obtained from Sigma Aldrich (St. Louis, MO).

Teegarden M.D., Riedl K.M, & Schwartz S.J. (2015). Chromatographic separation of PTAD-derivatized 25-hydroxyvitamin D3 and its C-3 epimer from human serum and murine skin. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 991, 118-121.

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Quantitative Analysis of Vitamin D Metabolites

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VD3, 25(OH)D3, isotopically labeled 6, 19, 19 [d3]-VD3, d3–25(OH)D3, methyl tert-butyl ether (MTBE), formic acid (LC-MS grade), and ammonium formate (≥99.0%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, ethanol, and acetonitrile (AcN) were liquid chromatography/mass spectrometry (LC/MS)-grade and purchased from Fisher Scientific (Pittsburgh, PA, USA). Ultra-pure water was prepared with LaboStar™ TWF UV 7 (Siemens, MA, USA).

Park C.Y., Shin Y., Kim J.H., Zhu S., Jung Y.S, & Han S.N. (2020). Effects of high fat diet-induced obesity on vitamin D metabolism and tissue distribution in vitamin D deficient or supplemented mice. Nutrition & Metabolism, 17, 44.

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Vitamin D Metabolite Quantification

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Optima grade water, acetonitrile, methanol, hexane, HPLC grade dichloromethane, and 99% formic acid were obtained from Fisher Scientific (Pittsburgh, PA). 25OHD3 was obtained from Isosciences (King of Prussia, PA) and d3-25OHD3, C3epi, and 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) were obtained from Sigma Aldrich (St. Louis, MO).

Teegarden M.D., Campbell A.R., Cooperstone J.L., Tober K.L., Schwartz S.J, & Oberyszyn T.M. (2017). 25-Hydroxyvitamin D3 and its C-3 epimer are elevated in the skin and serum of Skh-1 mice supplemented with dietary vitamin D3. Molecular nutrition & food research, 61(10), 10.1002/mnfr.201700293.

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