Fxnki ko c57 bl6

Irp2^−/− and WT littermate control mice were from a mixed genetic background consisting of 129S4/SvJae and C57Bl/6 and were from Tracey Rouault (National Institute of Child Health and Human Development). Sco2^ki/ki (C57BL/6), Sco2^ki/ko (C57BL/6) and WT (C57BL/6) littermate mice were from Eric Schon (Columbia University). Sco2^ki/ki have a Sco2 knock-in (KI) mutation on both alleles and Sco2^ki/ko have a Sco2 knock-in (KI) mutation on one allele and have Sco2 deleted on the other allele (KO)^{37 (link)}. Wild-type (C57BL/6) and Fxn^ki/ko (C57/BL6) were purchased from Jackson Laboratories. Fxn^ki/ko mice harbor one allele of the frataxin (GAA) 230Δneo expansion mutation (Fxntm1.1Pand) on one chromosome, and one allele of the frataxin exon 4-deleted mutation (Fxntm1Mkn) on the homologous chromosome. All animals were housed in the same room and kept under a 12-hour light/dark cycle. The iron content of the standard diet was 200 mg/kg. Power was not explicitly calculated for each experiment. Numbers of mice were typically chosen based on prior experiments. Cages were chosen at random for CS or RA exposures. We did not use a blinded approach during the CS exposure as mice needed to be monitored during and after smoke exposures. In addition, mouse cages exposed to CS displayed a distinctive smell and color and were obvious to the investigators.