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Biotinylated anti mouse il 10 sxc 1

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Biotinylated anti-mouse IL-10 SXC-1 is a laboratory reagent used in immunological research. It is an antibody that has been conjugated with biotin, a small molecule that can be easily detected. This product is designed for use in various immunoassay techniques, such as ELISA, to measure the levels of the cytokine interleukin-10 (IL-10) in mouse-derived samples.

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Lab products found in correlation

Ifn β, by PBL Assay Science (2 mentions) Hrp conjugated streptavidin, by Jackson ImmunoResearch (2 mentions) Cone jes5 2a5, by Thermo Fisher Scientific (2 mentions) Il 1β, by R&D Systems (2 mentions) Il 12p70, by Thermo Fisher Scientific (1 mentions)

2 protocols using biotinylated anti mouse il 10 sxc 1

1

Cytokine Quantification by ELISA

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Cytokines were quantified from supernatants of stimulated cells. IL-10 and IL-12p40 were quantified by ELISA. Cone JES5-2A5 (eBioscience) was used for IL-10 capture, and biotinylated anti-mouse IL-10 SXC-1 (BD Biosciences) was used for detection. Clone C15.6.7 was used for IL-12p40 capture, and biotinylated anti-mouse IL-12p40 C17.8 was used for detection (both gifts from DNAX Research Institute), followed by HRP-conjugated streptavidin (Jackson ImmunoResearch Laboratories). IL-12p70, TNF-α, and IL-27 (eBioscience), IL-1β (R&D Systems), and IFN-β (PBL) were quantified using commercially available ELISA kits.
Howes A., Taubert C., Blankley S., Spink N., Wu X., Graham C.M., Zhao J., Saraiva M., Ricciardi-Castagnoli P., Bancroft G.J, & O’Garra A. (2016). Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages. The Journal of Immunology Author Choice, 197(7), 2838-2853.
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2

Cytokine Quantification from Stimulated Cells

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Cytokines were quantified from supernatants of stimulated cells. IL-10 and IL-12p40 were quantified by ELISA. Cone JES5-2A5 (eBiosciences) was used for IL-10 capture and biotinylated anti-mouse IL-10 SXC-1 (BD Biosciences) for detection. Clone C15.6.7 was used for IL-12p40 capture and biotinylated anti-mouse IL-12p40 C17.8 for detection (both gifts from DNAX), followed by HRP conjugated Streptavidin (Jackson ImmunoResearch Laboratories Inc.). IL-12p70, TNF-α and IL-27 (eBioscience), IL-1β (R&D) and IFN-β (PBL) were quantified using commercially available ELISA kits.
Howes A., Taubert C., Blankley S., Spink N., Wu X., Graham C.M., Zhao J., Saraiva M., Ricciardi-Castagnoli P., Bancroft G.J, & O’Garra A. (2016). Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages. The Journal of Immunology Author Choice, 197(7), 2838-2853.
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