Chitosan (DD > 90%) was purchased from Qingdao Biotemed Biomaterial Co., Ltd. (Qingdao, China). Chitosanase was prepared, purified, and identified in our laboratory. Fetal bovine serum (FBS) and RPMI 1640 medium were obtained from Gibco®, Life Technologies (Carlsbad, CA, USA). Calcein-AM was purchased from Fanbo Biochemical Co., Ltd. (Beijing, China). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and propidium iodide (PI) were obtained from Sigma–Aldrich (St. Louis, MO, USA). The RIPA lysis buffer and the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel preparation kit were obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). The 3,3-diaminobenzidine (DAB) reagent kit, mouse tumor necrosis factor α (TNF-α) enzyme-linked immune sorbent assay (ELISA) kit, Mouse interleukin 2 (IL-2) ELISA Kit, Mouse Fas ELISA Kit and the Mouse Fas-L ELISA Kit were purchased from Boster Biological Engineering Co., Ltd. (Wuhan China). The rabbit polyclonal anti-Bcl-2 antibody, rabbit polyclonal anti-Bax antibody and goat anti-rabbit IgG Horse Radish Peroxidase (HRP) were purchased from Abcam Inc (Cambridge, MA, USA). Polyvinylidene difluoride (PVDF) membranes and bovine serum albumin (BSA) were obtained from Solarbio Science and Technology Co., Ltd. (Beijing, China).
Sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page gel preparation kit
Manufactured by Beyotime
Sourced in China
The Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel preparation kit is a laboratory equipment used to separate and analyze proteins based on their molecular weight. The kit contains the necessary components to prepare polyacrylamide gels for protein electrophoresis.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Polyvinylidene difluoride pvdf membranes, by Solarbio (1 mentions)
Goat anti rabbit igg horseradish peroxidase hrp, by Abcam (1 mentions)
Rabbit polyclonal anti bcl 2 antibody, by Abcam (1 mentions)
Rabbit polyclonal anti bax antibody, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bovine serum albumin (bsa), by Solarbio (1 mentions)
Rabbit anti rat β actin polyclonal antibody, by Abcam (1 mentions)
Rabbit anti rat nrf2 polyclonal antibody, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Enhanced chemiluminescence reagent kit, by Beyotime (1 mentions)
Sodium hydrosulfide nahs, by Merck Group (1 mentions)
Bicinchoninic acid assay bca protein assay kit, by Beyotime (1 mentions)
4 protocols using sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page gel preparation kit
1
Chitosan-based Cytotoxicity Evaluation
2
Nrf2, HO-1, NF-κB, and TNF-α Protein Expression
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RA (specification: 1 g), SFN (specification: 5 mg), and dimethyl sulfoxide (DMSO) (specification: 100 mL/bottle) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). The following primary antibodies were used: rabbit anti-rat Nrf2 polyclonal antibody and goat anti-rat HO-1 polyclonal antibody purchased from Abcam (Cambridge, UK); and rabbit anti-rat NF-κB polyclonal antibody and rabbit anti-rat tumor necrosis factor (TNF-α) polyclonal antibody purchased from Cell Signaling Technology (Beverly, MA, USA). The following secondary antibodies were used: dog anti-rabbit polyclonal antibody labeled with Cy3 fluorescein and dog anti-goat polyclonal antibody labeled with fluorescein isothiocyanate, which were purchased from Abcam; and rabbit anti-rat β-actin polyclonal antibody, which was purchased from Abcam. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) gel preparation kit was purchased from Beyotime Institute of Biotechnology (Shanghai, People’s Republic of China). Instruments included the following: animal skull stereotaxic apparatus, electronic analytical balance, image analyzer, and ultraviolet spectrophotometer.
3
Apricot Kernel Protein Extraction and Characterization
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Apricot kernels were purchased from the Northwest Herb Market of Xi'an (Shaanxi Province, China). The Bicinchoninic Acid (BCA) protein assay kit (50T) was purchased from Solar Co. Ltd. (Beijing, China). 8-aniline-1-naphthalene sulfonic acid was bought from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel preparation kit, electrophoresis solution, protein loading buffer (5 × ), color pre-stained protein molecular weight standard, horseradish peroxidase-labeled goat anti-rabbit lgE, and Efficient chemiluminescence (ECL) kit (Enhanced) were purchased from the Beyotime Biotech Inc. (Shanghai, China). Rabbit-derived amandin polyclonal antibody was bought from Wuhan Youyouda Biotechnology Co. Ltd. (Wuhan, Hubei Province, China). All other chemicals were of analytical grade.
4
Sodium Hydrosulfide Attenuates Diabetic Endoplasmic Reticulum Stress
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Fifty adult male Sprague-Dawley (SD) rats, weighing 280-300 g, were purchased from the SJA Lab Animal Center of Changsha (Changsha, China). The animals were maintained in accordance with institutional policies, and all experiments were performed with approval of the University of China Committee on the Use and Care of Animals of University of South China (Hengyang, China).
Drugs and reagents. Sodium hydrosulfide (NaHS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Streptozotocin (STZ) was purchased from MP Biomedicals Company (Santa Ana, CA, USA). Specific monoclonal anti-GRP78 antibody was purchased from Epitomics Inc. (Burlingame, UK). Specific monoclonal anti-CHOP antibodies were purchased from Proteintech Group, Inc. (Chicago, IL, USA). Specific monoclonal anti-caspase-12 antibodies were obtained from Sigma-Aldrich. Anti-rabbit and anti-rat IgG secondary antibodies were purchased from Proteintech Group, Inc. Cell lysis buffer for western blot analysis, Enhanced Chemiluminescence Reagent kit, Bicinchoninic acid assay (BCA) Protein Assay kit and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Gel Preparation kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China).
Drugs and reagents. Sodium hydrosulfide (NaHS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Streptozotocin (STZ) was purchased from MP Biomedicals Company (Santa Ana, CA, USA). Specific monoclonal anti-GRP78 antibody was purchased from Epitomics Inc. (Burlingame, UK). Specific monoclonal anti-CHOP antibodies were purchased from Proteintech Group, Inc. (Chicago, IL, USA). Specific monoclonal anti-caspase-12 antibodies were obtained from Sigma-Aldrich. Anti-rabbit and anti-rat IgG secondary antibodies were purchased from Proteintech Group, Inc. Cell lysis buffer for western blot analysis, Enhanced Chemiluminescence Reagent kit, Bicinchoninic acid assay (BCA) Protein Assay kit and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Gel Preparation kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China).
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