Msd trap vl ion trap mass spectrometer

An Agilent 1100 HPLC system coupled with an MSD Trap VL ion-trap mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) was used for the analysis of anthocyanins [19 (link),38 (link)]. A Kromasil-C18 column (250 × 4.6 mm, 5 µm) was used for the separation of anthocyanins. The mobile phase consisted of (A) 6% (v/v) acetonitrile containing 2% (v/v) formic acid, and (B) 54% (v/v) acetonitrile containing 2% (v/v) formic acid. The column was set at 50 °C and the flow rate was 1.0 mL/min. A sample volume of 30 µL was injected to the system. The gradient was as follows: 0–1 min, 10%B; 1–18 min, 10%B to 25%B; 18–20 min, 25%B isocratic; 20–30 min, 25%B to 40%B; 30–35 min, 40%B to 75%B; and 35–40 min, 70%B to 100%B. The wavelength for the detection was set at 525 nm. Positive electrospray ionization was used with the nebulizer pressure of 35 psi, the temperature of +325 °C, and the dry gas flow rate of 10 mL/min. A full scan mode from m/z 100 to 1500 was recorded. Anthocyanins were tentatively identified by comparing their mass spectrum with the references [39 (link),40 (link)]. The external standard malvidin-3-O-glucoside was used for the quantitation of anthocyanins.

An Agilent 1200 HPLC system coupled with an MSD Trap VL ion-trap mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) was used for the analysis of phenolic compounds [19 (link)]. An Agilent Zorbax SB-C18 reverse column (3 × 50 mm, 1.8 µL) was used for the separation of phenolic compounds. The mobile phase consisted of (A) 1% acetic acid in acetonitrile and (B) 1% acetic acid in water. The column was set at +25 °C with a 1.0 mL/min flow rate. The gradient was as follows: 0–5 min, 0%B to 5%B; 5–10 min, 5%B to 8%B; 10–15 min, 8%B to 12%B; 15–20 min, 12%B to 18%B; 20–22 min, 18%B to 22%B; 22–24 min, 22%B to 35%B; and 24–28 min, 35%B to 100%B. The wavelength on the diode array detector was set at 280 nm. The negative mode was used in the electrospray ionization, and the nebulizer pressure was set at 35 psi. The temperature and flow rate of dry gas were +325 °C and 10 mL/min, respectively. Mass spectrum was recorded using a full scan mode from m/z 100 to 1500. Phenolic compounds were tentatively identified by comparing their mass spectrum with the references [40 (link)]. Catechin, quercetin, gallic acid, caffeic acid, and chlorogenic acid were used for the quantitation of flavanols, flavonols, hydroxybenzoic acids, hydroxycinnamic acids, and chlorogenic acid, respectively.