Lumi light plus western blotting kit

Endothelial Cell Growth Medium (cat. C-22010), penicillin/streptomycin solution, trypsin-EDTA solution, Lumi-Light PLUS Western Blotting Kit (including anti-mouse and anti-rabbit IgG-HRP secondary antibodies), protease and phosphatase inhibitor cocktails, extravidin-alkaline phosphatase (AP) conjugate, and 1-deoxymannojirimycin (DMJ) were purchased from Merck Group (Darmstadt, Germany). RPMI 1640 GlutaMAX™-I medium, DMEM medium, foetal bovine serum (FBS), Alamar Blue cell viability reagent, and MicroBCA Protein Assay kit were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The 2x concentrated Laemmli Buffer was obtained from Bio-Rad Laboratories (Hercules, CA, USA). Biotinylated lectins: Phaseolus vulgaris leukoagglutinin (PHA-L), Phaseolus vulgaris erythroagglutinin (PHA-E), Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), Galanthus nivalis agglutinin (GNA), and Aleuria aurantia agglutinin (AAA) were purchased from Vector Laboratories Inc. (Burlingame, California, United States). ApogeeMix reference beads were purchased from Apogee Flow Systems (Northwood, UK). Polyvinyidene fluoride membranes (PVDF) were from Millipore (Burlington, MA, USA). All remaining chemicals were of analytical grade, commercially available.

RPMI 1640 GlutaMAX™-I medium, fetal bovine serum (FBS), Alamar Blue cell viability reagent, and MicroBCA protein assay kit were obtained from Life Technologies (Carlsbad, CA, USA). Lumi-LightPLUS Western blotting kit (including anti-mouse IgG-HRP secondary antibody), trypsin–EDTA solution, and penicillin–streptomycin solution were purchased from Merck Group (Darmstadt, Germany). The 10% TGX SDS–PAGE stain-free precast gels and 2× concentrated Laemmli buffer were the products of Bio-Rad Laboratories (Hercules, CA, USA). LC–MS grade solvents were supplied by Supelco (Bellefonte, PA, USA). Other chemicals were of analytical grade and commercially available.

RPMI 1640 GlutaMAX™-I medium, fetal bovine serum (FBS), MicroBCA Protein Assay kit, Alamar Blue cell viability reagent, Acclaim PepMap trap (100 C18, 75 μm × 20 mm, 3 μm particle, 100 Å pore size) and analytical (Acclaim PepMap RSLC C18, 75 µm × 500 mm, 2 µm particle, 100 Å pore size) columns were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-CD63 mouse monoclonal primary antibody (clone RFAC4, cat. CBL553), Lumi-LightPLUS Western Blotting Kit (including anti-mouse IgG-HRP secondary antibody), Trypsin-EDTA solution, penicillin/streptomycin solution, SpeedBeads™ GE45152105050250 and GE65152105050250, and HEPES buffer were obtained from Sigma-Aldrich (St. Louis, MO, USA). Mouse monoclonal primary antibodies for Arf6 (clone 3A-1, cat. sc-7971) and HSP70 (clone C92F3A-5, cat. sc-66048) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Trypsin/Lys-C Mix was the product of Promega (Madison, WI, USA). All remaining chemicals were of analytical grade, commercially available.