Alpha-naphthylisothiocyanate (ANIT) and curcumin (CUR) were obtained from Sigma-Aldrich (Sigma-Aldrich, USA). All the bile acid standards were purchased from Sigma-Aldrich as well. Danning tablet used in this study were produced by Shanghai Huchison Pharmaceuticals Co. Ltd (batch No. Z10910040). Ammonium acetate, formic acid, acetonitrile and methanol (HPLC grade) were purchased from Fisher Scientific (Nepean, Ont, Canada). Ultrapure water was prepared by a Milli-Q50 SP Reagent Water System (Millipore Corporation, MA, USA) for the preparation of samples and buffer solutions.
Alpha naphthylisothiocyanate anit
Manufactured by Merck Group
Sourced in United States
Alpha-naphthylisothiocyanate (ANIT) is a chemical compound commonly used in laboratory settings. It serves as a key functional reagent for various analytical and experimental procedures. The core function of ANIT is to provide a reliable source of the isothiocyanate functional group, which is utilized in a range of chemical reactions and assays. While the specific applications of ANIT may vary, its primary role is to facilitate targeted chemical transformations and analyses within the controlled environment of a laboratory.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Curcumin cur, by Merck Group (1 mentions)
Ammonium acetate, by Thermo Fisher Scientific (1 mentions)
Milli q50 sp reagent water system, by Merck Group (1 mentions)
Hplc grade, by Thermo Fisher Scientific (1 mentions)
Tris hcl buffer, by Biodiagnostic (1 mentions)
Alanine transaminase alt, by Merck Group (1 mentions)
Mouse total ba assay kit, by Crystal Chem (1 mentions)
Hematoxylin and eosin, by Merck Group (1 mentions)
Il 13, by Novus Biologicals (1 mentions)
Masson s trichrome stain, by Merck Group (1 mentions)
Elisa maxtm deluxe set, by BioLegend (1 mentions)
Il 10, by Thermo Fisher Scientific (1 mentions)
Ifn γ, by Thermo Fisher Scientific (1 mentions)
4 protocols using alpha naphthylisothiocyanate anit
1
ANIT and Curcumin Pharmacological Evaluation
2
ANIT-Induced Liver Injury and UDCA Treatment
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Alpha-naphthylisothiocyanate (ANIT) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ursofalk (ursodeoxycholic acid) (UDCA) was obtained from MINAPHARM, under license by, Dr Falk Pharma, Germany). Kits for all biochemical parameters serum, and Tris-HCl buffer (pH 7.4) were purchased from Biodiagnostic Company (El Moror St, Dokki, Egypt) . All these chemicals and reagents were of good quality and freshly prepared in vehicles just before use.
3
Recombinant FGF19 and FGF1 Protein Production
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Full-length wide type recombinant human FGF19 (FGF19WT) and the FGF1 variant (FGF1ΔHBS) carrying triple mutations (Lys127Asp, Lys128Gln, and Lys133Val) were expressed in E.coli (BL21) and purified as described in the previous studies (Goetz et al., 2007 (link); Huang et al., 2017 (link)). Mouse Total BA Assay Kit was purchased from Crystal Chem INC. Alpha-naphthylisothiocyanate (ANIT) was purchased from Sigma Aldrich (St. Louis, MO, USA). Alanine transaminase (ALT) and aspartate transaminase (AST) Assay Kits were purchased from Sigma Aldrich (St. Louis, MO, USA) and performed in accordance with the protocols provided with manufacturers. All other reagents were of analytical grade.
4
ANIT-Induced Cholestatic Liver Injury
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Alpha-naphthylisothiocyanate (ANIT) purchased from Sigma-Aldrich (St. Louis, MO, USA). Ursofalk (ursodeoxycholic acid) (UDCA) obtained from UDCA capsule was got from Minapharm Pharmaceutical, Egypt. Kits for all biochemical parameters and Tris-HCl buffer (pH 7.4) were purchased from Biodiagnostic Company (El Moror St., Dokki, Egypt) . Levels of IFN-γ (eBioscience, USA), IL-10 (eBioscience, USA), and IL-13 (Novus Biologicals, USA) were quantified by enzyme-linked immunosorbent assay (ELISA). TNF-α and IL-17A were measured by indirect ELISA MAX TM Deluxe Sets (BioLegend, Inc., San Diego, CA, USA). Hematoxylin and eosin as well as Masson's Trichrome stain were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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