Aspartate aminotransferase

To assess the hepatocellular injury following CLP, alanine aminotransferase (ALT), aspartate aminotransferase (AST) (Pars Azmoon Co, Iran), alkaline phosphatase (ALP) (Ziest Chem Diagnostics Co, Iran) and total bilirubin (BILI) (Darman Faraz Kave Co, Iran) were measured spectrophotometrically in serum according to the procedure described in the kit purchased.

Blood samples (4 ml) were obtained from all participants following overnight fasting. Two milliliters of blood samples were centrifuged at 2,500 rpm for 3 min to separate the serum from the blood cells. Buffy coats and remaining whole blood samples were stored at −80°C for DNA extraction and other biochemical tests. Triglyceride (TG), total cholesterol (TC), HDL-C, fasting blood sugar (FBS) (Biorex fars, Iran), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) (Pars Azmun, Karaj, Iran) were measured by commercial kits. LDL-C concentration is also calculated using the Friedewald formula (24 (link)): LDL = TC − HDL − 1/5 (TG). Then, biochemical markers were categorized into normal TG (<150 mg/dL) or high TG (≥150 mg/dL), normal LDL-C (<130 mg/dL) or high LDL-C (≥130 mg/dL), normal HDL-C (≥40 mg/dL for men and ≥50 mg/dL for women) or low HDL (<40 mg/dL for men and <50 mg/dL for women) (22 (link)), normal TC (<200 mg/dL) or high TC (≥200 mg/dL) (25 (link)), normal ALT (<47 IU/L for men and <30 IU/L for women) or elevated ALT (>47 IU/L for men and >30 IU/L for women), normal AST (<30 IU/L) or elevated AST (>30 IU/L) (26 ), and normal FBS (<110 mg/dL) or high FBS (≥110 mg/dL) (22 (link)).