Chrysin (purity > 98% Figure 1A ) was purchased from Aladdin reagent co., LTD (Shanghai, China). Recombinant mice M-CSF was obtained from R&D Systems (Minneapolis, MN, United States). The purification process of Recombinant RANKL followed the protocol as previously reported (Xu et al., 2000 (link)). The CCK8 assay kits were purchased from Promega (Madison, WI, United States). Antibodies for ERK, phospho-ERK, IκBα, phospho-IκBα, and β-Actin were obtained from Abcam (Cambridge, United Kingdom). Anti-phospho-JNK, anti-JNK, anti-phospho-p38, anti-p38, anti-phospho-NF-κB p65, and anti-NF-κB p65 were obtained from Cell Signaling Technology (Boston, United States).
Cck8 assay kits
Manufactured by Promega
Sourced in United States
The CCK8 assay kits are a colorimetric method for determining cell viability and proliferation. The assay uses a tetrazolium salt that is reduced by living cells to produce a colored formazan dye, which can be quantified using a spectrophotometer. This provides a simple and reliable way to measure cell proliferation, cytotoxicity, and cell viability in a wide range of cell types.
Automatically generated - may contain errors
Lab products found in correlation
Phospho iκbα, by Abcam (1 mentions)
Anti phospho nf κb p65, by Cell Signaling Technology (1 mentions)
Anti nf κb p65, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
β actin, by Abcam (1 mentions)
Phosphate buffer solution (pbs), by Thermo Fisher Scientific (1 mentions)
Qbc939, by American Type Culture Collection (1 mentions)
Goat anti rabbit igg secondary antibody, by Thermo Fisher Scientific (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Qrt pcr and reverse transcription kits, by Transgene (1 mentions)
Ecl developing solution, by Thermo Fisher Scientific (1 mentions)
Huh28, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
2 protocols using cck8 assay kits
1
Osteoclastogenesis Regulation by Chrysin
2
HIBE and Cholangiocarcinoma Cell Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A human normal biliary epithelial cell line (HIBE) and human cholangiocarcinoma cell lines (RBE, QBC939, HuH28) were purchased from ATCC. qRT-PCR and reverse transcription kits were purchased from TransGen Biotech, Beijing, China. CCK-8 assay kits were purchased from Promega, USA. Transwell kits were purchased from Shanghai Yantuo Biotechnology Co., Ltd. Phosphate buffer solution (PBS) and fetal bovine serum (FBS) were purchased from Gibco, Rockville, MD, USA. Trizol reagents were purchased from Beijing Biolab Science and Technology Co., Ltd. Dual-luciferase reporter gene assay (DLRGA) kits were purchased from Beijing Biolab Science and Technology Co., Ltd. Bax, Bcl-2, Caspase-3, and β-Actin antibodies were purchased from Cell Signaling Technology, Boston, Massachusetts, USA. Goat anti-rabbit IgG secondary antibody was purchased from Thermo Fisher Scientific, Shanghai. RIPA and BCA protein assay kits were purchased from Thermo Fisher Scientific, Waltham, MA, USA. An ECL developing solution was purchased from Thermo Fisher Scientific, Waltham, MA, USA. All primers were designed and synthesized by Sangon Biotech (Shanghai) Co., Ltd.
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