Hplc grade methanol meoh

Phosphate-buffered saline (PBS), methylene blue (MB), and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Palmitic acid (PA) and stearic acid (SA) were supplied by SAMCHUN (Pyeongtaek, Korea). Glycerol monostearate (GMS) was obtained from Kanto Chemical Co, Japan. Inc. (Tokyo, Japan), and Poloxamers (PX) 188 and PX 407 were procured from BASF (Ludwigshafen, Germany). Tween 80 (TW 80) was purchased from Dae Jung Co., Ltd. (Busan, Korea) and chlorophyll-a paste from Shandong Lanmo Biotech Co. Ltd. (Shanghai, China). Methylene chloride (CH₂Cl₂, MC) and DPBF were supplied by Duksan Co. Ltd. (Gyeonggi-do, Korea) and TCI Chemicals (Tokyo, Japan), respectively. Dulbecco’s modified Eagle’s medium (DMEM) was provided by WelGENE (Gyeongsan, South Korea), and penicillin–streptomycin solution (100 ×) and fetal bovine serum (FBS) were purchased from BioWest (Nuaillé, France). The cancer cell lines (HeLa and A549) were obtained from the Korea Cell Line Bank (Seoul, Korea), and the Quanti-MAX WST-8 assay kit from Biomax (Seoul, Republic of Korea). High-performance liquid chromatography (HPLC)-grade methanol (MeOH) was purchased from Honeywell (Seelze, Germany). All other chemicals used were of HPLC grade.

Phosphate-buffered saline (PBS), methylene blue (MB), and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lauric acid (LA) and palmitic acid (PA) were purchased from SAMCHUN (Pyeongtaek, Korea). Glycerol monostearate (GMS) was purchased from Kanto Chemical Co, Japan. Inc. (Tokyo, Japan). Tween 80 (TW 80) was purchased from Dae Jung Co., Ltd. (Busan, Korea). Chlorophyll-a paste was purchased from Shandong Lanmo Biotech Co. Ltd. (Shanghai, China). Methylene chloride (CH₂Cl₂, MC) was purchased from Duksan Co. Ltd. (Ansan-si, Gyeonggi-do, Korea). DPBF was purchased from TCI Chemicals (Tokyo, Japan). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from WelGENE (Gyeongsan, Korea). Penicillin-streptomycin solution (100×) and fetal bovine serum (FBS) were purchased from BioWest (Nuaillé, France). The cancer cell lines (HeLa and A549) were obtained from the Korean Cell Line Bank (Seoul, Korea). The Quanti-MAX WST-8 assay kit was purchased from Biomax (Seoul, Korea). High-performance liquid chromatography (HPLC)-grade methanol (MeOH) was purchased from Honeywell (Seelze, Germany). All other chemicals used were of HPLC grade.

PXCM and PHY were purchased from TCI Chemicals (Tokyo, Japan) and Spectrum Chemical (New Brunswick, NJ, USA), respectively. RTV was provided by Abbott Laboratories (now AbbVie Inc., North Chicago, IL, USA). PVP-VA64 (T_g = 108 °C) was purchased from BASF (Ludwigshafen, Germany). All model compounds are poorly soluble drugs as per the biopharmaceutics classification system (BCS) [50 (link)], as detailed in Table 2. HPLC-grade methanol (MeOH) and acetonitrile (ACN) were purchased from Honeywell (Charlotte, NC, USA). Tri-fluoro acetic acid (TFA) and KH₂PO₄ were purchased from Fisher (Hampton, NH, USA). All materials were used as received. All water (H₂O) used was MilliQ grade with 18.2 MΩ resistance.
Physical mixtures were prepared by weighing an appropriate amount of each component into a scintillation vial and mixed with acoustic mixing at 50 G for 2 min using a LabRAM II (Resodyn Corporation, Butte, MT, USA).