Reveraid first strand cdna synthesis

The FOXP2 and AGGF1 mRNA expression were detected by quantitative polymerase chain reaction. Total RNA was extracted by Trizol Reagent (Cwbio, Nanjing, China). RNA (1 μg) was used to generate cDNA with ReverAid First Strand cDNA Synthesis (Thermo Fisher Scientific Inc., Massachusett, USA). After the real-time quantitative PCR was performed with cDNAs and gene-specific primer pairs with Hieff UNICON Power qPCR SYBR Green Master Mix (Yeasen, Beijing, China) by a fluorescence quantitative LightCycler 480 Real-Time PCR system (Roche, Basel, Swenden). 2^−ΔΔCT was used to show the fold change. The primers for FOXP2: 5′- AAG CAT GCT GGC TCA GTC TT -3′ (Forward), 5′- CAC AGG CAC TGC AAA TGT GTT -3′ (Reverse). The primers for AGGF1: 5′- AGC TGG AAA ACG TAG GGA GC -3′ (Forward), 5′- GAA GCT GGA TCG GCG TTT TC -3′ (Reverse). The primers for β-actin: 5′- CAC CAT TGG CAA TGA GCG GTT CC -3′ (Forward), 5′- GTA GTT TCG TGG ATG CCA CAG G -3′ (Reverse).