Digital sight ds 2mv camera

Manufactured by Nikon

Sourced in Japan

The Digital Sight DS-2Mv is a digital camera designed for laboratory and microscopy applications. It features a CMOS sensor with a resolution of 2 megapixels and supports a wide range of resolutions and frame rates. The camera can be connected to a computer via a USB interface for image capture and display.

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Lab products found in correlation

Nis elements d 3, by Nikon (2 mentions) Optiphot 2 microscope, by Nikon (1 mentions) Total starch kit, by Megazyme (1 mentions) Eclipse ti u inverted microscope, by Nikon (1 mentions) Nis elements ar 3, by Nikon (1 mentions) Smz800 dissecting microscope, by Nikon (1 mentions) Polysine adhesion slide, by Thermo Fisher Scientific (1 mentions) Eclipse 50i microscope, by Nikon (1 mentions) Api 20c aux strips, by bioMérieux (1 mentions) Eclipse 80, by Nikon (1 mentions) Labophot 2 microscope, by Nikon (1 mentions)

Close spelling variants of this product

Digital camera (372 citations) Digital Sight camera (50 citations) DS-2Mv camera (12 citations) Digital Sight DS-2Mv (11 citations) DS-2Mv (11 citations) DS-2Mv Nikon digital camera (8 citations) Digital-Sight DS-2Mv digital camera (3 citations)

8 protocols using digital sight ds 2mv camera

Starch Content Analysis and Visualization

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Analysis of total starch content was performed using the Total Starch kit from Megazyme. Protocol K‐TSTA 09/14 method a was followed.
For visual confirmation of the presence of starch, whole hulls from batches Swe17 and Swe18 were taken. A drop of undiluted Lugol reagent (Merck) was added to either the interior or exterior side of the hull for 3 min. Imaging was performed using a Nikon OPTIPHOT‐2 microscope equipped with a Plan 20/0.50 DIC 160/0.17 objective and a Nikon Digital Sight DS‐2 Mv camera. The software used for image analysis was NIS‐Elements D 3.1 (Nikon, Tokyo, Japan).

Schmitz E., Nordberg Karlsson E, & Adlercreutz P. (2020). Warming weather changes the chemical composition of oat hulls. Plant Biology (Stuttgart, Germany), 22(6), 1086-1091.

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Adsorption of Prion Protein by Hyaluronic and Formic Acids

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Two MoPrP concentrations (25 and 60 µg/mL) were incubated with 5 and 20 µg/mL of HA or FA in 50 µL volume at 22°C for 6 hours. All the samples were prepared by drop casting on a surface of freshly cleaved muscovite mica and left to adhere till total solvent evaporation. All AFM measurements were performed on a standard MFP-3D stand alone Asylum Research AFM (Oxford Instruments, UK) in dynamic mode using commercially available silicon probes (NSG30 cantilever characterized by a spring constant of about 40 nN/nm, resonance frequency of about 340 kHz and tip radius less than 6 nm). Images were acquired in air at a resolution of 512×512 pixels at 1 Hz scan speed. Optical microscopy characterization was performed on a Nikon Eclipse Ti-U inverted microscope. Phase images were recorded with a Nikon Digital Sight DS-2 Mv camera at 1600×1200 pixels resolution on a 20× objective.

Giachin G., Narkiewicz J., Scaini D., Ngoc A.T., Margon A., Sequi P., Leita L, & Legname G. (2014). Prion Protein Interaction with Soil Humic Substances: Environmental Implications. PLoS ONE, 9(6), e100016.

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Fluorescence Imaging of GFP-Labeled Specimens

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Low magnification (1–2×) bright-field and GFP images were captured with Nikon Digital Sight DS-2Mv camera attached to a SMZ800 dissecting microscope (Nikon, Japan). Illumatool Bright Light Systems LT 9900 (Lightools Research) with GFP filter set (470 nm excitation and 515 nm emission) was placed under the objective of the microscope to visualize GFP fluorescence. High magnification images were obtained using Nikon Eclipse 80i upright microscope and processed using NiS Elements AR3.0 software (Nikon, Japan). All image manipulations (merging, brightness and contrast adjustment) were performed using ImageJ Version 1.49a (NIH, US).

Bazhanov N., Ylostalo J.H., Bartosh T.J., Tiblow A., Mohammadipoor A., Foskett A, & Prockop D.J. (2016). Intraperitoneally infused human mesenchymal stem cells form aggregates with mouse immune cells and attach to peritoneal organs. Stem Cell Research & Therapy, 7, 27.

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Aortic Fatty Streak Quantification in Mice

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Hearts of 28-week-old KRIT1^+/− and wild-type C57BL/6 mice following 22 weeks of HF diet were snap-frozen and cut on a Leica (Wetzlar, Germany) cryostat into 10-μm sections, starting at the apex until it reached the aortic valve area. Two samples from wild-type mice and one sample from KRIT1^+/− mice were discarded due to the poor quality of tissue. At least four consecutive sections were fixed in Baker’s fixative, stained in Oil Red O and hematoxylin solution, and mounted on glass slides. Images were acquired by Nikon Digital Sight DS-2Mv camera coupled to a light inverted microscope (4× objective). Percentage of cross-sectional aortic area with fatty streaks deposition was quantified by the ImageJ software (ImageJ analysis software: http://imagej.nih.gov/ij/).

Vieceli Dalla Sega F., Mastrocola R., Aquila G., Fortini F., Fornelli C., Zotta A., Cento A.S., Perrelli A., Boda E., Pannuti A., Marchi S., Pinton P., Ferrari R., Rizzo P, & Retta S.F. (2019). KRIT1 Deficiency Promotes Aortic Endothelial Dysfunction. International Journal of Molecular Sciences, 20(19), 4930.

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Membrane Permeabilization in C. albicans

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The potential role of ISDPs in membrane permeabilization in living C. albicans SC5314 cells was studied by fluorescence microscopy using a Nikon Eclipse 80i optical microscope, equipped with a Nikon Digital Sight DS-2Mv camera, and images were acquired with NIS Elements F control software (Nikon Co., Tokyo, Japan). Yeast cells grown in yeast extract, peptone, and dextrose broth overnight at 30 °C with shaking (100 rpm) were washed once with water and 4 × 10⁷ cells/mL were loaded with 500 μM Lucifer Yellow (LY) and 1.5 μM propidium iodide (PI). LY is a fluorescent molecule used as a quantitative marker of the cell membrane permeabilization [23 (link)], while PI is a non-vital nuclear stain commonly used for identifying dead cells. Yeast cell suspensions (10 μL) were seeded on Polysine Adhesion Slides (Thermo Scientific™, Thermo Fisher Scientific Inc., Waltham, MA, USA) and, after 10 min, ISDPs were added (final concentrations in the range 90–97 μM). Images were taken up to 30 min at a 40× magnification.

Ciociola T., Magliani W., De Simone T., Pertinhez T.A., Conti S., Cozza G., Marin O, & Giovati L. (2021). In Silico Predicted Antifungal Peptides: In Vitro and In Vivo Anti-Candida Activity. Journal of Fungi, 7(6), 439.

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Fungal Identification and Characterization

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Thirty-nine clinical isolates were re-examined by macroscopic and microscopic studies according to Marimon criteria [12 (link)]. Macroscopic characteristics of colonies were studied by culturing isolates on potato dextrose agar (PDA); plates were incubated at 30, 35, and 37 °C. Petri dishes were inoculated with colonies of each fungus and 10 μL of conidial suspension adjusted to 1 × 10⁷ cells/mL for each isolate. After 14 and 21 days, colony diameters were measured in duplicate. Microscopic features of conidia were determined by slide cultures made on PDA after 12–15 days of incubation in the dark and in a humid chamber. Coverslips were mounted in lactophenol cotton blue. The slides were examined under a Nikon Eclipse 50i microscope fitted with a Nikon digital sight DS-2Mv camera. The conidia were measured. The ability of all isolates to reverse to yeast-like cells at 37 °C was performed on brain heart infusion (BHI) with blood for 9 days. Carbohydrate assimilation was tested using API 20C AUX strips (bioMerieux, Mexico City, Mexico) [13 (link)].

Rojas O.C., Bonifaz A., Campos C., Treviño-Rangel R.D., González-Álvarez R, & González G.M. (2018). Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico. Journal of Fungi, 4(3), 86.

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Histological and Antigen Assessment of Arteriovenous Fistula

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Histology and antigen detection was performed using light microscope (Nikon Eclipse 80, Düsseldorf, Germany) at Â400 magnification and Nikon NIS-Elements D 3.0 software; pictures were taken using a Nikon DIGITAL SIGHT DS-2Mv camera.
Intimal and medial thickness was measured in AVF-vein and vena femoralis using one section per level (L1-L6) (Supplementary Figure S1 online) and one section from the contralateral vein. Twenty measurements for each level were performed; averaged mean width and percentage values for intima and media were calculated for every group. 12 In addition, intima/media ratios were calculated out of vessel layer width of intima and media. 12 An identical procedure was performed in human samples; additionally estimating mean values out of several blocks per sample. However, level division depending on the location of the vein sample relating to the anastomosis was unfeasible in human samples.
Qualitative evaluation of calcification was performed in vKstained sections as described before. 12 Assessment was performed on each level of AVF-vein and on vena femoralis and on one human vein section per paraffin block.

Zaragatski E., Grommes J., Schurgers L.J., Langer S., Kennes L., Tamm M., Koeppel T.A., Kranz J., Hackhofer T., Arakelyan K., Jacobs M.J, & Kokozidou M. (2016). Vitamin K antagonism aggravates chronic kidney disease-induced neointimal hyperplasia and calcification in arterialized veins: role of vitamin K treatment?. Kidney international, 89(3).

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Protist Cytology and Identification Protocol

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We analyzed each one of the 60 samples 48 h after sampling and at intervals of two days through the next two weeks. We observed cytological characters of taxonomical importance in vivo (e.g., cell size and shape, position and shape of the cytostome, position and number of the contractile vacuoles) using bright field and differential interference contrast microscopy with a Nikon Labophot-2 microscope equipped (Tokyo, Japan) with a Nikon Digital Sight DS-2Mv camera (Tokyo, Japan). Additionally, we established cul-tures in Petri dishes using some drops of the original sample, table water Evian ® (Haute Savoie, France) and wheat grains to stimulate bacterial growth [9] (link). We performed silver impregnation techniques following the protocols of Foissner [26] (link) to reveal oral and somatic infraciliature, number and disposition of cirri, the Silverline pattern and nuclear apparatus. We followed Foissner [27] [28] [29] [30] [31] (link)[32] , Foissner et al. [33] (link), Guggiari and Peck [34] (link), Lee et al. [35] , Omar and Foissner [36, 37] (link) and Penard's studies [38] for species identification. Systematics was according to Lynn [12] .

Durán-Ramírez C.A., & Mayén‐Estrada R. (2022). Ecological Study of the Active Ciliate Community from Bromeliads during the Humid Season in Different Types of Forests of the Mexican Neotropics. Diversity, 14(2), 122-122.

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