EGR-G01 ES cells were generated in the Ikawa Lab [28 (link)] and cultured in KnockOut DMEM (108297–018, Thermo Fisher Scientific) supplemented with 1% Penicillin-Streptomycin- Glutamine, 55 μM 2-mercaptoethanol, 1% Non-Essential Amino Acid Solution (11140–050, Thermo Fisher Scientific), 1% Sodium Pyruvate (11360–070, Thermo Fisher Scientific), 30 μM Adenosine (A4036, Sigma- Aldrich, St. Louis, MO, USA), 30 μM Guanosine (G6264, Sigma-Aldrich), 30 μM Cytidine (C4654, Sigma-Aldrich), 30 μM Uridine (U3003, Sigma-Aldrich), 10 μM Thymidine (T1895, Sigma-Aldrich), 100 U/ml mouse LIF, and 20% FCS (51650–500, Biowest, Nuaillé, France).
Cytidine c4654
Manufactured by Merck Group
Sourced in United States
Cytidine (C4654) is a nucleoside that consists of cytosine attached to a ribose sugar molecule. It is a naturally occurring compound that plays a vital role in various biological processes, including the synthesis of RNA and DNA. As a laboratory reagent, Cytidine (C4654) can be used in a variety of applications, such as biochemical research and molecular biology experiments.
Automatically generated - may contain errors
Lab products found in correlation
Uridine, by Merck Group (3 mentions)
Knockout dmem, by Thermo Fisher Scientific (3 mentions)
Nonessential amino acid solution, by Thermo Fisher Scientific (3 mentions)
Guanosine, by Merck Group (2 mentions)
Adenosine, by Merck Group (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Thymidine, by Merck Group (2 mentions)
Fetal calf serum (fcs), by Biowest (2 mentions)
Adenosine a4036, by Merck Group (1 mentions)
3 protocols using cytidine c4654
1
Generating EGR-G01 ES Cells
2
Generating and Culturing Mouse ES Cells
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EGR-G0146 (link) ES cells were generated in Dr. Ikawa Lab and cultured in KnockOut DMEM (108297-018, Thermo Fisher Scientific) supplemented with 1% Penicillin-Streptomycin-Glutamine, 55 μM 2-mercaptoethanol, 1% Non-Essential Amino Acid Solution (11140-050, Thermo Fisher Scientific), 1% Sodium Pyruvate (11360-070, Thermo Fisher Scientific), 30 μM Adenosine (A4036, Sigma–Aldrich, St. Louis, MO, USA), 30 μM Guanosine (G6264, Sigma–Aldrich), 30 μM Cytidine (C4654, Sigma–Aldrich), 30 μM Uridine (U3003, Sigma–Aldrich), 10 μM Thymidine (T1895, Sigma–Aldrich), 100 U/ml mouse LIF, and 20% fetal bovine serum (FBS; 51650-500, Biowest, Nuaillé, France). HEK293T cell line was gifted from the Dr. Verma Lab and cultured in Dulbecco’s Modified Eagle Medium (DMEM, High Glucose, Pyruvate; Thermo Fisher Scientific, Waltham, MA, USA) containing 10% FBS.
3
Culture Conditions for EGR-G01 ES Cells
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EGR-G01 ES cells were generated in the Ikawa Lab (28) and cultured in KnockOut DMEM (108297-018, Thermo Fisher Scientific) supplemented with 1% Penicillin-Streptomycin-Glutamine, 55 μM 2-mercaptoethanol, 1% Non-Essential Amino Acid Solution (11140-050, Thermo Fisher Scientific), 1% Sodium Pyruvate (11360-070, Thermo Fisher Scientific), 30 μM Adenosine (A4036, Sigma-Aldrich, St. Louis, MO, USA), 30 μM Guanosine (G6264, Sigma-Aldrich), 30 μM Cytidine (C4654, Sigma-Aldrich), 30 μM Uridine (U3003, Sigma-Aldrich), 10 μM Thymidine (T1895, Sigma-Aldrich), 100 U/ml mouse LIF, and 20% FCS (51650-500, Biowest, Nuaillé, France).
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