HO-3867 (cat no: S7501), DFO (cat no: S6849), Dp44mT (cat no: S790), dexrazoxane HCl (cat no: S5651), salinomycin (cat no: S8129), ebselen (cat no: S6676), z.VAD.FMK (cat no: S7023), Nec-1 (cat no: S8037), Fer-1 (cat no: S7243), Lip-1 (cat no: S7699), and PFTα (cat no: S2929) were procured from Selleck Chemicals (USA). S-Benzylisothiourea (cat no: S9138) was ordered from Sigma-Aldrich (USA). The primary antibodies listed below were ordered from Cellular Signaling Technology (USA): caspase-3 (cat no:9662), Mcl-1 (cat no:94296), Bcl-2 (cat no:15071), Bcl-xL (cat no:2762), Bak (cat no:6947), Bax (cat no:94296), and DMT1 (cat no:15083). The following primary antibodies listed were procured from Abcam (USA): FPN1 (cat no: ab239583), TFR (cat no: ab214039), FTL (cat no: ab75973), FTH (cat no: ab183781), and p53 (cat no: ab26). The secondary antibody was procured from Abclonal (China). Sigma-Aldrich supplied all other routine chemicals.
Dp44mt
Manufactured by Selleck Chemicals
Sourced in United States
Dp44mT is a chemical compound used as a laboratory reagent. It functions as a chelating agent, capable of binding to and sequestering metal ions. The specific details and intended applications of Dp44mT may vary depending on the context of its use in research or scientific experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Nec 1, by Selleck Chemicals (1 mentions)
Ebselen, by Selleck Chemicals (1 mentions)
Salinomycin, by Selleck Chemicals (1 mentions)
Lip 1, by Selleck Chemicals (1 mentions)
Ho 3867, by Selleck Chemicals (1 mentions)
Fer 1, by Selleck Chemicals (1 mentions)
Z vad fmk, by Selleck Chemicals (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by ScienCell (1 mentions)
Hepatocyte growth supplement, by ScienCell (1 mentions)
2 protocols using dp44mt
1
Pharmacological Modulation of Cell Death
2
Iron Chelator-Based Cell Culture Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Huh7 and HepG2 cells were cultured in high glucose DMEM (GIBCO, CA, USA); SMMC-7721 cells and H22 cells were cultured in RPMI-1640, both containing 10% fetal bovine serum (GIBCO, CA, USA). Human and mouse primary hepatocytes were cultured in hepatocyte medium with addition of 10% fetal bovine serum and hepatocyte growth supplement (ScienCell, CA, USA). All primary cells were purchased from ScienCell (CA, USA) with STR Authentication. Iron chelators DFO (Cat#. S6849) and Dp44mT (Cat#. S7909) were purchased from Selleck Chemicals (TX, USA). Lipofectamine 3000 (Thermo Fisher, USA) was used to transfect siRNA or plasmid for cells as previously described.39 (link) The Phen Green FL reagent for fluorescence quenching assay was purchased from Thermo Fisher, USA
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