P1873

Manufactured by Merck Group

Sourced in China

The P1873 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use. The core function of the P1873 is to provide a stable and controlled environment for various experimental and analytical procedures.

Automatically generated - may contain errors

Lab products found in correlation

Ab150361, by Abcam (1 mentions) Normal goat serum (ngs), by Fujifilm (1 mentions) F3648, by Merck Group (1 mentions) Fv10i confocal laser scanning microscope, by Olympus (1 mentions) Alexa fluor 594 labeled phalloidin, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Runx2 antibody, by Cell Signaling Technology (1 mentions) Apotome fluorescence microscope, by Zeiss (1 mentions)

2 protocols using p1873

Immunofluorescence Staining and Cell Morphometry

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Cells were washed three times with PBS, fixed for 15 min at room temperature with 4% paraformaldehyde, washed with PBS, and incubated for 1 h at room temperature with 1% normal goat serum (Wako) and 1% BSA (Sigma-Aldrich) in PBS to block nonspecific binding of antibodies before staining with primary antibodies according to standard procedures. Primary antibodies included rabbit monoclonal antibodies to ITGα5 (1:200 dilution; ab150361, Abcam) as well as rabbit polyclonal antibodies to perilipin A/B (1:500 dilution; P1873, Sigma-Aldrich) and to FN (1:800 dilution; F3648, Sigma-Aldrich). Secondary antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 594 were obtained from Molecular Probes (Eugene, OR). F-actin was stained with Alexa Fluor 488– or Alexa Fluor 594–labeled phalloidin (200 U/ml, Molecular Probes) in PBS. Cells were counterstained with Hoechst 33342 (Sigma-Aldrich) at 5 μg/ml and were observed with an FV10i confocal laser-scanning microscope (Olympus, Tokyo, Japan). Using the images of the actin cytoskeleton, the cell boundary was manually traced and the roundness (4 × area/[π × major axis length²]) of the individual cells was quantified with ImageJ/FIJI software (National Institutes of Health, Bethesda, MD).

Uetaki M., Onishi N., Oki Y., Shimizu T., Sugihara E., Sampetrean O., Watanabe T., Yanagi H., Suda K., Fujii H., Kano K., Saya H, & Nobusue H. (2022). Regulatory roles of fibronectin and integrin α5 in reorganization of the actin cytoskeleton and completion of adipogenesis. Molecular Biology of the Cell, 33(9), ar78.

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Histological Evaluation of Bone and Vascular Calcification

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The femora and abdominal aortas were fixed for 48 h with 4% PFA, decalcified for one week in 18% EDTA (for bone only) and embedded in paraffin after dehydration. 5 μm-thick femoral sections were made and processed for PLIN immunostaining using the antibody from Sigma-Aldrich (P1873; 1:300), immunohistochemical staining for OCN using the antibody from Servicebio (ab93876; 1:200; Wuhan, China), and TRAP staining with a kit from Sigma-Aldrich (387A). 5 μm-thick aortic sections were obtained and stained with Von Kossa reagent (G3282; Solarbio), ARS reagent (G1452; Solarbio), or RUNX2 antibody (12556; 1:800; Cell Signaling Technology, Danvers, USA). Secondary antibodies were purchased from Cell Signaling Technology (7074; 1:200) or Jackson Immuno Research (711-545-152 or 711-585-152; 1:400). Images were obtained under an optical microscope (Olympus CX31, Tokyo, Japan) or a Zeiss ApoTome fluorescence microscope. The number of adipocytes per square millimeter of marrow tissue (N. AdCs/Ar/mm²), the numbers of osteoblasts and osteoclasts per millimeter of bone surface (N. OBs/BS/mm and N. OCs/BS/mm), and the percentages of Von Kossa⁺, ARS⁺, and RUNX2⁺ areas for each aortic section were quantified.

Wang Z.X., Luo Z.W., Li F.X., Cao J., Rao S.S., Liu Y.W., Wang Y.Y., Zhu G.Q., Gong J.S., Zou J.T., Wang Q., Tan Y.J., Zhang Y., Hu Y., Li Y.Y., Yin H., Wang X.K., He Z.H., Ren L., Liu Z.Z., Hu X.K., Yuan L.Q., Xu R., Chen C.Y, & Xie H. (2022). Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox. Nature Communications, 13, 1453.

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