Sequencing grade tpck modified trypsin

Sequencing-grade TPCK-modified trypsin was purchased from Promega (Madison, WI, USA). Bradford protein assay kit, ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA) were purchased from Bio-Rad (Hercules, CA, USA). All the other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Influenza virus (A/Puerto Rico/8/1934 H1N1) was propagated in a biosafety level 2 (BL-2) containment facilities. Ultra-pure water was prepared by a MilliQ water purification system (Millpore, Bedford, MA, USA).

Sequencing-grade TPCK-modified trypsin was obtained from Promega (Madison, WI, USA). ACN, formic acid, TCEP, Supel-Tips Ti Pipette Tips were purchased from Sigma-Aldrich (St. Louis, MO, USA). EDTA-free protease inhibitor cocktail tablets were from Roche (Basel, Switzerland). Phosphatase inhibitor cocktail and Ni-NTA resin were from Pierce Biotechnology (Rockford, IL, USA). Anti-HIP-55 antibody was purchased from BD Bioscience (San Diego, CA, USA). All other chemicals, as well as the Bradford protein assay kit, were from Bio-Rad (Hercules, CA, USA); the HIP-55 plasmid was kindly provided by Prof. Haian Fu from Emory University. Ultra-pure water was prepared by a MilliQ water purification system (Millpore, Bedford, MA, USA).