Human NSCLC cells including A549 and H1650 were purchased from Procell Biotechnology (Wuhan, China). A549 and H1650 were cultured according to the manufacturer's instruction. Brie y, A549 and H1650 cells were cultured in RPMI1640 (Thermo Fisher, Catalog number: 61870036) supplemented with 10% exosomes-free FBS (Thermo Fisher, Catalog number: 10082147) at 37 °C incubators containing 5% CO2. Cells were sub-cultured when con uence reaches more than 90%.
H1650
Manufactured by Procell
Sourced in China
The H1650 is a compact and versatile laboratory equipment designed for general scientific purposes. It features a sturdy construction and a user-friendly interface. The core function of the H1650 is to assist in various laboratory tasks, though its specific applications may vary depending on the needs of the user.
Automatically generated - may contain errors
Lab products found in correlation
H1650, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
H1299, by Procell (2 mentions)
Pm150210, by Procell (1 mentions)
Pm150910, by Procell (1 mentions)
Pm150110, by Procell (1 mentions)
Pb180120, by Procell (1 mentions)
Hek293t cells, by Procell (1 mentions)
Dulbecco modified eagle medium (dmem), by Procell (1 mentions)
Fetal bovine serum (fbs), by Procell (1 mentions)
Ham s f12k, by Procell (1 mentions)
Penicillin streptomycin, by Sartorius (1 mentions)
H1975, by Procell (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
5 protocols using h1650
1
Cell Culture of NSCLC Lines
2
Culturing Human Lung Cell Lines
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Human bronchial epithelial cells (HBE), HEK-293 T cells, and lung cancer cells (H460, H1650, A549, and H1299) (Procell, Wuhan, China) were maintained under the right conditions (5% carbon dioxide and 37°C). HEK-293 T and A549 cells were, respectively, cultured in DMEM (PM150210, Procell) and Ham’s F-12 K (PM150910, Procell), whereas other cells were cultured in Roswell Park Memorial Institute-1640 Medium (PM150110, Procell). All media utilized for cell growth were supplemented with 10% Fetal Bovine Serum (FBS) (164,210–500, Procell) and 1% Penicillin/Streptomycin (PB180120, Procell).
3
NSCLC Cell Culture Conditions
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MRC‐5 human embryonic lung fibroblasts, MRC‐5 culture medium, and the NSCLC cell lines H1299, H1975, A549, H1650, and PC9 were obtained from Procell Life Science and Technology. A549 and PC9 cells were cultured in high‐glucose Dulbecco's modified Eagle's medium (Hyclone) containing 10% fetal bovine serum (FBS; Biological Industries) and 1% penicillin/streptomycin (Biological Industries), whereas other NSCLC cell lines were grown in RPMI‐1640 medium (Hyclone) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were incubated in a humidified incubator with 5% CO2 at 37°C.
4
Cell Culture of LUAD Cell Lines
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LUAD cells (A549 and H1650) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). A549 cells were cultured in Ham’s F-12K medium supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin-streptomycin. H1650 cells were maintained in RPMI-1640 medium containing 10% FBS and 1% P/S. The cells were cultured in a humidified atmosphere of 5% CO2 at 37°C.
5
Culturing Human NSCLC Cell Lines
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Human NSCLC cells including A549 and H1650 were purchased from Procell Biotechnology (Wuhan, China). A549 and H1650 were cultured according to the manufacturer's instructions. Briefly, A549 and H1650 cells were cultured in RPMI1640 (Thermo Fisher, Catalog number: 61870036) supplemented with 10% exosomes-free FBS (Thermo Fisher, Catalog number: 10082147) at 37°C incubators containing 5% CO2. Cells were subcultured when confluence reaches more than 90%.
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