Human soluble cd14 scd14 elisa kit

Sera samples of 27 IR, 30 INR, and 17 healthy participants were collected for the measurement of the immune activation markers. These markers were quantified using LEGENDplex™ Human Th Cytokine Panel (Biolegend, San Diego, CA): IL-2, IL-4, IL-6, IL-9, IL10, IL-13, IL-17A, IL-17F, IL-21, IL-22, TNF-α and interferon (IFN)-γ, in line with the manufacturer’s instructions. Human Lipopolysaccharides (LPS) ELISA Kit (CUSABIO; Wuhan, China) and Human soluble CD14 (sCD14) ELISA Kit (MultiSciences, Hangzhou, China) were used to test the plasma LPS and sCD14 following the standard protocols. Two replicates were performed for each assay.

Sera samples of immunological responders and non-responders were collected to measure the bacterial translocation markers. Following the standard protocols, human Lipopolysaccharides (LPS) ELISA Kit (CUSABIO; Wuhan, China) and Human soluble CD14 (sCD14) ELISA Kit (MultiSciences, Hangzhou, China) were used to test plasma LPS and sCD14. Flow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, United States) were used to quantify CD4 + /CD8 + T-cells and HIV-1 RNA, respectively. Fresh anticoagulated whole blood was used to quantify the expressing markers of immune activation (CD25 +, CD38 +, HLADR +, or CD38 + /HLA-DR +) of CD4 + and CD8 + T-cells and immune senescence (CD57 +) by BD FACS Canto II flow cytometer (BD Biosciences, California, United States). The antibodies needed during the experiment were purchased from Biolegend (San Diego, CA), including CD3-FITC, CD4- PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC).